Quantitative colocalization analysis of confocal fluorescence microscopy images

Current Protocols in Cell Biology

Volumn , Issue SUPPL. 52, 2011, Pages

  Zinchuk, Vadim ^a   Grossenbacher Zinchuk, Olga ^b  

^a KOCHI MEDICAL SCHOOL   (Japan)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

Confocal fluorescence microscopy; Image analysis; Quantitative colocalization

Indexed keywords

ARTICLE; CALCULATION; CLINICAL PROTOCOL; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; IMAGING; IMMUNOHISTOCHEMISTRY; MICROSCOPE; PRIORITY JOURNAL; QUANTITATIVE COLOCALIZATION ANALYSIS; TEST RETEST RELIABILITY; ANIMAL; CHEMISTRY; COMPUTER PROGRAM; CONFOCAL MICROSCOPY; CYTOLOGY; FLUORESCENCE; IMAGE PROCESSING; LIVER; METHODOLOGY; RAT;

ABC TRANSPORTER; ABCB11 PROTEIN, RAT; ABCC2 PROTEIN, RAT;

ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; FLUORESCENCE; IMAGE PROCESSING, COMPUTER-ASSISTED; LIVER; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; RATS; SOFTWARE;

EID: 82455192408     PISSN: 19342500     EISSN: 19342616     Source Type: Journal    
DOI: 10.1002/0471143030.cb0419s52     Document Type: Article

References (29)

1. Berg, K.A., Zardeneta, G., Hargreaves, K.M., Clarke, W.P., and Milam, S.B. 2007. Integrins regulate opioid receptor signaling in trigeminal ganglion neurons. Neuroscience 144:889-897.
2. Cario, E., Golenbock, D.T., Visintin, A., Runzi, M., Gerken, G., and Podolsky, D.K. 2006. Trypsinsensitive modulation of intestinal epithelialMD-2 asmechanism of lipopolysaccharide tolerance. J. Immunol. 176:4258-4266.
3. Clizbe, D.B., Owens, M.L., Masuda, K.R., Shacklford, J.E., and Krisans, S.K. 2007. IDI2, a second isopentenyl diphosphate isomerase in mammals. J. Biol. Chem. 282:6668-6676.
4. Criscuoli, M.L., Nguyen, M., and Eliceiri, B.P. 2005. Tumor metastasis but not tumor growth is dependent on Src-mediated vascular permeability. Blood 105:1508-1514.
5. Delputte, P.L., Van Gorp, H., Favoreel, H.W., Hoebeke, I., Delrue, I., Dewerchin, H., Verdonck, F., Verhasselt, B., Cox, E., and Nauwynck, H.J. 2011. Porcine sialoadhesin (CD169/Siglec-1) is an endocytic receptor that allows targeted delivery of toxins and antigens to macrophages. PLoS One 6:e16827.
6. Desplanques, A.S., Nauwynck, H.J., Tilleman, K., Deforce, D., and Favoreel, H.W. 2007. Tyrosine phosphorylation and lipid raft association of pseudorabies virus glycoprotein E during antibody-mediated capping. Virology 362:60-66.
7. Dombrowski, F., Stieger, B., and Beuers, U. 2006. Tauroursodeoxycholic acid inserts the bile salt export pump into canalicular membranes of cholestatic rat liver. Lab. Invest. 86:166-174.
8. Gerloff, T., Stieger, B., Hagenbuch, B., Madon, J., Landmann, L., Roth, J., Hofmann, A.F., and Meier, P.J. 1998. The sister of P-glycoprotein represents the canalicular bile salt export pump of mammalian liver. J. Biol. Chem. 273:10046-10050.
9. Head, B.P., Patel, H.H, Roth, D.M., Murray, F., Swaney, J.S., Niesman, I.R., Farquhar, M.G., and Insel, P.A. 2006. Microtubules and actin microfilaments regulate lipid raft/caveolae localization of adenylyl cyclase signaling components. J. Biol. Chem. 281:26391-26399.
10. Kato, T., Muraski, J., Chen, Y., Tsujita, Y., Wall, J., Glembotski, C.C., Schaefer, E., Beckerle, M., and Sussman, M.A. 2005. Atrial natriuretic peptide promotes cardiomyocyte survival by cGMP-dependent nuclear accumulation of zyxin and Akt. J. Clin. Invest. 115:2716-2730.
11. Konig, J., Nies, A.T., Cui, Y., Leier, I., and Keppler, D. 1999. Conjugate export pumps of the multidrug resistance protein (MRP) family: Localization, substrate specificity, and MRP2-mediated drug resistance. Biochim. Biophys. Acta 1461:377-394.
12. Landmann, L. and Marbet, P. 2004. Colocalization analysis yields superior results after image restoration. Microsc. Res. Tech. 64:103-112.
13. Lippincott-Schwartz, J., Snapp, E., and Kenworthy, A. 2001. Studying protein dynamics in living cells. Nat. Rev. Mol. Cell Biol. 2:444-456.
14. Manders, E.M.M., Verbeek, F.J., and Aten, J.A. 1993. Measurement of co-localization of objects in dual-colour confocal images. J. Microsc. 169:375-382.
15. Mutch, C.M., Sanyal, R., Unruh, T.L., Grigoriou, L., Zhu, M., Zhang, W., and Deans, J.P. 2007. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: Evidence for a role in internalization of the B cell receptor. Int. Immunol. 19:19-30.
16. North, A.J. 2006. Seeing is believing? A beginners' guide to practical pitfalls in image acquisition. J. Cell Biol. 172:9-18.
17. Patel, H.H., Head, B.P., Petersen, H.N., Niesman, I.R., Huang, D., Gross, G.J., Insel, P.A., and Roth, D.M. 2006. Protection of adult rat cardiac myocytes from ischemic cell death: Role of caveolar microdomains and delta-opioid receptors. Am. J. Physiol. Heart Circ. Physiol. 291:H344-H350.
18. Rey, O., Young, S.H., Papazyan, R., Shapiro, M.S., and Rozengurt, E. 2006. Requirement of the TRPC1 cation channel in the generation of transient Ca2+ oscillations by the calciumsensing receptor. J. Biol. Chem. 281:38730-38737.
19. Rocker, C., Manolov, D.E., Kuzmenkina, E.V., Tron, K., Slatosch, H., Torzewski, J., and Nienhaus,G.U. 2007. Affinity of C-reactive protein toward FcgammaRI is strongly enhanced by the gamma-chain. Am. J. Pathol. 170:755-763.
20. Smallcombe, A. 2001. Multicolor imaging: The important question of co-localization. Biotechniques 30:1240-1246.
21. Swaney, J.S., Patel, H.H., Yokoyama, U., Head, B.P., Roth, D.M., and Insel, P.A. 2006. Focal adhesions in (myo)fibroblasts scaffold adenylyl cyclase with phosphorylated caveolin. J. Biol. Chem. 281:17173-17179.
22. Tsutsumi, Y.M., Patel, H.H., Huang, D., and Roth, D.M. 2006. Role of 12-lipoxygenase in volatile anesthetic-induced delayed preconditioning in mice. Am. J. Physiol. Heart Circ. Physiol. 291:H979-H983.
23. Van Acker, G.J., Weiss, E., Steer, M.L., and Perides, G. 2007. Cause-effect relationships between zymogen activation and other early events in secretagogue-induced acute pancreatitis. Am. J. Physiol. Gastrointest. Liver Physiol. 292:G1738-G1746.
24. Watanabe, T., Sorensen, E.M., Naito, A., Schott, M., Kim, S., and Ahlquist, P. 2007. Involvement of host cellular multivesicular body functions in hepatitis B virus budding. Proc. Natl. Acad. Sci. U.S.A. 104:10205-10210.
25. Zinchuk, V.S., Okada, T., Akimaru, K., and Seguchi,H. 2002. Asynchronous expression and colocalization of Bsep and Mrp2 during development of rat liver. Am. J. Physiol. Gastrointest. Liver Physiol. 282:G540-G548.
26. Zinchuk, V. and Grossenbacher-Zinchuk, O. 2009. Recent advances in quantitative colocalization analysis: Focus on neuroscience. Prog. Histochem. Cytochem. 44:125-172.
27. Zinchuk, O., Fukushima, A., Hangstefer, E., and Ueno, H. 2004. Dynamics of PAF-induced conjunctivitis reveals differential expression of PAF receptor by macrophages and eosinophils in the rat. Cell Tissue Res. 317:265-277.
28. Zinchuk, O., Fukushima, A., Zinchuk, V., Fukata, K., and Ueno, H. 2005a. Direct action of platelet activating factor (PAF) induces eosinophil accumulation and enhances expression of PAF receptors in conjunctivitis. Mol. Vis. 11:114-123.
29. Zinchuk, V., Zinchuk, O., and Okada, T. 2005b. Experimental LPS-induced cholestasis alters subcellular distribution and affects colocalization of Mrp2 and Bsep proteins: A quantitative colocalization study. Microsc. Res. Tech. 67:65-70.