A six-well plate method: Less laborious and effective method for cultivation of obligate anaerobic microorganisms

Microbes and Environments

Volumn 26, Issue 4, 2011, Pages 301-306

  Nakamura, Kohei ^a,b   Tamaki, Hideyuki ^a   Kang, Myung Suk ^a,c   Mochimaru, Hanako ^a   Lee, Sung Taik ^d   Nakamura, Kazunori ^a   Kamagata, Yoichi ^a,e,f  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b GIFU UNIVERSITY   (Japan)

^c National Institute of Biological Resources   (South Korea)

^d Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^e NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^f HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Gellan gum; Methanogen; Sulfate reducing bacterium (SRB); Uncultured anaerobes

Indexed keywords

ANAEROBIC GROWTH; ARCHAEBACTERIUM; ARTICLE; BACTERIAL COUNT; BACTERIUM; COMPARATIVE STUDY; EVALUATION; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION;

ANAEROBIOSIS; ARCHAEA; BACTERIA; COLONY COUNT, MICROBIAL; MICROBIOLOGICAL TECHNIQUES;

EID: 83055161428     PISSN: 13426311     EISSN: 13474405     Source Type: Journal    
DOI: 10.1264/jsme2.ME11120     Document Type: Article

References (25)

1. Altschul, S.F., T.L. Madden, A.A. Schäffer, J. Zhang, Z. Zhang, W. Miller, and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Anderson, I.J., M. Sieprawska-Lupa, A. Lapidus, et al. 2009. Complete genome sequence of Methanoculleus marisnigri. Romesser et al. 1981. type strain JR1. Stand. Genomic Sci. 1:189-196.
3. Beaty, P.S., and M.J. McInerney. 1987. Growth of Syntrophomonas wolfei in pure culture on crotonate. Arch. Microbiol. 147:389-393.
4. Brioukhanov, A.L., and A.I. Netrusov. 2007. Aerotolerance of strictly anaerobic microorganisms and factors of defense against oxidative stress: A review. Appl. Biochem. Microbiol. 43:567-582.
5. Carbonero, F., B.B. Oakley, and K.J. Purdy. 2009. Improving the isolation of anaerobes on solid media: The example of the fastidious Methanosaeta. J. Microbiol. Methods 80:203-205.
6. Chivian, D., E.L. Brodie, E.J. Alm, et al. 2008. Environmental genomics reveals a single-species ecosystem deep within Earth. Science 322:275-278.
7. Doan, N., A. Contreras, J. Flynn, J. Morrison, and J. Slots. 1999. Proficiencies of three anaerobic culture systems for recovering periodontal pathogenic bacteria. J. Clin. Microbiol. 37:171-174.
8. Harris, J.E. 1985. GELRITE as an agar substitute for the cultivation of mesophilic Methanobacterium and Methanobrevibacter species. Appl. Environ. Microbiol. 50:1107-1109.
9. Hungate, R.E., and J. Macy. 1973. The roll-tube method for cultivation of strict anaerobes. Bull. Ecol. Res. Comm. 17:123-125.
10. Kamagata, Y., and E. Mikami. 1991. Isolation and characterization of a novel thermophilic Methanosaeta strain. Int. J. Syst. Bacteriol. 41:191-196.
11. Kamagata, Y., and H. Tamaki. 2005. Cultivation of uncultured fastidious microbes. Microbes Environ. 20:85-91.
12. Mizukami, S., K. Takeda, S. Akada, and T. Fujita. 2006. Isolation and characteristics of Methanosaeta in paddy field soils. Biosci. Biotechnol. Biochem. 70:828-835.
13. Mochimaru, H., H. Yoshioka, H. Tamaki, et al. 2007. Microbial diversity and methanogenic potential in a high temperature natural gas field in Japan. Extremophiles 11:453-461.
14. Patel, G.B. 1984. Characterization and nutritional properties of Methanothrix concilii sp. nov., a mesophilic, aceticlastic methanogen. Can. J. Microbiol. 30:1383-1396.
15. Okabe, S., M. Oshiki, Y. Kamagata, et al. 2010. A Great Leap forward in Microbial Ecology. Microbes Environ. 25:230-240.
16. Plugge, C.M. 2005. Anoxic media design, preparation, and consideration. Methods Enzymol. 397:3-16.
17. Sekiguchi, Y., Y. Kamagata, K. Nakamura, A. Ohashi, and H. Harada. 2000. Syntrophothermus lipocalidus gen. nov., sp. nov., a novel thermophilic, syntrophic, fatty-acid-oxidizing anaerobe which utilizes isobutyrate. Int. J. Syst. Evol. Microbiol. 50:771-779.
18. Sekiguchi, Y. 2006. Yet-to-be cultured microorganisms relevant to methane fermentation processes. Microbes Environ. 21:1-15.
19. Summanen, P.H., M. McTeague, M.-L. Väisänen, C.A. Strong, and S.M. Finegold. 1999. Comparison of recovery of anaerobic bacteria using the Anoxomat®, anaerobic chamber, and GasPak® jar systems. Anaerobe 5:5-9.
20. Tamaki, H., Y. Sekiguchi, S. Hanada, K. Nakamura, N. Nomura, M. Matsumura, and Y. Kamagata. 2005. Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques. Appl. Environ. Microbiol. 71:2162-2169.
21. Tamaki, H., S. Hanada, Y. Sekiguchi, Y. Tanaka, and Y. Kamagata. 2009. Effect of gelling agent on colony formation in solid cultivation of microbial community in lake sediment. Environ. Microbiol. 11:1827-1834.
22. Turova, T.P., B.B. Kuznetsov, E.V. Novikova, A.B. Poltaraus, and T.N. Nazina. 2001. Heterogeneity of nucleotide sequences of 16S ribosomal RNA genes from the Desulfotomaculum kuznetsovii type strain. Mikrobiologiia 70:788-795 (In Russian).
23. Whitman, W.B., D.C. Coleman, and W.J. Wiebe. 1998. Prokaryotes: The unseen majority. Proc. Natl. Acad. Sci. USA 95:6578-6583.
24. Widdel, F., and N. Pfennig. 1977. A new anaerobic, sporing, acetate-oxidizing, sulfate-reducing bacterium, Desulfotomaculum (emend.) acetoxidans. Arch. Microbiol. 112:119-122.
25. Zengler, K. 2009. Central role of the cell in microbial ecology. Microbiol. Mol. Biol. Rev. 73:712-729.