Cloning of novel cellulases from cellulolytic fungi: Heterologous expression of a family 5 glycoside hydrolase from Trametes versicolor in Pichia pastoris

Enzyme and Microbial Technology

Volumn 49, Issue 6-7, 2011, Pages 485-491

  Salinas, Alejandro ^a   Vega, Marcela ^a   Lienqueo, María Elena ^a   Garcia, Alejandro ^a   Carmona, Rene ^a   Salazar, Oriana ^a  

^a UNIVERSITY OF CHILE   (Chile)

Author keywords

Endoglucanase; Heterologous expression; Lignocellulose hydrolysis; Pichia pastoris; Trametes versicolor; White rot fungi

Indexed keywords

ENDOGLUCANASES; HETEROLOGOUS EXPRESSION; PICHIA PASTORIS; TRAMETES VERSICOLOR; WHITE ROT FUNGI;

CELLULOSE; CLONING; ENCODING (SYMBOLS); ENZYME ACTIVITY; HYDROLASES; LIGNIN; PLANTS (BOTANY); SUGARS;

YEAST;

CARBOHYDRATE BINDING MODULE 1; CARBOHYDRATE BINDING PROTEIN; CELLULASE; COMPLEMENTARY DNA; ENDOGLUCANASE II; GLUCAN SYNTHASE; GLYCOSIDASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME DEGRADATION; ENZYME STRUCTURE; ENZYME SYNTHESIS; EXTRACELLULAR SPACE; FUNGUS; FUSARIUM OXYSPORUM; GANODERMA APPLANATUM; GENE INDUCTION; GLOMERELLA GRAMINICOLA; HYDROLYSIS; HYPOCREA JECORINA; LACCARIA BICOLOR; METHYLOTROPHIC YEAST; MUSHROOM; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PICHIA PASTORIS; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; TRAMETES VERSICOLOR;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELLULASES; CLONING, MOLECULAR; DNA, FUNGAL; FUNGAL PROTEINS; GENE EXPRESSION; GENES, FUNGAL; MOLECULAR SEQUENCE DATA; PHYLOGENY; PICHIA; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TRAMETES;

FUNGI; FUSARIUM OXYSPORUM; GANODERMA APPLANATUM; GLOMERELLA GRAMINICOLA; HYPOCREA JECORINA; LACCARIA BICOLOR; PICHIA PASTORIS; TRAMETES HIRSUTA; TRAMETES VERSICOLOR;

EID: 82955198512     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2011.10.003     Document Type: Article

References (38)

1. Dashtban M., Schraft H., Qin W. Fungal bioconversion of lignocellulosic residues; opportunities & perspectives. Int J Biol Sci 2009, 5:578-595.
2. Bhat M.K., Bhat S. Cellulose degrading enzymes and their potential industrial applications. Biotechnol Adv 1997, 15:583-620.
3. Gilkes N.R., Henrissat B., Kilburn D.G., Miller R.C., Warren R.A. Domains in microbial beta-1,4-glycanases: sequence conservation, function, and enzyme families. Microbiol Rev 1991, 55:303-315.
4. Henrissat B., Davies G.J. Structural and sequence-based classification of glycoside hydrolases. Curr Opin Struct Biol 1997, 7:637-644.
5. Boraston A.B., Bolam D.N., Gilbert H.J., Davies G.J. Carbohydrate-binding modules: fine-tuning polysaccharide recognition. Biochem J 2004, 382:769-781.
6. Baldrian P., Valaskova V. Degradation of cellulose by basidiomycetous fungi. FEMS Microbiol Rev 2008, 32:501-521.
7. Vinzant T.B., Adney W.S., Decker S.R., Baker J.O., Kinter M.T., Sherman N.E., et al. Fingerprinting Trichoderma reesei hydrolases in a commercial cellulase preparation. Appl Biochem Biotechnol 2001, 91-93:99-107.
8. Schmoll M., Kubicek C.P. Regulation of Trichoderma cellulase formation: lessons in molecular biology from an industrial fungus. A review. Acta Microbiol Immunol Hung 2003, 50:125-145.
9. Henrissat B., Driguez H., Viet C., Schülein M. Synergism of cellulases from Trichoderma reesei in the degradation of cellulose. Biotechnology 1985, 3:722-726.
10. Nidetzky B., Steiner W., Hayn M., Claeyssens M. Cellulose hydrolysis by the cellulases from Trichoderma reesei: a new model for synergistic interaction. Biochem J 1994, 298(Pt 3):705-710.
11. Pettersson G., Cowling E.B., Porath J. Studies on celluloytic enzymes. I. Isolation of a low-molecular-weight cellulase from Polyporus versicolor. Biochim Biophys Acta 1963, 67:1-8.
12. Sambrook J., Russell D.W. Molecular cloning. A laboratory manual 2001, Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
13. Lee C.C., Wong D.W., Robertson G.H. Cloning and characterization of two cellulase genes from Lentinula edodes. FEMS Microbiol Lett 2001, 205:355-360.
14. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959, 31:426-428.
15. Pezoa R., Cortinez V., Hyvarinen S., Reunanen M., Hemming J., Lienqueo M.E., et al. Use of ionic liquids in the pretreatment of forest and agricultural residues for the production of bioethanol. Cellul Chem Technol 2010, 44:165-172.
16. Xiao Z., Storms R., Tsang A. Microplate-based filter paper assay to measure total cellulase activity. Biotechnol Bioeng 2004, 88:832-837.
17. Larkin M.A., Blackshields G., Brown N.P., Chenna R., McGettigan P.A., McWilliam H., et al. ClustalW and ClustalX version 2. Bioinformatics 2007, 23:2947-2948.
18. Rose T.M., Henikoff J.G., Henikoff S. CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design. Nucleic Acids Res 2003, 31:3763-3766.
19. Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W., et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997, 25:3389-3402.
20. Marchler-Bauer A., Lu S., Anderson J.B., Chitsaz F., Derbyshire M.K., DeWeese-Scott C., et al. CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Res 2011, 39:D225-D229.
21. Page R.D.M. TREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 1996, 12:357-358.
22. Sheppard P.O., Grant F.J., Oort P.J., Sprecher C.A., Foster D.C., Hagen F.S., et al. The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum. Gene 1994, 150:163-167.
23. Merino S.T., Cherry J. Progress and challenges in enzyme development for biomass utilization. Adv Biochem Eng Biotechnol 2007, 108:95-120.
24. Harris P.V., Welner D., McFarland K.C., Re E., Navarro Poulsen J.-C., Brown K., et al. Stimulation of lignocellulosic biomass hydrolysis by proteins of glycoside hydrolase family 61: structure and function of a large, enigmatic family. Biochemistry 2010, 49:3305-3316.
25. Lahjouji K., Storms R., Xiao Z., Joung K.B., Zheng Y., Powlowski J., et al. Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor. Appl Microbiol Biotechnol 2007, 75:337-346.
26. Idogaki H., Kitamoto Y. Purification and some properties of a carboxymethyl cellulase from Coriolus versicolor. Biosci Biotechnol Biochem 1992, 56:970-971.
27. Nozaki K., Seki T., Matsui K., Mizuno M., Kanda T., Amano Y. Structure and characteristics of an endo-beta-1,4-glucanase, isolated from Trametes hirsuta, with high degradation to crystalline cellulose. Biosci Biotechnol Biochem 2007, 71:2375-2382.
28. Toda H., Takada S., Oda M., Amano Y., Kanda T., Okazaki M., et al. Gene cloning of an endoglucanase from the basidiomycete Irpex lacteus and its cDNA expression in Saccharomyces cerevisiae. Biosci Biotechnol Biochem 2005, 69:1262-1269.
29. Ding S.J., Ge W., Buswell J.A. Endoglucanase I from the edible straw mushroom, Volvariella volvacea. Purification, characterization, cloning and expression. Eur J Biochem 2001, 268:5687-5695.
30. Takashima S., Nakamura A., Masaki H., Uozumi T. Cloning, sequencing, and expression of a thermostable cellulase gene of Humicola grisea. Biosci Biotechnol Biochem 1997, 61:245-250.
31. Saloheimo M., Lehtovaara P., Penttila M., Teeri T.T., Stahlberg J., Johansson G., et al. EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme. Gene 1988, 63:11-22.
32. Linder M., Teeri T. The roles and function of cellulose-binding domains. J Biotechnol 1997, 57:15-28.
33. Linder M., Mattinen M.L., Kontteli M., Lindeberg G., Stahlberg J., Drakenberg T., et al. Identification of functionally important amino acids in the cellulose-binding domain of Trichoderma reesei cellobiohydrolase I. Protein Sci 1995, 4:1056-1064.
34. Clarke A.J., Drummelsmith J., Yaguchi M. Identification of the catalytic nucleophile in the cellulase from Schizophyllum commune and assignment of the enzyme to Family 5, subtype 5 of the glycosidases. FEBS Lett 1997, 414:359-361.
35. De Schutter K., Lin Y.-C., Tiels P., Van Hecke A., Glinka S., Weber-Lehmann J., et al. Genome sequence of the recombinant protein production host Pichia pastoris. Nat Biotechnol 2009, 27:561-566.
36. Macauley-Patrick S., Fazenda M.L., McNeil B., Harvey L.M. Heterologous protein production using the Pichia pastoris expression system. Yeast 2005, 22:249-270.
37. Quay D.H.X., Bakar F.D.A., Rabu A., Said M., Illias R.M., Mahadi N.M., et al. Overexpression, purification and characterization of the Aspergillus niger endoglucanase, EglA, in Pichia pastoris. Afr J Biotechnol 2011, 10:2101-2111.
38. Ding S.-J., Ge W., Buswell J.A. Secretion, purification and characterisation of a recombinant Volvariella volvacea endoglucanase expressed in the yeast Pichia pastoris. Enzyme Microb Technol 2002, 31:621-626.