ProteoChip-based screening system of novel inhibitors against the interaction of VEGF165 and β-Amyloid1-42

Biochip Journal

Volumn 5, Issue 4, 2011, Pages 299-303

  Park, Chan Won ^a,b   Bang, Ji Young ^a,b   Choi, Youngjin ^a,b   Kang, In Cheol ^a,b  

^a Innopharmascreen Inc   (South Korea)

^b Hoseo University   (South Korea)

Author keywords

Alzheimer's disease; ProteoChip ; SH SY5Y cells; Amyloid

Indexed keywords

ENDOTHELIAL CELLS; NEURODEGENERATIVE DISEASES;

ALZHEIMER'S DISEASE; AMYLOID PLAQUES; HEPARIN BINDING DOMAINS; INTERACTION SYSTEMS; NEURONAL CELL; NEUROTOXICITY; SCREENING METHODS; SCREENING SYSTEM; VASCULAR ENDOTHELIAL GROWTH FACTOR;

GLYCOPROTEINS;

AMYLOID BETA PROTEIN[1-42]; IPS 04001; IPS 04002; IPS 04003; IPS 04004; NEUROPROTECTIVE AGENT; UNCLASSIFIED DRUG; VASCULOTROPIN 165;

ARTICLE; CELL PROLIFERATION; CELL SURVIVAL; COMPETITIVE INHIBITION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG SCREENING; HEPARIN BINDING DOMAIN; HUMAN; HUMAN CELL; MICROARRAY ANALYSIS; NERVE CELL; NEUROTOXICITY; PROTEIN DOMAIN; PROTEIN IMMOBILIZATION; PROTEIN PROTEIN INTERACTION;

EID: 82755186849     PISSN: 19760280     EISSN: 20927843     Source Type: Journal    
DOI: 10.1007/s13206-011-5402-y     Document Type: Article

References (13)

1. Parihar, M. S. & Hemnani, T. Alzheimer's disease pathogenesis and therapeutic interventions. J. Clin. Neurosci. 11, 456-467 (2004).
2. Suh, Y. H. & Checler, F. Amyloid Precursor Protein, Presenilins, and α-Synuclein: Molecular Pathogenesis and Pharmacologycal Applications in Alzheimer's Disease. Pharmacol. Rev. 54, 469-525 (2002).
3. Yankner, B. A. Mechanisms of Neuronal Degeneration in Alzheimer's Disease. Neuron16, 921-932 (1996).
4. Lee, S. H. et al. The Change of Expression in Amyloid Precursor Protein mRNA and Amyloid Precursor Protein by Transient Ischemia. J. Korean Neurosurg. Soc. 27, 1481-1489 (1998).
5. Pike, C. J., Burdick, D., Walencewicz, A. J., Glabe, C. G. & Cotman, C. W. Neurodegeneration Induced by β-Amyloid Peptides in vitro: The Role of Peptide Assembly State. J. Neurosci. 13, 1676-1687 (1993).
6. Suh, Y. H. An Etiological Role of Amyloidogenic Carboxyl-Terminal Fragments of the β-Amyloid Precursor Protein in Alzheimer's Disease. J. Neurochem. 68, 1781-1791 (1997).
7. Yang, S. P., Kwon, B. O., Gho, Y. S. & Chae, C. B. Specific interaction of VEGF165 with β-amyloid, and its protective effect on β-amyloid-induced neurotoxicity. J. Neurochem. 93, 118-127 (2005).
8. Robinson, C. J. & Stringer, S. E. The splice variants of vascular endothelial growth factor (VEGF) and their receptors. J. Cell Sci. 114, 853-865 (2001).
9. Yankner, B. A., Duffy, L. K. & Kirschner, D. A. Neurotrophic and neurotoxic effects of amyloid beta protein: reversal by tachykinin neuropeptides. Science250, 279-282 (1990).
10. Lee, Y. S. et al. ProteoChip: A highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein-protein interaction studies. Proteomics3, 2289-2304 (2003).
11. Lee, Y. S., Kang, D. K., Chang, S. I., Han, M. H. & Kang, I. C. High-throughput screening of novel peptide inhibitors of an integrin receptor from the hexapeptide library by using a protein microarray chip. J. Biomol. Screen. 9, 687-694 (2004).
12. Kim, E. Y., Bang, J. Y., Chang, S. I. & Kang, I. C. A novel integrin α5β1 antagonistic peptide, A5-1, screened by Protein Chip system as a potent angiogenesis inhibitor. Biochem. Biophys. Res. Commun. 377, 1288-1293 (2008).
13. Kim, E. Y. & Kang, I. C. Protein Chip-based Dipeptidyl Peptidase IV Assay System. BioChp J. 3, 249-253 (2009).