Optimization of Brassica napus (canola) explant regeneration for genetic transformation

New Biotechnology

Volumn 29, Issue 1, 2011, Pages 144-155

  Maheshwari, Priti ^a   Selvaraj, Gopalan ^b   Kovalchuk, Igor ^a  

^a UNIVERSITY OF LETHBRIDGE   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AGROBACTERIUM; BENZYLADENINE; BINARY VECTORS; BRASSICA NAPUS; COMMERCIAL PRODUCTIONS; DINITROPHENYLHYDRAZINE; DONOR PLANTS; FIREFLY LUCIFERASE; GENETIC TRANSFORMATION; GENETICALLY MODIFIED; HYPOCOTYL EXPLANTS; LUCIFERASE ASSAYS; MICROSPORE CULTURE; OIL SEED CROPS; PLANTLETS; REGENERATION CAPACITY; REGENERATION MEDIUM; REPORTER GENE; SOMATIC EMBRYO; TRANSGENESIS;

CELL CULTURE; CROPS; CULTIVATION; GENES; NUCLEIC ACIDS;

PLANTS (BOTANY);

2,4 DINITROPHENYLHYDRAZINE; 6 N BENZYLADENINE; LUCIFERASE;

ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; GENETIC TRANSFORMATION; NONHUMAN; PLANT REGENERATION; PRIORITY JOURNAL; RAPESEED; RHIZOBIUM RADIOBACTER; SOMATIC EMBRYOGENESIS;

AGRICULTURE; AGROBACTERIUM TUMEFACIENS; BRASSICA NAPUS; CROPS, AGRICULTURAL; GENE TRANSFER TECHNIQUES; GENETIC ENGINEERING; HYPOCOTYL; PLANT SHOOTS; REGENERATION; TRANSFORMATION, GENETIC;

AGROBACTERIUM; BRASSICA NAPUS; BRASSICA NAPUS VAR. NAPUS;

EID: 82755161017     PISSN: 18716784     EISSN: 18764347     Source Type: Journal    
DOI: 10.1016/j.nbt.2011.06.014     Document Type: Article

References (50)

1. Giddings G., et al. Transgenic plants as factories for iopharmaceuticals. Nat. Biotechnol. 2000, 18:1151-1155.
2. Thorpe T.A. Organogenesis in vitro: structural, physiological, and biochemical aspects. Int. Rev. Cytol. 1980, Suppl II A:71-111.
3. De Block M., et al. Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol. 1989, 91:694-701.
4. Ledig F.T., Sederoff R.R. International Proceedings of the 18th South Forest Tree Improvement Conference 1985, 4-13.
5. Fry J., et al. Transformation of Brassica napus with Agrobacterium tumefaciens based vectors. Plant Cell Rep. 1987, 6:321-325.
6. Moloney M.M., et al. High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep. 1989, 8:238-242.
7. Boulter M.E., et al. Transformation of Brassica napus L. (oilseed rape) using Agrobacterium tumefaciens and Agrobacterium rhizogenes a comparison. Plant Sci. 1990, 70:91-99.
8. Thomzik J.E., Hain R. Transgeinc Brassica napus plants obtained by co-cultivation of protoplasts with Agrobacterium tumefaciens. Plant Cell Rep. 1990, 9:233-236.
9. Phogat S.K., et al. High frequency regeneration of Brassica napus varieties and genetic transformation stocks containing fertility restorer genes for two cytoplasmic male sterility systems. J. Plant. Biochem. Biotechnol. 2000, 9:73-79.
10. Guan C.Y., et al. Breeding and agronomic characters of Bt transgenic insect resistant Brassica napus lines. Cruciferea Newsletter 2001, 23:43-44.
11. Moghaieb R.E.A., et al. A reproducible protocol for regeneration and transformation in canola (Brassica napus L.). Afr. J. Biotechnol. 2006, 5:143-148.
12. Bhalla P.L., Singh M.B. Agrobacterium-mediated transformation of Brassica napus and Brassica oleraciea. Nat. Protoc. 2008, 3:181-189.
13. Golz C., et al. transfer of hygromycin resistance into Brassica napus using total DNA of a transgene B. nigra line. Plant Mol. Biol. 1990, 15:475-483.
14. Chen J.L., Beversdorf W.D. A combination of microprojectile bombardment and DNA imbibition enhances transformation frequency of cano1a (Brassica napus L.). Theor. Appl. Genet. 1994, 8:187-192.
15. Chapel M., Glimelius K. Temporary inhibition of cell wall synthesis improves the transient expression of the GUS gene in Brassica napus mesophyll protoplasts. Plant Cell Rep. 1990, 9:105-108.
16. Jones-Villeneuve E., et al. Assessment of microinjection for introducing DNA into uninuclear microspores of rapeseed. Plant Cell Tissue Organ Cult. 1995, 40:97-100.
17. Hu Q., et al. Plant regeneration capacity of mesophyll protoplasts from Brassica napus and related species. Plant Cell Tissue Organ Cult. 1999, 59:189-196.
18. Huang B. Genetic manipulation of microspores derived embryos. In Vitro Cell Dev. Biol. 1992, 28:53-58.
19. Souvre A., et al. Transformation of rape (Brassica napus L.) through the haploid embryogenesis pathway. Acta Soc. Bot. Pol. 1996, 65:194-195.
20. Palmer C.E., Keller W.A. Pollen embryos. Pollen Embryos 1997, 392-422. Cambridge University Press. K.R. Shivanna, V.K. Sawhney (Eds.).
21. Pua E.C., et al. Transgenic plants of Brassica napus L. Biotechnology 1991, 5:815-817.
22. Sheikholeslam S.N., Weeks D.P. Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens. Plant Mol. Biol. 1987, 8:291-298.
23. Cardoza V., Stewart C.N. Increased Agrobacterium mediated transformation and rooting efficiencies in canola (Brassica napus L.) from hypocotyl segment explants. Plant Cell Rep. 2003, 21:599-604.
24. Wang W.C., et al. Development of a novel Agrobacterium mediated transformation method to recover transgenic Brassica napus plants. Plant Cell Rep. 2003, 22:274-281.
25. Khan M.R., et al. High frequency shoot regeneration and Agrobacterium mediated DNA transfer in Canola (Brassica napus L.). Plant Cell Tissue Organ Cult. 2003, 75:223-231.
26. Ovesna J., et al. Factors influencing the regeneration capacity of oilseed rape and cauliflower in transformation experiments. Biol. Plant. 1993, 35:107-112.
27. Ono Y., et al. effect of genotype on shoot regeneration from cotyledonary explants of rapeseed (Brassica napus L.). Plant Cell Rep. 1994, 14:13-17.
28. Takasaki T., et al. Effects of various factors (hormone combinations, genotypes and antibiotics) on shoot regeneration from cotyledon explants in Brassica rapa L. Plant Tissue Cult. Lett. 1996, 13:177-180.
29. Poulsen G.B. Genetic transformation of Brassica. Plant Breed. 1996, 115:209-225.
30. Kamal G.S., et al. Effects of genotype, explant type and nutrient medium components on canola (Brassica napus L.) shoot in vitro organogenesis. Afr. J. Biotechnol. 2007, 6:861-867.
31. Dunwell J.M. In vitro regeneration from excised leaf discs of Brassica species. J. Exp. Bot. 1981, 32:789-799.
32. Polsoni L., et al. Large scale microspore culture technique for mutation-selection studies in Brassica napus. Can. J. Bot. 1988, 66:1681-1685.
33. Sharma K.K., Thorpe T.A. In vitro regeneration of shoot buds and plantlets from seedling root segments of Brassica napus L. Plant Cell Tissue Organ Cult. 1989, 18:129-141.
34. Sharma K.K., et al. Factors affecting high frequency differentiation of shoots and roots from cotyledon explants of Brassica juncea (L.) Czern. Plant Sci. 1990, 66:247-253.
35. Zihang Y., Bhalla P.L. In vitro shoot regeneration from commercial cultivars of Australian canola (Brassica napus L.). Austr. J. Agric. Res. 2004, 55:753-756.
36. Cardoza V., Stewart C.N. Agrobacterium mediated transformation of canola. Transgenic Crops of the World-Essential Protocols 2004, 379-387. Kluwer Academic Publishers. I.S. Curtis (Ed.).
37. Halfhill M.D., et al. Expression of GFP and Bt transgenes in Brassica napus and hybridization with Brassica rapa. Theor. Appl. Genet. 2001, 103:659-667.
38. Murashige T., Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 1962, 15:473-497.
39. Ali H., et al. In vitro regeneration of Brassica napus L., cultivars (star, cyclone, and westar) from hypocotyls and cotyledonary leaves. Pak. J. Bot. 2007, 39:1251-1256.
40. Skoog F., Miller C.O. Chemical regulation of growth and organ formation in plant tissue cultures in vitro. Symp. Soc. Exp. Biol. 1957, 11:118-131.
41. Khan M.R., et al. High frequency shoot regeneration from hypocotyls of Canola (Brassica napus L.) cv. Dunkled. Plant Tissue Cult. 2002, 12:131-138.
42. Larkin P.J., Scowcroft W.R. Somaclonal variation - a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 1981, 60:197-214.
43. 3 and aminoethoxyvinyglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant Brassica genotypes. Plant Cell Rep. 1990, 9:195-198.
44. Radke S.E., et al. Transformation of Brassica napus L. using Agrobacterium tumefaciens: developmentally regulated expression of a reintroduced napin gene. Theor. Appl. Genet. 1988, 75:685-694.
45. 3 and genotype. Plant Cell Rep. 2005, 24:649-654.
46. Zhang F.L., et al. Medium and genotype factors influencing shoot regeneration from cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant Cell Rep. 1998, 17:780-786.
47. Jonoubi P., et al. Efficient regeneration of Brassica napus L. hypocotyls and genetic transformation by Agrobacterium tumefaciens. Biol. Plant. 2005, 49:175-180.
48. Wang Y.P., et al. Production of fertile transgenic Brassica napus by Agrobacterium-meditated transformation of protoplasts. Plant Breed. 2005, 124:1-5.
49. Zebarjadi A., et al. Transformation of rapeseed (Brassica napus L.) plants with sense and antisense constructs of the fatty acid elongase gene. Iran. J. Biotechnol. 2006, 4:79-87.
50. Mashayekhi M., et al. Gene transformation potential of commercial canola (Brassica napus L.) cultivars using cotyledon and hypocotyls explants. Afr. J. Biotechnol. 2008, 7:4459-4463.