flaB gene as a molecular marker for distinct identification of Borrelia species in environmental samples by the PCR-restriction fragment length polymorphism method

Applied and Environmental Microbiology

Volumn 77, Issue 19, 2011, Pages 7088-7092

  Wodecka, Beata ^a  

^a UNIVERSITY OF SZCZECIN   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

BORRELIA; BORRELIA SPECIES; DENDROGRAMS; ENVIRONMENTAL SAMPLE; FRAGMENT LENGTHS; GENETIC VARIANTS; MOLECULAR MARKER; NEW PROTOCOL; RESTRICTION ENZYMES; SEQUENCE SIMILARITY;

BIOTECHNOLOGY; MICROBIOLOGY;

GENES;

BACTERIAL DNA; DIAGNOSTIC AGENT; FLAB FLAGELLIN; FLAGELLIN; RESTRICTION ENDONUCLEASE;

GENETIC VARIATION; IDENTIFICATION KEY; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; POLYMORPHISM; SAMPLING; SPATIAL DISTRIBUTION; TICK;

ANIMAL; ARTICLE; BORRELIA; CHEMISTRY; CLASSIFICATION; CLUSTER ANALYSIS; DNA SEQUENCE; EUROPE; GENETIC POLYMORPHISM; GENETICS; ISOLATION AND PURIFICATION; IXODES; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; BORRELIA; CLUSTER ANALYSIS; DNA RESTRICTION ENZYMES; DNA, BACTERIAL; ENVIRONMENTAL MICROBIOLOGY; EUROPE; FLAGELLIN; IXODES; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SEQUENCE ANALYSIS, DNA;

EUROPE;

EID: 82955237765     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.05437-11     Document Type: Article

References (21)

1. Derdáková, M., L. Beati, B. Pet'ko, M. Stanko, and D. Fish. 2003. Genetic variability within Borrelia burgdorferi sensu lato genospecies established by PCR-single-strand conformation polymorphism analysis of the rrfA-rrlB intergenic spacer in Ixodes ricinus ticks from the Czech Republic. Appl. Environ. Microbiol. 69:509-516.
2. Fukunaga, M., K. Okada, M. Nakao, T. Konishi, and Y. Sato. 1996. Phylogenetic analysis of Borrelia species based on flagellin gene sequences and its application for molecular typing of Lyme disease borreliae. Int. J. Syst. Bacteriol. 46:898-905.
3. Fukunaga, M., et al. 1995. Genetic and phenotypic analysis of Borrelia miyamotoi sp. nov., isolated from the ixodid tick Ixodes persulcatus, the vector for Lyme disease in Japan. Int. J. Syst. Bacteriol. 45:804-810.
4. Gern, L., E. Rouvinez, L. N. Toutoungi, and E. Godfroid. 1997. Transmission cycles of Borrelia burgdorferi sensu lato involving Ixodes ricinus and/or I. hexagonus ticks and the European hedgehog, Erinaceus europaeus, in suburban and urban areas in Switzerland. Folia Parasitol. (Praha) 44:309-314.
5. Gylfe, A., et al. 1999. Isolation of Lyme disease Borrelia from puffins (Fratercula arctica) and seabird ticks (Ixodes uriae) on the Faeroe Islands. J. Clin. Microbiol. 37:890-896.
6. Kurtenbach, K., et al. 2002. Host association of Borrelia burgdorferi sensu lato-the key role of host complement. Trends Microbiol. 10:74-79.
7. Michalik, J., B. Wodecka, M. Skoracki, B. Sikora, and J. Stańczak. 2008. Prevalence of avian-associated Borrelia burgdorferi s.l. genospecies in Ixodes ricinus ticks collected from blackbirds (Turdus merula) and song thrushes (T. philomelos). Int. J. Mol. Microbiol. 298(Suppl. 1):129-138.
8. Michel, H., et al. 2004. An ospA-PCR/restriction fragment length polymorphism-based method for sensitive detection and reliable differentiation of all European Borrelia burgdorferi sensu lato species and OspA types. Med. Microbiol. Immunol. 193:219-226.
9. Morán Cadenas, F., et al. 2007. Identification of host bloodmeal source and Borrelia burgdorferi sensu lato in field-collected Ixodes ricinus ticks in Chaumont (Switzerland). J. Med. Entomol. 44:1109-1117.
10. Parola, P., and D. Raoult. 2001. Ticks and tickborne bacterial diseases in humans: an emerging infectious threat. Clin. Infect. Dis. 32:897-928.
11. Picken, R. N. 1992. PCR primers and probes derived from flagellin gene sequences for specific detection of the agents of Lyme disease and North American relapsing fever. J. Clin. Microbiol. 30:99-114.
12. Ranka, R., A. Bormane, K. Salmina, and V. Baumanis. 2004. Identification of three clinically relevant Borrelia burgdorferi sensu lato genospecies by PCR-restriction fragment length polymorphism analysis of 16S-23S ribosomal DNA spacer amplicons. J. Clin. Microbiol. 42:1444-1449.
13. Rich, S. M., P. M. Armstrong, R. D. Smith, and S. R. Telford. 2001. Lone star tick-infecting borreliae are most closely related to the agent of bovine borreliosis. J. Clin. Microbiol. 39:494-497.
14. Scoles, G. A., M. Papero, L. Beati, and D. Fish. 2001. A relapsing fever group spirochete transmitted by Ixodes scapularis ticks. Vector Borne Zoonotic Dis. 1:21-34.
15. Sparagano, O. A., et al. 1999. Molecular detection of pathogen DNA in ticks (Acari: Ixodidae): a review. Exp. Appl. Acarol. 23:929-960.
16. Wodecka, B. 2003. Detection of DNA of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks in north-western Poland. Ann. Agric. Environ. Med. 10:171-178.
17. Wodecka, B. 2008. Reservoir host identification in Borrelia-infected Ixodes ricinus ticks, p. 149-157. In A. Buczek and C. Blaszak (ed.), Arthropods. Influence on host. Akapit, Lublin, Poland.
18. Wodecka, B. 2007. Significance of red deer (Cervus elaphus) in the ecology of Borrelia burgdorferi sensu lato. Wiad. Parazytol. 53:231-237.
19. Wodecka, B., A. Leońska, and B. Skotarczak. 2010. A comparative analysis of molecular markers for the detection and identification of Borrelia spirochetes in Ixodes ricinus. J. Med. Microbiol. 59:309-314.
20. Wodecka, B., A. Rymaszewska, M. Sawczuk, and B. Skotarczak. 2009. Detectability of tick-borne agents DNA in the blood of dogs undergoing treatment for borreliosis. Ann. Agric. Environ. Microbiol. 16:9-14.
21. Wodecka, B., and B. Skotarczak. 2005. First isolation of Borrelia lusitaniae DNA from Ixodes ricinus ticks in Poland. Scand. J. Infect. Dis. 37:27-34.