Molecular detection of pathogen DNA in ticks (Acari: Ixodidae): A review

Experimental and Applied Acarology

Volumn 23, Issue 12, 1999, Pages 929-960

  Sparagano, O A E ^a   Allsopp, M T E P ^b   Mank, R A ^a   Rijpkema, S G T ^c   Figueroa, J V ^d   Jongejan, F ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b ONDERSTEPOORT VETERINARY INSTITUTE   (South Africa)

^c NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL   (United Kingdom)

^d Ctro Nac Invest Disciplinarias P   (Mexico)

Author keywords

Anaplasma; Babesia; Borrelia; Cowdria; Dengue virus; Ehrlichia; PCR; Primers; Probes; Rickettsia; TBE virus; Theileria; Ticks

Indexed keywords

DNA ANALYSIS; DNA DETERMINATION; DNA PROBE; MICROORGANISM DETECTION; POLYMERASE CHAIN REACTION; PRIMER DNA; TICK;

CRITICAL ANALYSIS; DETECTION METHOD; LITERATURE REVIEW; PATHOGEN; POLYMERASE CHAIN REACTION;

ANAPLASMA; ANIMALS; ARACHNID VECTORS; BABESIA; BORRELIA; DNA PRIMERS; DNA, BACTERIAL; DNA, PROTOZOAN; DNA, VIRAL; EHRLICHIA; EHRLICHIA RUMINANTIUM; ENCEPHALITIS VIRUSES, TICK-BORNE; NAIROVIRUS; POLYMERASE CHAIN REACTION; THEILERIA; TICK-BORNE DISEASES; TICKS;

EID: 0033493107     PISSN: 01688162     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006313803979     Document Type: Article

References (167)

1. Aboytes-Torres, R. 1992. Development and practical applications of a PCR-based diagnostic system for the study of chronic bovine anaplasmosis. PhD Dissertation. University of Missouri-Columbia, MO, USA.
2. Alekseev, A.N., Dubinina, H.V., Antykova, L.P., Dzhivanyan, T.I., Rijpkema, S.G.T., Verbeek-De Kruif, N. and Cinco, M. 1998. Tick-borne borrelioses pathogen identification in Ixodes ticks collected in St. Petersburg and Kaliningrad regions of Russia. J. Med. Entomol. 35: 136-142.
3. Allsopp, B.A., Baylis, H.A., Allsopp, M.T.E.P., Cavalier-Smith, T., Bishop, R.P., Carrington, D.M., Sohanpal, B. and Spooner, P. 1993. Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences. Parasitol. 107: 157-165.
4. Allsopp, M.T.E.P., Visser, E.S., du Plessis, J.L., Vogel, S.W. and Allsopp, B.A. 1997. Different organisms associated with heartwater as shown by analysis of 16S ribosomal RNA gene sequences. Vet. Parasitol. 71: 283-300.
5. Allsopp, M.T.E.P., Hattingh, C.M., Vogel, S.W. and Allsopp, B.A. 1998. Comparative evaluation of 16S, map1 and pCS20 probes for the detection of Cowdria and Ehrlichia species in ticks. Ann. New-York Acad. Sc. 849: 78-84.
6. Alvarez, J.A., Buening G.M. and Figueroa J.V. 1996. Detection of Babesia bovis in ticks by the PCR assay. Abstracts of the 77th Annual Meeting, Conf. Res. Workers Anim. Dis. Chicago, IL, November, 1996, p. 69.
7. Anderson, B.E., Regnery, R.L., Carlone, G.M., Tzianabos, T., McCade, J.E., Yuan Fu, Z. and Bellini, W.J. 1987. Sequence analysis of the 17-kilodalton-antigen gene from Rickettsia rickettsii. J. Bacteriol. 169: 2385-2390.
8. Anderson, B.E., Sumner, J.W., Dawson, J.E., Tzianabos, T., Greene, C.R., Olson, J.G., Fishbein, D.B., Olsen-Rasmussen, M., Holloway, B.P., George, E.H. and Azad, A.F. 1992. Detection of the etiologic agent of human ehrlichiosis by PCR. J. Clin. Microbiol. 30: 775-780.
9. Anderson, B.E., Sims, K.G., Olson, J.G., Childs, J.E., Piesman, J.F., Happ, C.M., Maupin, G.O. and Johnson, B.J.B. 1993. Amblyomma americanum: a potential vector of human ehrlichiosis. Am. J. Trop. Med. Hyg. 49: 239-244.
10. Assous, M.V., Postic, D., Paul, G., Névot, P., and Baranton, G. 1993. Western blot analysis of sera from Lyme Borreliosis patients according to the genomic species of the Borrelia strains used as antigens. Eur. J. Clin. Microbiol. Infect. Dis. 24: 261-268.
11. Azad, A.F., Sacci Jr., J.B., Nelson, W.M., Dasch, G.A., Schmidtmann, E.T. and Carl, M. 1992. Genetic characterization and transovarial transmission of a typhus-like Rickettsia found in cat fleas. Proc. Natl. Acad. Sci. USA 89: 43-46.
12. Babalis, T., Tselentis, Y., Roux, V., Psaroulaki, A. and Raoult, D. 1994. Isolation and identification of a rickettsial strain related to R. massiliae in Greek ticks. Am. J. Trop. Med. Hyg. 50: 365-372.
13. Bacellar, F., Regnery, R.L., Núncio, M.S. and Filipe, A.R. 1995. Genotypic evaluation of rickettsial isolates recovered from various species of ticks in Portugal. Epidemiol. Infect. 114: 169-178.
14. Balayeva, N.M., Eremeeva, M.E. and Raoult, D. 1994. Genomic identification of R. slovaca among spotted fever group rickettsia isolates from D. marginatus in Armenia. Acta virol. 38: 321-325.
15. Baranton, G., Postic, D., Saint Girons, I., Boerlin, P., Piffaretti, J.C., Assous, M. and Grimont, P.A. 1992. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int. J. Syst. Bacteriol. 42: 378-383.
16. Barbet, A.F. and Allred, D.R. 1991. The mspβ multigene family of Anaplasma marginale: nucleotide sequence analysis of an expressed copy. Infect. Immun. 59: 971-976.
17. Barlough, J., East, N., Rowe, J.D., van Hoosear, K., deRock, E., Bigornia, L. and Rimstad, E. 1994. Double-nested Polymerase chain reaction for detection of caprine arthritis-encephalitis virus proviral DNA in blood, milk, and tissues of infected goats. J. Virol. Methods 50: 101-113.
18. Barthold, S.W. 1998. Spirochetes, ticks and DNA. Parasitol. Today 14: 444.
19. Beati, L., Finidori, J.-P., Gilot, B. and Raoult, D. 1992. Comparison of serologic typing, sodium dodecyl sulfate polyacrylamide gel electrophoresis protein analysis, and genetic restriction fragment length polymorphism analysis for identification of rickettsiae: characterization of two new rickettsial strains. J. Clin. Microbiol. 30: 1922-1930.
20. Beati, L., Humair, P.-F., Aeschlimann, A. and Raoult, D. 1994. Identification of spotted fever group rickettsiae isolated from Dermacentor marginatus and Ixodes ricinus ticks collected in Switzerland. Am. J. Trop. Med. Hyg. 51: 138-148.
21. Beati, L., Roux, V., Ortuño, A., Castella, J., Segura Porta, F. and Raoult, D. 1996. Phenotypic and genotypic characterization of spotted group Rickettsiae isolated from Catalan Rhipicephalus sanguineus ticks. J. Clin. Microbiol. 34: 2688-2694.
22. Bergström, S., Bundoc, V.G. and Barbour, A.G. 1989. Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi. Mol. Microbiol. 3: 479-486.
23. Billings, A.N., Yu, X.-J., Teel, P. and Walker D.H. 1998. Detection of a spotted fever group rickettsia in Amblyomma cajennense in South Texas. J. Med. Entomol. 35: 474-478.
24. Birnboim, H.C. 1988. Rapid extraction of high molecular weight RNA from cultured cells and granulocytes for Northern analysis. Nucleic Acids Res. 16: 1487-1497.
25. Bishop, R.P., Sohanpal, B., Kariuki, D.P., Young, A.S., Nene, V., Baylis, H., Allsopp, B.A., Spooner, P.R., Dolan, T.T. and Morzaria, S.P. 1992. Detection of a carrier state in Theileria parva-infected cattle by polymerase chain reaction. Parasitol. 104: 215-232.
26. Bishop, R., Sohanpal, B., Allsopp, B.A., Spooner, P.R., Dolan, T.T. and Morzaria, S.P. 1993. Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric and ribosomal DNA probes and anti-schizont monoclonal antibodies. Parasitol. 107: 19-31.
27. Black, W.C. IV and Piesman, J. 1994. Phylogeny of hard- and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences. Proc. Natl. Acad. Sci. USA 91: 10034-10038.
28. Canica, M.M., Nato, F., du Merle, L., Mazie, J.C., Baranton, G. and Postic, D. 1993. Monoclonal antibodies for the identification of Borrelia afzelii sp. nov. associated with late cutaneous manifestations of Lyme borreliosis. Scand. J. Infect. Dis. 25: 441-448.
29. Carl, M., Tibbs, C.W., Dobson, M.E., Paparello, S. and Dasch, G.A. 1990. Diagnosis of acute typhus infection using the polymerase chain reaction. J. Infect. Dis. 161: 791-793.
30. Chang, Y.-F., Novosel, V., Chang, C.-F., Kim, J.B., Shin, S.J. and Lein, D.H. 1998. Detection of human granulocytic ehrlichiosis agent and Borrelia burgdorferi in ticks by polymerase chain reaction. J. Vet. Diagn. Invest. 10: 56-59.
31. Chen, P.P., Conrad, P.A., Ole-MoiYoi, O.K., Brown, W.C. and Dolan, T.T. 1991. DNA probes detect T. parva in the salivary glands of R. appendiculatus ticks. Parasitol. Res. 77: 590-594.
32. Chen, S.-H., Dumler, J.S., Bakken, J.S. and Walker, D.H. 1994. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J. Clin. Microbiol. 32: 589-595.
33. Chen, M., Fan, M.Y., Bi, D.Z., Zhang, J.Z. and Huang, Y.P. 1998. Detection of Rickettsia sibirica in ticks and small mammals collected in three different regions of China. Acta Virol. 42: 61-64.
34. Chungue, E., Roche, C., Lefevre, M.F., Barbazan, P. and Chanteau S. 1993. Ultra-rapid, simple, sensitive and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase polymerase chain reaction. J. Med. Virol. 40: 142-145.
35. Cinco, M., Murgia, R., Bonin, S., Padovan, D., and Stanta, G. 1996. Detection of the three species of Borrelia burgdorferi in Ixodes ricinus with the polymerase chain reaction in northern Italy. Alpe Adria Microbiol. J. 5: 253-256.
36. Cinco, M., Padovan, D., Murgia, R., Poldini, L., Frusteri, L., van de Pol, I., Verbeek-De Kruif, N., Rijpkema, S., Taggi, F. and Maroli, M. 1998. Rate of infection of Ixodes ricinus ticks with Borrelia burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii and group VS116 in an endemic focus of Lyme disease in Italy. Eur. J. Clin. Microbiol. Infect. Dis. 17: 90-94.
37. Crampton, A., McKay, I. and Barker, S.C. 1996. Phylogeny of ticks (Ixodida) inferred from nuclear ribosomal DNA. Int. J. Parasitol. 26: 511-517.
38. Dasch, G.A. and Jackson, L.M. 1998. Genetic analysis of isolates of the Spotted Fever group of Rickettsiae belonging to the R. conorii complex. Ann. New-York Acad. Sc. 849: 11-20.
39. de Kok, J.B., d'Oliveira, C. and Jongejan, F. 1993. Detection of the protozoan parasite Theileria annulata in Hyalomma ticks by the polymerase chain reaction. Exp. Appl. Acarol. 17: 839-846.
40. Demaerschalck, I., Ben Messaoud, A., De Kesel, M., Hoyois, B., Lobet, Y., Hoet, P., Bigaignon, G., Bollen, A. and Godfroid, E. 1995. Simultaneous presence of different Borrelia burgdorferi geno-species in biological fluids of Lyme disease patients. J. Clin. Microbiol. 33: 602-608.
41. d'Oliveira, C., van der Weide, M., Jacquiet, P. and Jongejan, F. 1997. Detection of Theileria annulata by PCR in ticks collected from cattle in Mauritania. Exp. Appl. Acarol. 21: 279-291.
42. Drancourt, M., Kelly, P.J., Regnery, R. and Raoult, D. 1992a. Identification of spotted fever group rickettsiae using polymerase chain reaction and restriction-endonuclease length polymorphism analysis. Acta Virol. 36: 1-6.
43. Drancourt, M., Beati, L., Tarasevich, I. and Raoult, D. 1992b. Astrakhan fever rickettsia is identical to Israel tick typhus rickettsia, a genotype of the Rickettsia conorii complex. J. Infect. Dis. 165: 1167-1168.
44. Dumler, J.S. and Bakken, J.S. 1995. Ehrlichial diseases of humans: emerging tick-borne infections. Clin. Infect. Dis. 20: 1102-1110.
45. Dupont, H.T., Cornet, J.-P. and Raoult, D. 1994. Identification of rickettsiae from ticks collected in the Central African Republic using the PCR. Am. J. Trop. Med. Hyg. 50: 373-380.
46. Eiffert, H., Ohlenbusch, A., Christen, H.J., Thomssen, R., Spielman, A. and Matuschka, F.R. 1995. Nondifferentiation between Lyme disease spirochetes from vector ticks and human cerebrospinal fluid. J. Infect. Dis. 171: 476-479.
47. Eremeeva, M., Yu, X. and Raoult, D. 1994a. Differentiation among spotted fever group rickettsiae species by analysis of restriction fragment length polymorphism of PCR-amplified DNA. J. Clin. Microbiol. 32: 803-810.
48. Eremeeva, M.E., Beati, L., Makarova, V.A., Fetisova, N.F., Tarasevich, I.V., Balayeva, N.M. and Raoult, D. 1994b. Astrakhan fever rickettsiae: antigenic and genotypic analysis of isolates obtained from human and Rhipicephalus pumilio ticks. Am. J. Trop. Med. Hyg. 51: 697-706.
49. Eremeeva, M., Balayeva, N., Roux, V., Ignatovich, V., Kotsinjan, M. and Raoult, D. 1995. Genomic and proteinic characterization of strain S, a rickettsia isolated from Rhipicephalus sanguineus ticks in Armenia. J. Clin. Microbiol. 33: 2738-2744.
50. Fahrimal, Y., Goff, W.L. and Jasmer, D.P. 1992. Detection of Babesia bovis carrier cattle by using polymerase chain reaction amplification of parasite DNA. J. Clin. Microbiol. 30: 2091-2096.
51. Faruki, H. 1994. DNA amplification systems for Borrelia burgdorferi. In: PCR-based Diagnostics in Infectious Disease, G.D. Ehrlich and S.J. Greenberg (eds.), pp. 521-536. Blackwell, Boston.
52. Feir, D., Santanello, C.R., Li, B.W., Xie, C.S., Masters, E., Marconi, R. and Weil, G. 1994. Evidence supporting the presence of B. burgdorferi in Missouri. Am. J. Trop. Med. Hyg. 51: 475-482.
53. Figueroa, J.V., Hernández, R. and Buening, G.M. 1992. Use of polymerase chain reaction assay to detect Babesia bigemina in the tick Boophilus microplus. Abstracts 73rd Annual Meeting, Conf. Res. Workers Anim. Dis., Chicago, IL, November, 1992, p. 25.
54. Figueroa, J.V., Chieves, L.P., Johnson, G.S. and Buening, G.M. 1993. Multiplex polymerase chain reaction based assay for the detection of Babesia bigemina, Babesia bovis and Anaplasma marginale DNA in bovine blood. Vet. Parasitol. 50: 69-81.
55. Fukunaga, M., Hamase, A., Okada, K., Inoue, H., Tsuruta, Y., Miyamoto, K. and Nakao, M. 1996. Characterization of spirochetes isolated from ticks (Ixodes tanuki, Ixodes turdus, and Ixodes columnae) and comparison of the sequences with those of Borrelia burgdorferi sensu lato strains. Appl. Environ. Microbiol. 62: 2338-2344.
56. Gage, K.L., Schrumpf, M.E., Karstens, R.H., Burgdorfer, W. and Schwan, T.G. 1994. DNA typing of rickettsiae in naturally infected ticks using a PCR/RFLP system. Am. J. Trop. Med. Hyg. 50: 247-260.
57. Gaunt, M.W., Jones, L.D., Laurenson, K., Hudson, P.J., Reid, H.W. and Gould, E.A. 1997. Definitive identification of louping ill virus by RT-PCR and sequencing in field populations of Ixodes ricinus on the Lochindorb Estate. Arch. Virol. 142: 1181-1191.
58. Gern, L., Estrada-Peña, A., Frandsen, F., Gray, J.S., Jaenson, T.G., Jongejan, F., Kahl, O., Korenberg, E., Mehl, R. and Nuttall, P.A. 1998. European reservoir hosts of Borrelia burgdorferi sensu lato. Zentralbl. Bakteriol. 287: 196-204.
59. Gosteva, V.V., Klitsunova, N.V., Rehacek, J., Kocianova, E., Popov, V.L. and Tarasevich, I.V. 1991. Mixed Rickettsia-virus infection in Dermacentor reticulatus imago. Acta Virol. 35: 174-186.
60. Gray, J.S., Kahl, O., Janetzki-Mittman, C., Stein, J. and Guy, E. 1994. Acquisition of Borrelia burgdorferi by Ixodes ricinus ticks fed on the European hedgehog, Erinaceus europaeus L. Exp. Appl. Acarol. 18: 485-491.
61. Gubbels, J.M., de Vos, A.P., van der Weide, M., Viseras, J., Schouls, L.M., de Vries, E. and Jongejan F. 1999. Simultaneous detection of bovine Theileria and Babesia species using reverse line blot hybridization. J. Clin. Microbiol. 37: 1782-1789.
62. Guttman, D.S., Wang, P.W., Wang, I.-N., Bosler, E.M., Luft, B.J. and Dykhuizen, D.E. 1996. Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single-strand conformation polymorphism analysis. J. Clin. Microbiol. 34: 652-656.
63. Guy, E.C. and Stanek, G. 1991. Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction. J. Clin. Pathol. 44: 610-611.
64. Hermans, P., Dwinger, R.H., Buening, G.M. and Herrero, M.V. 1994. Seasonal incidence and hemoparasite infection rates of ixodid ticks (Acari: Ixodidae) detached from cattle in Costa Rica. Rev. Biol. Trop. 42: 623-632.
65. Hernandez, R., Chen, A.C., Davey, R.B., Ivie, G.W., Wagner, G.G. and George, J.E. 1998. Comparison of genomic DNA in various strains of Boophilus microplus (Acari: Ixodidae). J. Med. Entomol. 35: 895-900.
66. Higgins, J.A. and Azad, A.F. 1995. Use of polymerase chain reaction to detect bacteria in arthropods: A review. J. Med. Entomol. 32: 213-222.
67. Hu, C.M., Humair, P.-F., Wallich, R. and Gern, L. 1997. Apodemus sp. rodents, reservoirs hosts for Borrelia afzelii in an endemic area in Switzerland. Zentralbl Bakt 285, 558-564.
68. Hubálek, Z. and Halouzka, J. 1998. Prevalence rates of Borrelia burgdorferi sensu lato in host-seeking Ixodes ricinus ticks in Europe. Parasitol. Res. 84: 167-172.
69. Hubbard, M.J., Cann, K.J. and Wright, D.J.M. 1995. Validation and rapid extraction of nucleic acids from alcohol-preserved ticks. Exp. Appl. Acarol. 19: 473-478.
70. Hubbard, M.J., Baker, A.S. and Cann, K.J. 1998. Distribution of Borrelia burgdorferi s.l. spirochaete DNA in British ticks (Argasidae and Ixodidae) since the 19th century, assessed by PCR. Med. Vet. Entomol. 12: 89-97.
71. Johnson, B.J.B., Happ, C.M., Mayer, L.W. and Piesman, J. 1992. Detection of B. burgdorferi in ticks by species-specific amplification of the flagellin gene. Am. J. Trop. Med. Hyg. 47: 730-741.
72. Jongejan, F., van Vliet, A.H.M., van der Weide, M. and d'Oliveira, C. 1998. Detection of two tick-borne pathogens (Theileria annulata and Cowdria ruminantium) by the polymerase chain reaction. Proceedings of the Second International Conference on Tick-borne Pathogens, Kruger National Park, Republic of South Africa, 28 August-1 September 1995, pp. 521-525.
73. Kahl, O., Gern, L., Gray, J.S., Guy, E.C., Jongejan, F., Kirstein, F., Kurtenbach, K., Rijpkema, S.G.T. and Stanek, G. 1998. Detection of Borrelia burgdorferi sensu lato in ticks: immunofluoresence assay versus polymerase chain reaction. Zbl. Bakteriol. 287: 205-210.
74. Kawabata, H., Masuzawa, T. and Yanagihara, Y. 1993. Genomic analysis of Borrelia japonica sp. nov. isolated from Ixodes ovatus in Japan. Microbiol. Immunol. 37: 843-848.
75. Kawazu, S.-I., Kamio, T., Sekizaki, T. and Fujisaki, K. 1995. Theileria sergenti and T. buffeli: polymerase chain reaction-based marker system for differentiating the parasite species from infected cattle blood and infected tick salivary gland. Exp. Parasitol. 81: 430-435.
76. Kirstein, F. and Gray, J.S. 1996. A molecular marker for the identification of the zoonotic reservoirs of Lyme borreliosis by analysis of the blood meal in its European vector Ixodes ricinus. Appl. Environ. Microbiol. 62: 4060-4065.
77. Kirstein, F., Rijpkema, S., Molkenboer, M. and Gray, J.S. 1997. The distribution and prevalence of B. burgdorferi genomic groups in Ixodes ricinus ticks in Ireland. Eur. J. Epidemiol. 13: 67-72.
78. Kirvar, E., Ilhan, T., Katzer, F., Wilkie, G., Hooshmand-Rad, P. and Brown, D. 1998. Detection of Theileria lestoquardi (hirci) in ticks, sheep, and goats using the polymerase chain reaction. Ann. New-York Acad. Sc. 849: 52-61.
79. Kozuch, O., Gurycova, D., Lysy, J. and Labuda, M. 1995. Mixed natural focus of tick-borne encephalitis, tularemia and haemorrhagic fever with renal syndrome in west Slovakia. Acta Virol. 39: 95-98.
80. Kron, M.A., Gupta, A. and MacKenzie, C.D. 1991. Identification of related DNA sequences in Borrelia burgdorferi and two strains of Leptospira interrogans by using polymerase chain reaction. J. Clin. Microbiol. 29: 2338-2340.
81. Kubota, S., Sugimoto, C. and Onuma, M. 1996. Population dynamics of Theileria sergenti in persistently infected cattle and vector ticks analysed by a PCR. Parasitol. 112: 437-442.
82. Kurtenbach, K., Sewell, H.S., Ogden, N.H., Randolph, S.E. and Nuttall, P.A. 1998a. Serum complement sensitivity as a key factor in Lyme disease ecology. Infect. Immun. 66: 1248-1251.
83. Kurtenbach, K., Peacey, M., Rijpkema, S.G.T., Hoodless, A.N., Nuttall, P.A. and Randolph, S.E. 1998b. Differential transmission of the genospecies of Borrelia burgdorferi sensu lato by game birds and small rodents in England. Appl. Environ. Microbiol. 64: 1169-1174.
84. Labuda, M., Danielova, V., Jones, L.D. and Nuttall, P.A. 1993. Amplification of tick-borne encephalitis virus infection during co-feeding of ticks. Med. Vet. Entomol. 7: 339-342.
85. Lange, J.V., El Dessouky, A.G., Manor, E., Merdan, A.I. and Azad, A.F. 1992. Spotted fever rickettsiae in ticks from the northern Sinai Governate, Egypt. Am. J. Trop. Med. Hyg. 46: 546-551.
86. Lebech, A.M., Hindersson, P., Vuust, J. and Hansen, K. 1991. Comparison of in vitro culture and polymerase chain reaction for detection of Borrelia burgdorferi in tissue from experimentally infected animals. J. Clin. Microbiol. 29: 731-737.
87. Le Fleche, A., Postic, D., Girardet, K., Péter O. and Baranton, G. 1997. Characterization of Borrelia lusitaniae sp. nov. by 16S ribosomal DNA sequence analysis. Int. J. Syst. Bacteriol. 47: 921-925.
88. Lesnyak, O., Laikovskaya, E., Kufko, I., Bruinink, H., Baranova, N. and Rijpkema, S. 1998. Clinical features of Lyme borreliosis in the middle Urals and the distribution of Borrelia burgdorferi sensu lato species in local Ixodes persulcatus ticks. Zbl. Bakteriol. 288: 111-119.
89. Liebisch, G., Sohns, B. and Bautsch, W. 1998. Detection and typing of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks attached to human skin by PCR. J. Clin. Microbiol. 36: 3355-3358.
90. Liveris, D., Gazumyan, A. and Schwartz, I. 1995. Molecular typing of Borrelia burgdorferi sensu lato by PCR-restriction fragment length polymorphism analysis. J. Clin. Microbiol. 33: 589-595.
91. Livesley, M.A., Carey, D., Gern, L. and Nuttall, P.A. 1994. Problems of isolating Borrelia burgdorferi from ticks collected in United Kingdom foci of Lyme disease. Med. Vet. Entomol. 8: 172-178.
92. Magnarelli, L.A., Stafford III, K.C., Mather, T.N., Yeh, M.-I., Horn, K.D. and Dumler, J.S. 1995. Hemocytic rickettsia-like organisms in ticks: serologic reactivity with antisera to ehrlichiae and detection of DNA of agent of human granulocytic ehrlichiosis by PCR. J. Clin. Microbiol. 33: 2710-2714.
93. Mahan, S.M., Waghela, S.D., McGuire, T.C., Rurangirwa, F.R., Wassink, L.A. and Barbet, A.F. 1992. A cloned DNA probe for Cowdria ruminantium hybridizes with eight heartwater strains and detects infected sheep. J. Clin. Microbiol. 30: 981-986.
94. Mangold, A.J., Bargues, M.D. and Mas-Coma, S. 1998a. Mitochondrial 16S rDNA sequences and phylogenetic relationships of species of Rhipicephalus and other tick genera among Metastriata (Acari, Ixodidae). Parasitol. Res. 84: 478-484.
95. Mangold, A.J., Bargues, M.D. and Mas-Coma, S. 1998b. 18S rRNA gene sequences and phylogenetic relationships of European hard-ticks species (Acari: Ixodidae). Parasitol. Res. 84: 31-37.
96. Manor, E., Ighbarieh, J., Sarov, B., Kassis, I. and Regnery, R. 1992. Human and tick spotted fever group Rickettsia isolates from Israel: a genotypic analysis. J. Clin. Microbiol. 30: 2653-2656.
97. Marconi, R.T. and Garon, C.F. 1992. Development of PCR primer sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis. J. Clin. Microbiol. 30: 2830-2834 (see also erratum in J. Clin. Microbiol. 31: 1026).
98. Marconi, R.T. and Garon, C.F. 1992. Development of PCR primer sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis. J. Clin. Microbiol. 30: 2830-2834 (see also erratum in J. Clin. Microbiol. 31: 1026).
99. McLain, D.K., Wesson, D.M., Oliver Jr., J.H. and Collins, F.H. 1995. Variation in ribosomal DNA internal transcribed spacers 1 among eastern populations of Ixodes scapularis (Acari: Ixodidae). J. Med. Entomol. 32: 353-360.
100. Mehlhorn, H. and Schein, E. 1998. Redescription of Babesia equi Laveran, 1901 as Theileria equi Mehlhorn, Schein 1998. Parasitol. Res. 84: 467-475.
101. Misonne, M.-C. and Hoet, P. 1998. Species-specific plasmid sequences for the PCR identification of the three species of Borrelia burgdorferi sensu lato involved in Lyme disease. J. Clin. Microbiol. 36: 269-272.
102. Misonne, M.-C., Van Impe, G. and Hoet, P.P. 1998. Genetic heterogeneity of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected in Belgium. J. Clin. Microbiol. 36: 3352-3354.
103. Mitchell, G.B.B. and Smith, I.W. 1993. Lyme disease in Scotland: results of a serological study in sheep. Vet. Rec. 133: 66-67.
104. Munderloh, U.G., Madigan, J.E., Dumler, J.S., Goodman, J.L., Hayes, S.F., Barlough, J.E., Nelson, C.M. and Kurtti, T.J. 1996. Isolation of the equine granulocytic ehrlichiosis agent, Ehrlichia equi, in tick cell culture. J. Clin. Microbiol. 34: 664-670.
105. Murphy, G.L., Ewing, S.A., Whitworth, L.C., Fox, J.C. and Kocan, A.A. 1998. A molecular and serologic survey of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma. Vet. Parasitol. 79: 325-339.
106. Nakao, M., Miyamoto, K. and Fukunaga, M. 1994. Lyme disease spirochetes in Japan: enzootic transmission cycles in birds, rodents, and Ixodes persulcatus ticks. J. Infect. Dis. 170: 878-882.
107. Nakao, M. and Miyamoto, K. 1995. Mixed infection of different Borrelia species among Apodemus speciosus mice in Hokkaido, Japan. J. Clin. Microbiol. 33: 490-492.
108. Niebylski, M.L., Schrumpf, M.E., Burgdorfer, W., Fischer, E.R., Gage, K.L. and Schwan T.G. 1997. Rickettsia peacockii sp. nov., a new species infecting wood ticks, Dermacentor andersoni, in Western Montana. Int. J. Syst. Bact. 47: 446-452.
109. Niebylski, M.L., Peacock, M.G. and Schwan, T.G. 1999. Lethal effect of Rickettsia rickettsii on its tick vector (Dermacentor andersoni). Appl. Environ. Microbiol. 65: 773-778.
110. Norris, D.E., Klompen, J.S.H., Keirans, J.E. and Black W.C. IV. 1996. Population genetics of Ixodes scapularis based on mitochondrial 16S and 12S genes. J. Med. Entomol. 33: 78-89.
111. Ohta, M., Kawazu, S.I., Tsuji, N., Terada, Y., Kamio, T. and Fujisaki, K. 1995. Rapid and sensitive method for detection of newly isolated Babesia parasite (Babesia sp. 1) in the anticipated vector-tick using polymerase chain reaction. J. Protozool. Res. 5: 108-117.
112. Olsén, B., Jaenson, T.G.T., Noppa, L., Bunikis, J. and Bergström, S. 1993. A Lyme borreliosis cycle in seabirds and Ixodes uriae ticks. Nature 362: 340-342.
113. Olsén, B., Jaenson, T.G.T. and Bergström, S. 1995. Prevalence of Borrelia burgdorferi sensu latoinfected ticks on migrating birds. Appl. Environ. Microbiol. 61: 3082-3087.
114. Panaccio, M. and Lew, A. 1991. PCR based diagnosis in the presence of 8% (v/v) blood. Nucleic Acids Res. 19: 1151.
115. Parola, P., Vestris, G., Martinez, D., Brochier, B., Roux, V. and Raoult, D. 1999. Tick-borne rickettsiosis in Guadeloupe, the French West Indies: isolation of Rickettsia africae from Amblyomma variegatum ticks and serosurvey in humans, cattle, and goats. Am. J. Trop. Med. Hyg. 60: 888-893.
116. Persing, D.H., Telford III, S.R., Spielman, A. and Barthold, S.W. 1990a. Detection of Borrelia burgdorferi infection in Ixodes dammini ticks with the PCR. J. Clin. Microbiol. 28: 566-572.
117. Persing, D.H., Telford III, S.R., Rys, P.N., Dodge, D.E., White, T.J., Malawista, S.E. and Spielman, A. 1990b. Detection of Borrelia burgdorferi DNA in museum specimens of Ixodes dammini ticks. Science 249: 1420-1423.
118. Persing, D.H., Mathiesen, D., Marshall, W.F., Telford, S.R., Spielman, A., Thomford, J.W. and Conrad, P.A. 1992. Detection of Babesia microti by polymerase chain reaction. J. Clin. Microbiol. 30: 2097-2103.
119. Peter, T.F., Deem, S.L., Barbet, A.F., Norval, R.A.I., Simbi, B.H., Kelly, P.J. and Mahan, S.M. 1995. Development and evaluation of PCR assay for detection of low levels of Cowdria ruminantium infection in Amblyomma ticks not detected by DNA probe. J. Clin. Microbiol. 33: 166-172.
120. Pichon, B., Godfroid, E., Hoyois, B., Bollen, A., Rodhain, F. and Perez-Eid, C. 1995. Simultaneous infection of Ixodes ricinus nymphs by two Borrelia burgdorferi sensu lato species: possible implications for clinical manifestations. Emerg. Infect. Dis. 1: 89-90.
121. Picken, R.N. 1992. Polymerase chain reaction primers and probes derived from flagellin gene sequences for specific detection of the agents of Lyme disease and North American relapsing fever. J. Clin. Microbiol. 30: 99-114.
122. Picken, R.N., Cheng, Y., Han, D., Nelson, J.A., Reddy, A.G., Hayden, M.K., Picken, M.M., Strle, F., Bouseman, J.K. and Trenholme, G.M. 1995. Genotypic and phenotypic characterization of Borrelia burgdorferi isolated from ticks and small animals in Illinois. J. Clin. Microbiol. 33: 2304-2315.
123. Postic, D., Assous, M.V., Grimont, P.A.D. and Baranton, G. 1994. Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int. J. Syst. Bacteriol. 44: 743-752.
124. Postic, D., Korenberg, E., Gorelova, N., Kovalevski, Y.V., Bellenger, E. and Baranton, G. 1997. Borrelia burgdorferi sensu lato in Russia and neighbouring countries: high incidence of mixed isolates. Res. Microbiol. 148: 691-702.
125. Pusterla, N., Leutenegger, C.M., Huder, J.B., Weber, R., Braun, U. and Lutz, H. 1999. Evidence of the human granulocytic ehrlichiosis agent in Ixodes ricinus ticks in Switzerland. J. Clin. Microbiol. 37: 1332-1334.
126. Radulovic, S., Feng, H.-M., Crocquet-Valdes, P., Morovic, M., Dzelalija, B. and Walker, D.H. 1994. Antigen-capture enzyme immunoassay: a comparison with other methods for the detection of spotted fever group rickettsiae in ticks. Am. J. Trop. Med. Hyg. 50: 359-364.
127. Ramelow, C., Süss, J., Berndt, D., Roggendorf, M. and Schreier, E. 1993. Detection of tick-borne encephalitis virus RNA in ticks (Ixodes ricinus) by the PCR. J. Virol. Methods 45: 115-119.
128. Regnery, R.L., Spruill, C.L. and Plikaytis, B.D. 1991. Genotypic identification of Rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J. Bacteriol. 173: 1576-1589.
129. Richter Jr., P.J., Kimsey, R.B., Madigan, J.E., Barlough, J.E., Dumler, J.S. and Brooks, D.L. 1996. Ixodes pacificus (Acari: Ixodidae) as a vector of Ehrlichia equi (Rickettsiales: Ehrlichieae). J. Med. Entomol. 33: 1-5.
130. Rijpkema, S.G.T., Molkenboer, M.J.C.H., Schouls, L.M., Jongejan, F. and Schellekens, J.F.P. 1995. Simultaneous detection and genotyping of three genomic groups of Borrelia burgdorferi sensu lato in Dutch Ixodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes. J. Clin. Microbiol. 33: 3091-3095.
131. Rijpkema, S., Golubic, D., Molkenboer, M., Verbeek-De Kruif, N. and Schellekens, J. 1996a. Identification of four genomic groups of B. burgdorferi sensu lato in I. ricinus ticks collected in a Lyme borreliosis endemic region of Northern Croatia. Exp. Appl. Acarol. 20: 23-30.
132. Rijpkema, S.G.T., Herbes, R.G., Verbeek-De Kruif, N. and Schellekens, J.F.P. 1996b. Detection of four species of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from roe deer (Capreolus capreolus) in The Netherlands. Epidemiol. Infect. 116: 563-566.
133. Rijpkema, S.G.T., Tazelaar, D.J., Molkenboer, M.J.C.H., Noordhoek, G.T., Plantinga, G., Schouls, L.M., and Schellekens J.F.P. 1997. Detection of Borrelia afzelii, Borrelia burgdorferi sensustricto, Borrelia garinii and group VS116 by PCR in skin biopsies of patients with erythema migrans and acrodermatitis chronica atrophicans. Clin. Microbiol. Infect. 3: 109-116.
134. Rosa, P.A. and Schwan, T.G. 1989. A specific and sensitive assay for the Lyme disease spirochete Borrelia burgdorferi using the polymerase chain reaction. J. Infect. Dis. 160: 1018-1029.
135. Rosa, P.A., Hogan, D. and Schwan, T.G. 1991. Polymerase chain reaction analysis identify two distinct classes of Borrelia burgdorferi. J. Clin. Microbiol. 29: 524-532.
136. Saint Girons, I., Gern, L., Gray, J.S., Guy, E.C., Korenberg, E., Nuttall, P.A., Rijpkema, S., Schönberg, A., Stanek, G. and Postic, D. 1998. Identification of Borrelia burgdorferi sensu lato species in Europe. Zentralbl. Bakteriol. 287: 190-195.
137. Schouls, L.M., van de Pol, I., Rijpkema, S.G.T., and Schot, C.S. 1999. Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato, and Bartonella species in Dutch Ixodes ricinus ticks. J. Clin. Microbiol. 37: 2215-2222.
138. Schriefer, M.E. and Azad, A.F. 1994. Changing ecology of Rocky Mountain spotted fever. In: Ecological dynamics of tick-home zoonoses, D.E. Sonenschine and T.N. Mather (eds.), pp. 314-326. Oxford University Press, New York.
139. Schwan, T.G., Schrumpf, M.E., Karstens, R.H., Clover, J.R., Wong, J., Daugherty, M., Struthers, M. and Rosa, P.A. 1993. Distribution and molecular analysis of Lyme disease spirochetes, B. burgdorferi, isolated from ticks throughout California. J. Clin. Microbiol. 31: 3096-3108.
140. Schwartz, I., Varde, S., Nadelman, R.B., Wormser, G.P. and Fish, D. 1997. Inhibition of efficient polymerase chain reaction amplification of Borrelia burgdorferi DNA in blood-fed ticks. Am. J. Trop. Med. Hyg. 56: 339-342.
141. Shiu, S.Y.W., Ayres, M.D. and Gould, E.A. 1991. Genomic sequence of the structural proteins of louping Ill virus: comparative analysis with tick-borne encephalitis virus. Virology 180: 411-415.
142. Sparagano, O. and Watt, D. 1997. Non-systemic infection in Rhipicephalus appendiculatus ticks. Parasitol. Today 13: 201.
143. Sparagano, O. 1998. Theileria species: Biodiversity? Trop. Anim. Hlth. Prod. 30: 141-142.
144. Steere, A.C., Malawista, S.E., Snydman, D.R., Shope, R.E., Andiman, W.A., Ross, M.R. and Steele, F.M. 1977. Lyme arthritis: an epidemic of oligoarticular arthritis in children and adults in three Connecticut communities. Arthritis Rheum. 20: 7-17.
145. Steere, A.C. 1989. Lyme disease. New Engl. J. Med. 321: 586-596.
146. Stich, R.W., Bantle, J.A., Kocan, K.M., Eriks, I.S. and Palmer, G.H. 1991. Preliminary development of a polymerase chain reaction assay for Anaplasma marginale in ticks. Biotechnology Techn. 5: 269-274.
147. Stich, R.W., Sauer, J.R., Bantle, J.A. and Kocan, K.M. 1993a. Detection of Anaplasma marginale (Rickettsiales: Anaplasmataceae) in secretagogue-induced oral secretions of Dermacentor andersoni (Acari: Ixodidae) with the polymerase chain reaction. J. Med. Entomol. 30: 789-794.
148. Stich, R.W., Bantle, J.A., Kocan, K.M. and Fekete, A. 1993b. Detection of Anaplasma marginale in hemolymph of Dermacentor andersoni with the PCR. J. Med. Entomol. 30: 781-788.
149. Stünzer, D., Hubálek, Z., Halouzka, J., Postic, D., Piere, K. and Marth, E. 1998. Prevalence of Borrelia burgdorferi s.l. in Ixodes ricinus ticks from Styria (Austria) and species identification by PCR-RFLP analysis. Zentralbl. Bakteriol. 288: 471-478.
150. Suss, J., Béziat, P., Ramelow, C. and Kahl, O. 1997. Tick-borne encephalitis virus (TBEV)-specific RT-PCR for characterization of natural foci of TBE and for other applications. Zentralbl. Bakteriol. 286: 125-138.
151. Takada, N., Fujita, H., Yano, Y., Tsuboi, Y. and Mahara, F. 1994. First isolation of a rickettsia closely related to Japanese spotted fever pathogen from a tick in Japan. J. Med. Entomol. 31: 183-185.
152. Tzianabos, T., Anderson, B.E. and McDade, J.E. 1989. Detection of Rickettsia rickettsii DNA in clinical specimens by using PCR technology. J. Clin. Microbiol. 27: 2866-2868.
153. Uchida, T., Yan, Y. and Kitaoka, S. 1995. Detection of R. japonica in H. longicornis ticks by restriction fragment length polymorphism of PCR product. J. Clin. Microbiol. 33: 824-828.
154. Van Dam, A.P., Kuiper, H., Vos, K., Widjojokusumo, A., Dejongh, B.M., Spanjaard, L., Ramselaar, A.C.P., Kramer, M.D. and Dankert, J. 1993. Different genospecies of B. burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis. Clin. Infect. Dis. 17: 708-717.
155. Van Vliet, A.H.M., van der Zeijst, B.A.M., Camus, E., Mahan, S.M., Martinez, D. and Jongejan, F. 1995. Use of a specific immunogenic region on the Cowdria ruminantium MAPI protein in a serological essay. J. Clin. Microbiol. 33: 2405-2410.
156. Waghela, S.D., Rurangirwa, F.R., Mahan, S.M., Yunker, C.E., Crawford, T.B., Barbet, A.F., Burridge, M.J. and McGuire, T.C. 1991. A cloned DNA probe identifies Cowdria ruminantium in Amblyomma variegatum ticks. J. Clin. Microbiol. 29: 2571-2577.
157. Wagner, G., Cruz, D., Holman, P., Waghela, S., Perrone, J., Shompole, S. and Rurangirwa, F. 1992. Non-immunologic methods of diagnosis of babesiosis. Mem. Inst. Oswaldo Cruz 87: 193-199.
158. Wang, G., van Dam, A. P., Le Fleche, A., Postic, D., Péter, O., Baranton, G., de Boer, R. and Spanjaard, L. 1997. Genetic and phenotypic analysis of Borrelia valaisiana sp. nov. (Borrelia genomic groups VS116 and M19). Int. J. Syst. Bacteriol. 47: 926-932.
159. Ward, V.K., Marriott, A.C., Booth, T.F., El-Ghorr, A.A. and Nuttall, P.A. 1990. Detection of an arbovirus in an invertebrate and a vertebrate host using the PCR. J. Virol. Methods 30: 291-300.
160. Watt, D., Sparagano, O., Brown, D. and Walker, A. 1997. Use of the PCR for identification and quantification of Theileria parva protozoa in R. appendiculatus. Parasitol. Res. 83: 359-363.
161. Weller, S.J., Baldridge, G.D., Munderloh, U.G., Noda, H., Simser, J. and Kurtti, T.J. 1998. Phylogenetic placement of Rickettsiae from the ticks Amblyomma americanum and Ixodes scapularis. J. Clin. Microbiol. 36: 1305-1317.
162. Wilson, K.H., Blitchington, R.B. and Greene, R.C. 1990. Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction. J. Clin. Microbiol. 28: 1942-1946.
163. Wittenbrink, M.M., Thiele, D. and Krauss, H. 1994. Comparison of dark-field microscopy, culture, and polymerase chain reaction (PCR) for detection of Borrelia burgdorferi in field-collected Ixodes ricinus ticks. Zentralbl. Bakteriol. 281: 183-191.
164. Wood, D.O., Williamson, L.R., Winkler, H.H. and Krause, D.C. 1987. Nucleotide sequence of the Rickettsia prowazekii citrate synthase gene. J. Bacteriol. 169: 3564-3572.
165. Yu, X., Jin, Y., Fan, M., Xu, G., Liu, Q. and Raoult, D. 1993. Genotypic and antigenic identification of two new strains of spotted fever group rickettsiae isolated from China. J. Clin. Microbiol. 31: 83-88.
166. Zahler, M., Gothe, R. and Rinder, H. 1995. Diagnostic DNA amplification from individual tick eggs, larvae and nymphs. Exp. Appl. Acarol. 19: 731-736.
167. Zhang, J.Z., Fan, M.Y. and Bi, D.Z. 1995. Detection of spotted fever group rickettsiae in ticks and rodents by PCR technique in People's Rrepublic of China. Acta Virol. 39: 263-267.