Agrobacterium-mediated transformation of Brassica napus and Brassica oleracea

Nature Protocols

Volumn 3, Issue 2, 2008, Pages 181-189

  Bhalla, Prem L ^a   Singh, Mohan B ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CABBAGE; CAULIFLOWER; CULTIVAR; CULTURE MEDIUM; GENETIC ENGINEERING; INOCULATION; NONHUMAN; PLANT GENETICS; PLANT GROWTH; PLANT TISSUE CULTURE; PRIORITY JOURNAL; RAPESEED; RHIZOBIUM; SEEDLING; SHOOT; TRANSGENIC PLANT; VARIETAS;

BRASSICA; GENE TRANSFER TECHNIQUES; PLANTS, GENETICALLY MODIFIED; RHIZOBIUM RADIOBACTER; TRANSFORMATION, GENETIC;

AGROBACTERIUM; BRASSICA; BRASSICA NAPUS; BRASSICA NAPUS VAR. NAPUS; BRASSICA OLERACEA; BRASSICA OLERACEA VAR. BOTRYTIS; BRASSICA OLERACEA VAR. CAPITATA; BRASSICA OLERACEA VAR. ITALICA;

EID: 39449117963     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.527     Document Type: Article

References (35)

1. Østergaard, L. et al. Pod shatter-resistant Brassica fruit produced by ectopic expression of the FRUITFULL gene. Plant Biotech J. 4, 45-51 (2006).
2. Cardoza, V. & Stewart, N.C. Canola (Brassica napus L.). in Methods in Molecular Biology Vol. 343 (ed. Wang, K.) 257-265 (Humana Press Inc., Totowa, New Jersey, (2006)).
3. Poulsen, G.B. Genetic transformation of Brassica Plant Breed. 115, 209-225 (1996).
4. Puddephat, I.J. et al. Transformation of Brassica oleracea L: A critical review. Mol. Breed. 2, 185-210 (1996).
5. Mukhopadhyay, A.R. et al. Efficient regeneration of Brassica oleracea hypocotyl protoplasts and high-frequency genetic transformation by direct DNA uptake. Plant Cell Rep. 10, 375-379 (1991).
6. Radchuk, V.V. et al. Genetic transformation of cauliflower ( Brassica oleracea var botrytis) by direct DNA uptake into mesophyll protoplasts. Physiol. Plant. 114, 429-438 (2002).
7. Eimert, K. & Siegemund, F. Transformation of cauliflower (Brassica oleracea var botrytis): An experimental survey. Plant Mol. Biol. 19, 485-490 (1992).
8. Bergman, P. & Glimelius, K. Electroporation of rapeseed protoplast--transient and stable transformation. Physiol. Plant. 88, 604-611 (1993).
9. Herve, C. et al. Molecular analysis of transgenic rapeseed plants obtained by direct gene transfer of two separate plasmids containing, respectively the cauliflower mosaic virus coat protein gene and a selectable marker gene. Plant Sci. 91, 181-193 (1993).
10. Jones-Villeneuve, E. et al. Assessment of microinjection for introducing DNA into uninuclear microspores of rapeseed. Plant Cell Tissue Organ Cult. 40, 97-100 (1995).
11. Chen, J.L. & Beversdorf, W.D. A combined use of microprojectile bombardment and DNA imbibition enhances transformation frequency of canola (Brassica napus L.). Theor. Appl. Genet. 88, 187-192 (1994).
12. Nehlin, L. et al. Transient β-gus and gfp expression and viability analysis of microprojectile bombarded microspores of Brassica napus L. J. Plant Physiol. 156, 175-183 (2000).
13. Christey, M.C. & Sinclair, B.K. Regeneration of transgenic kale ( Brassica oleracea var acephala), rape (B. napus) and turnip (B. campestris var rapifera) plants via Agrobacterium rhizogenes-mediated transformation. Plant Sci. 87, 161-169 (1992).
14. Metz, T.D., Dixit, R. & Earle, E.D. Agrobacterium tumefaciens mediated transformation of broccoli (Brassica oleracea var italica) and cabbage (B. oleracea var capitata). Plant Cell Rep. 15, 287-292 (1995).
15. Henzi, M.X., Christey, M.C. & McNeil, D.L. Factors that influence Agrobacterium rhizogenes-mediated transformation of broccoli ( Brassica oleracea L. var italica). Plant Cell Rep. 19, 994-999 (2000).
16. Puddephat, I.J. et al. Recovery of phenotypically normal transgenic plants of Brassica oleracea upon Agrobacterium rhizogenes-mediated co-transformation and selection of transformed hairy roots by GUS assay. Mol. Breed. 7, 229-242 (2001).
17. Oldacres, A.M., Newbury, H.J. & Puddephat, I.J. OTLs controlling the production of transgenic and adventitious roots in Brassica oleracea following treatment with Agrobacterium rhizogenes. Theor. Appl. Genet. 111, 479-488 (2005).
18. Sparrow, P.A.C. et al. Genetic analysis of Agrobacterium tumefaciens susceptibility in Brassica oleracea. Theor. Appl. Genet. 108, 644-650 (2004).
19. Sparrow, P.A.C. et al. Genetic analysis of in vitro shoot regeneration from cotyledonary petioles of Brassica oleracea. Theor. Appl. Genet. 108, 1249-1255 (2004).
20. Rommens, C.M. et al. Crop improvement through modification of the plant's own genome. Plant Physiol. 135, 421-431 (2004).
21. Nielsen, K.M. Transgenic organisms - time for conceptual diversification. Nat. Biotechnol. 21, 227-228 (2003).
22. de Block, M., de Brouwer, D. & Tenning, P. Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of bar and neo genes in transgenic plants. Plant Physiol. 91, 694-701 (1989).
23. Moloney, M.M., Walker, J.M. & Sharma, K.K. High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep. 8, 238-242 (1989).
24. Barfield, D.G. & Pua, E.C. Gene transfer in plants of Brassica juncea using Agrobacterium tumefaciens-mediated transformation. Plant Cell Rep. 10, 308-314 (1991).
25. Radke, S.E., Tumer, J.C. & Facciotti, D. Transformation and regeneration of Brassica rapa using Agrobacterium tumefaciens. Plant Cell Rep. 11, 499-505 (1992).
26. Gupta, V. et al. Genetic transformation of Brassica nigra by Agrobacterium-based vector and direct plasmid uptake. Plant Cell Rep. 12, 418-421 (1993).
27. Damgaard, O. et al. Agrobacterium-tumefaciens-mediated transformation of Brassica napus winter cultivars. Transgenic Res. 6, 279-283 (1997).
28. Wang, W.C. et al. Development of a novel Agrobacterium mediated transformation method to recover transgerric Brassica napus plants. Plant Cell Rep. 22, 274-281 (2003).
29. Kazan, K., Curtis, M.D., Goulter, K.C. & Manners, J.M. Agrobacterium tumefaciens-mediated transformation of double haploid canola (Brassica napus) lines. Aust. J. Plant Physiol. 24, 97-102 (1997).
30. Stewart, C.N. et al. Insect control and dosage effects in transgenic canola containing a synthetic Bacillus thuringiensis crylAc gene. Plant Physiol. 112, 115-120 (1996).
31. Zhang, Y., Singh, M.B., Swoboda, I. & Bhalla, P.L. Agrobacterium mediated transformation and generation of male sterile lines of Australian canola. Aust. J. Agric. Res. 56, 353-361 (2005).
32. Bhalla, P.L. & Smith, N. Agrobacterium-mediated transformation of Australian cultivars of cauliflowers, Brassica oleracea var botrytis. Mol. Breed. 4, 531-541 (1998).
33. Bhalla, P.L. & Smith, N. Comparison of regeneration potential from seedling explants of Australian cauliflower (Brassica oleracea) varieties. Aust. J. Agric. Res. 49, 1261-1266 (1998).
34. Zhang, Y. & Bhalla, P.L In vitro shoot regeneration from commercial cultivars of Australian canola (Brassica napus L.). Aust. J. Agric. Res. 55, 753-756 (2004).
35. Murashige, T. & Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473-497 (1962).