Processing of equine bone marrow using the automated MarrowXpress System: RBC depletion, volume reduction, and mononuclear cell recovery

Veterinary Clinical Pathology

Volumn 40, Issue 4, 2011, Pages 444-449

  Owens, Sean D ^a   Burges, Julie ^b   Johns, Jennifer L ^a   Carrade, Danielle D ^a   Galuppo, Larry D ^a   Librach, Fred ^b   Borjesson, Dori L ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cartilage; Cell separation; Horse; Mesenchymal stem cells; Musculoskeletal; Tendon

Indexed keywords

EQUIDAE;

EID: 83055186523     PISSN: 02756382     EISSN: 1939165X     Source Type: Journal    
DOI: 10.1111/j.1939-165X.2011.00368.x     Document Type: Article

References (26)

1. Crovace A, Lacitignola L, De Siena R, Rossi G, Francioso E. Cell therapy for tendon repair in horses: an experimental study. Vet Res Commun. 2007;31(Suppl 1):281-283.
2. Frisbie DD, Smith RK. Clinical update on the use of mesenchymal stem cells in equine orthopaedics. Equine Vet J. 2010;42:86-89.
3. Richardson LE, Dudhia J, Clegg PD, Smith R. Stem cells in veterinary medicine-attempts at regenerating equine tendon after injury. Trends Biotechnol. 2007;25:409-416.
4. Crovace A, Lacitignola L, Rossi G, Francioso E. Histological and immunohistochemical evaluation of autologous cultured bone marrow mesenchymal stem cells and bone marrow mononuclear cells in collagenase-induced tendonitis of equine superficial digital flexor tendon. Vet Med Int. 2010; 2010: 250978, doi:10.4061/2010/250978.
5. Herthel DJ. Enhanced suspensory ligament healing in 100 horses by stem cells and other bone marrow components. Proceedings of the American Association of Equine Practitioners, San Diego, CA, November 24-28, 2001; 319-321.
6. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143-147.
7. Fortier LA. Stem cells: classifications, controversies, and clinical applications. Vet Surg. 2005;34:415-423.
8. Wilke MM, Nydam DV, Nixon AJ. Enhanced early chondrogenesis in articular defects following arthroscopic mesenchymal stem cell implantation in an equine model. J Orthop Res. 2007;25:913-925.
9. Frisbie DD, Kisiday JD, Kawcak CE, Werpy NM, McIlwraith CW. Evaluation of adipose-derived stromal vascular fraction or bone marrow-derived mesenchymal stem cells for treatment of osteoarthritis. J Orthop Res. 2009;27:1675-1680.
10. Guest DJ, Smith MR, Allen WR. Monitoring the fate of autologous and allogeneic mesenchymal progenitor cells injected into the superficial digital flexor tendon of horses: preliminary study. Equine Vet J. 2008;40:178-181.
11. Nixon AJ, Dahlgren LA, Haupt JL, Yeager AE, Ward DL. Effect of adipose-derived nucleated cell fractions on tendon repair in horses with collagenase-induced tendinitis. Am J Vet Res. 2008;69:928-937.
12. Schnabel LV, Lynch ME, van der Meulen MC, Yeager AE, Kornatowski MA, Nixon AJ. Mesenchymal stem cells and insulin-like growth factor-I gene-enhanced mesenchymal stem cells improve structural aspects of healing in equine flexor digitorum superficialis tendons. J Orthop Res. 2009;27:1392-1398.
13. Smith RK, Korda M, Blunn GW, Goodship AE. Isolation and implantation of autologous equine mesenchymal stem cells from bone marrow into the superficial digital flexor tendon as a potential novel treatment. Equine Vet J. 2003;35:99-102.
14. Vidal MA, Kilroy GE, Johnson JR, Lopez MJ, Moore RM, Gimble JM. Cell growth characteristics and differentiation frequency of adherent equine bone marrow-derived mesenchymal stromal cells: adipogenic and osteogenic capacity. Vet Surg. 2006;35:601-610.
15. Phinney DG, Kopen G, Isaacson RL, Prockop DJ. Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation. J Cell Biochem. 1999;72:570-585.
16. Bruder SP, Kraus KH, Goldberg VM, Kadiyala S. The effect of implants loaded with autologous mesenchymal stem cells on the healing of canine segmental bone defects. J Bone Joint Surg Am. 1998;80:985-996.
17. Quarto R, Mastrogiacomo M, Cancedda R, et al. Repair of large bone defects with the use of autologous bone marrow stromal cells. N Engl J Med. 2001;344:385-386.
18. Lennon DP, Caplan AI. Isolation of rat marrow-derived mesenchymal stem cells. Exp Hematol. 2006;34:1606-1607.
19. Pountos I, Corscadden D, Emery P, Giannoudis PV. Mesenchymal stem cell tissue engineering: techniques for isolation, expansion and application. Injury. 2007;38(Suppl 4):S23-S33.
20. Colleoni S, Bottani E, Tessaro I, et al. Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor. Vet Res Commun. 2009;33:811-821.
21. Lapierre V, Pellegrini N, Bardey I, et al. Cord blood volume reduction using an automated system (Sepax) vs. a semi-automated system (Optipress II) and a manual method (hydroxyethyl starch sedimentation) for routine cord blood banking: a comparative study. Cytotherapy. 2007;9:165-169.
22. Horn P, Bork S, Diehlmann A, et al. Isolation of human mesenchymal stromal cells is more efficient by red blood cell lysis. Cytotherapy. 2008;10:676-685.
23. Russel K, Sellon D, Grindem C. Bone marrow in horses: indications, sample handling and complications. Compend Cont Educ Pract Vet. 1994;16:1359-1365.
24. Arnhold SJ, Goletz I, Klein H, et al. Isolation and characterization of bone marrow-derived equine mesenchymal stem cells. Am J Vet Res. 2007;68:1095-1105.
25. Atland PD, Hathaway WE. Erythrocytes and hemoglobin values: vertebrates. In: Altman PL, Dittmer DS, eds. Biology Data Book. Vol 3. Bethesda, MD: Federation of American Societies for Experimental Biology; 1974:1849-1853.
26. Benjamin ME. Outline of Veterinary Clinical Pathology. 3rd ed. Ames, IA: Iowa State University Press; 1986.