Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells

BMC Cell Biology

Volumn 12, Issue , 2011, Pages

  Beach, Jordan R ^a,b   Licate, Lucila S ^a   Crish, James F ^a   Egelhoff, Thomas T ^a  

^a LERNER RESEARCH INSTITUTE   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; ASPARTIC ACID; MYOSIN II; SERINE; SERINE 1; SERINE 2; THREONINE; THREONINE 9; UNCLASSIFIED DRUG; MYOSIN LIGHT CHAIN;

AMINO ACID SUBSTITUTION; ARTICLE; CELL DIVISION; CELL SPREADING; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME REGULATION; HUMAN; HUMAN CELL; LIGHT CHAIN; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; CELL CULTURE; CHEMISTRY; ENZYME ACTIVE SITE; HELA CELL; METABOLISM; PHOSPHORYLATION;

CATALYTIC DOMAIN; CELL DIVISION; CELLS, CULTURED; HELA CELLS; HUMANS; MYOSIN LIGHT CHAINS; MYOSIN TYPE II; PHOSPHORYLATION; SERINE; THREONINE;

EID: 82455168277     PISSN: None     EISSN: 14712121     Source Type: Journal    
DOI: 10.1186/1471-2121-12-52     Document Type: Article

References (24)

1. Vicente-Manzanares M, Ma X, Adelstein RS, Horwitz AR. Non-muscle myosin II takes centre stage in cell adhesion and migration. Nat Rev Mol Cell Biol 2009, 10:778-790. 10.1038/nrm2786, 2834236, 19851336.
2. Adelstein RS, Conti MA. Phosphorylation of platelet myosin increases actin-activated myosin. ATPase activity Nature 1975, 256:597-598.
3. Scholey JM, Taylor KA, Kendrick-Jones J. Regulation of non-muscle myosin assembly by calmodulin-dependent light chain kinase. Nature 1980, 287:233-235. 10.1038/287233a0, 6893621.
4. Ikebe M, Hartshorne DJ, Elzinga M. Phosphorylation of the 20,000-dalton light chain of smooth muscle myosin by the calcium-activated, phospholipid-dependent protein kinase. Phosphorylation sites and effects of phosphorylation. J Biol Chem 1987, 262:9569-9573.
5. Nishikawa M, Tanaka T, Hidaka H. Ca2+-calmodulin-dependent phosphorylation and platelet secretion. Nature 1980, 287:863-865. 10.1038/287863a0, 7432502.
6. Nishikawa M, Sellers JR, Adelstein RS, Hidaka H. Protein kinase C modulates in vitro phosphorylation of the smooth muscle heavy meromyosin by myosin light chain kinase. J Biol Chem 1984, 259:8808-8814.
7. Turbedsky K, Pollard TD, Bresnick AR. A subset of protein kinase C phosphorylation sites on the myosin II regulatory light chain inhibits phosphorylation by myosin light chain kinase. Biochemistry 1997, 36:2063-2067. 10.1021/bi9624651, 9047304.
8. Yamakita Y, Yamashiro S, Matsumura F. In vivo phosphorylation of regulatory light chain of myosin II during mitosis of cultured cells. J Cell Biol 1994, 124:129-137. 10.1083/jcb.124.1.129, 2119899, 8294496.
9. Komatsu S, Ikebe M. The phosphorylation of myosin II at the Ser1 and Ser2 is critical for normal platelet-derived growth factor induced reorganization of myosin filaments. Mol Biol Cell 2007, 18:5081-5090. 10.1091/mbc.E06-12-1076, 2096596, 17928407.
10. Shuster CB, Burgess DR. Parameters that specify the timing of cytokinesis. J Cell Biol 1999, 146:981-992. 10.1083/jcb.146.5.981, 2169486, 10477753.
11. Yumura S, Yoshida M, Betapudi V, Licate LS, Iwadate Y, Nagasaki A, Uyeda TQ, Egelhoff TT. Multiple myosin II heavy chain kinases: roles in filament assembly control and proper cytokinesis in Dictyostelium. Mol Biol Cell 2005, 16:4256-4266. 10.1091/mbc.E05-03-0219, 1196335, 15987738.
12. Asano S, Hamao K, Hosoya H. Direct evidence for roles of phosphorylated regulatory light chain of myosin II in furrow ingression during cytokinesis in HeLa cells. Genes Cells 2009, 14:555-568. 10.1111/j.1365-2443.2009.01288.x, 19371382.
13. Betapudi V, Licate LS, Egelhoff TT. Distinct roles of nonmuscle myosin II isoforms in the regulation of MDA-MB-231 breast cancer cell spreading and migration. Cancer Res 2006, 66:4725-4733. 10.1158/0008-5472.CAN-05-4236, 16651425.
14. Kengyel A, Wolf WA, Chisholm RL, Sellers JR. Nonmuscle myosin IIA with a GFP fused to the N-terminus of the regulatory light chain is regulated normally. J Muscle Res Cell Motil 2010, 31:163-170. 10.1007/s10974-010-9220-y, 20711642.
15. Yamashiro S, Totsukawa G, Yamakita Y, Sasaki Y, Madaule P, Ishizaki T, Narumiya S, Matsumura F. Citron kinase, a Rho-dependent kinase, induces di-phosphorylation of regulatory light chain of myosin II. Mol Biol Cell 2003, 14:1745-1756. 10.1091/mbc.E02-07-0427, 165073, 12802051.
16. Amano M, Ito M, Kimura K, Fukata Y, Chihara K, Nakano T, Matsuura Y, Kaibuchi K. Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase). J Biol Chem 1996, 271:20246-20249. 10.1074/jbc.271.34.20246, 8702756.
17. Murata-Hori M, Suizu F, Iwasaki T, Kikuchi A, Hosoya H. ZIP kinase identified as a novel myosin regulatory light chain kinase in HeLa cells. FEBS Lett 1999, 451:81-84. 10.1016/S0014-5793(99)00550-5, 10356987.
18. Matsumura F, Hartshorne DJ. Myosin phosphatase target subunit: Many roles in cell function. Biochem Biophys Res Commun 2008, 369:149-156. 10.1016/j.bbrc.2007.12.090, 2367208, 18155661.
19. Breckenridge MT, Dulyaninova NG, Egelhoff TT. Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells. Mol Biol Cell 2009, 20:338-347. 10.1091/mbc.E08-04-0372, 2613126, 18971378.
20. Dulyaninova NG, House RP, Betapudi V, Bresnick AR. Myosin-IIA heavy-chain phosphorylation regulates the motility of MDA-MB-231 carcinoma cells. Mol Biol Cell 2007, 18:3144-3155. 10.1091/mbc.E06-11-1056, 1949358, 17567956.
21. Li ZH, Spektor A, Varlamova O, Bresnick AR. Mts1 regulates the assembly of nonmuscle myosin-IIA. Biochemistry 2003, 42:14258-14266. 10.1021/bi0354379, 14640694.
22. Welter JF, Crish JF, Agarwal C, Eckert RL. Fos-related antigen (Fra-1), junB, and junD activate human involucrin promoter transcription by binding to proximal and distal AP1 sites to mediate phorbol ester effects on promoter activity. J Biol Chem 1995, 270:12614-12622. 10.1074/jbc.270.21.12614, 7759510.
23. Wei Q, Adelstein RS. Conditional expression of a truncated fragment of nonmuscle myosin II-A alters cell shape but not cytokinesis in HeLa cells. Mol Biol Cell 2000, 11:3617-3627. 15019, 11029059.
24. Beach JR, Egelhoff TT. Myosin II recruitment during cytokinesis independent of centralspindlin-mediated phosphorylation. J Biol Chem 2009, 284:27377-27383. 10.1074/jbc.M109.028316, 2785666, 19661065.