Asymmetric uptake of sepiapterin and 7,8-dihydrobiopterin as a gateway of the salvage pathway of tetrahydrobiopterin biosynthesis from the lumenal surface of rat endothelial cells

Molecular Genetics and Metabolism

Volumn 104, Issue 3, 2011, Pages 404-406

  Ohashi, Akiko ^a   Mamada, Kaori ^a   Tsuboi, Isao ^a   Aizawa, Shin ^a   Hasegawa, Hiroyuki ^a  

^a NIHON UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Cell polarity; Dihydrobiopterin; Endothelial cell; Nucleoside transporter; Sepiapterin; Tetrahydrobiopterin

Indexed keywords

DIHYDROBIOPTERIN; SEPIAPTERIN; TETRAHYDROBIOPTERIN;

ANIMAL CELL; ARTICLE; BIOSYNTHESIS; CELL TRANSFER; CONTROLLED STUDY; ENDOTHELIUM CELL; IMMUNOREACTIVITY; NONHUMAN; PRIORITY JOURNAL; RAT; SURFACE PROPERTY;

ANIMALS; AORTA; BIOPTERIN; ENDOTHELIAL CELLS; NITRIC OXIDE; PTERINS; RATS;

RATTUS;

EID: 82455187992     PISSN: 10967192     EISSN: 10967206     Source Type: Journal    
DOI: 10.1016/j.ymgme.2011.06.003     Document Type: Article

References (8)

1. Gross S.S., Jaffe E.A., Levi R., Kilbourn R.G. Cytokine-activated endothelial cells express an isotype of nitric oxide synthase which is tetrahydrobiopterin-dependent, calmodulin-independent and inhibited by arginine analogs with a rank-order of potency characteristic of activated macrophages. Biochem. Biophys. Res. Commun. 1991, 178:823-829.
2. Chalupsky K., Cai H. Endothelial dihydrofolate reductase: Critical for nitric oxide bioavailability and role in angiotensin II uncoupling of endothelial nitric oxide synthase. Proc. Natl. Acad. Sci. U.S.A. 2005, 102:9056-9061.
3. Ohashi A., Sugawara Y., Mamada K., Harada Y., Sumi T., Anzai N., Aizawa S., Hasegawa H. Membrane transport of sepiapterin and dihydrobiopterin by equilibrative nucleoside transporters: A plausible gateway for the salvage pathway of tetrahydrobiopterin biosynthesis. Mol. Genet. Metab. 2011, 102:18-28.
4. Vasquez-Vivar J., Martasek P., Whitsett J., Joseph J., Kalyanaraman B. The ratio between tetrahydrobiopterin and oxidized tetrahydrobiopterin analogues controls superoxide release from endothelial nitric oxide synthase: an EPR spin trapping study. Biochem. J. 2002, 362:733-739.
5. Sawabe K., Yamamoto K., Harada Y., Ohashi A., Sugawara Y., Matsuoka H., Hasegawa H. Cellular uptake of sepiapterin and push-pull accumulation of tetrahydrobiopterin. Mol. Genet. Metab. 2008, 94:410-416.
6. Santaguida S., Janigro D., Hossain M., Oby E., Rapp E., Cucullo L. Side by side comparison between dynamic versus static models of blood-brain barrier in vitro: A permeability study. Brain Res. 2006, 1109:1-13.
7. Ohashi A., Fukumuro M., Sawabe K., Mamada K., Sugawara Y., Matsuoka H., Hasegawa H. Transcellular relocation of tetrahydrobiopterin across Caco-2 cells: A model study of tetrahydrobiopterin absorption through epithelial cells of intestinal mucosa. J. Inherit. Metab. Dis. 2008, 32:73-78.
8. Poller B., Gutmann H., Krahenbuhl S., Weksler B., Romero I., Couraud P.O., Tuffin G., Drewe J., Huwyler J. The human brain endothelial cell line hCMEC/D3 as a human blood-brain barrier model for drug transport studies. J. Neurochem. 2008, 107:1358-1368.