Exon 4-skipping GLT-1: A new form of an abundantly expressed glutamate transporter

Neuroscience Letters

Volumn 504, Issue 3, 2011, Pages 228-231

  Lee, Aven ^a   Anderson, Ashley R ^a   Stevens, Melissa G ^a   Pow, David V ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Astrocyte; EAAT; Splice variant

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; EXCITATORY AMINO ACID TRANSPORTER 2; MESSENGER RNA; VESICULAR GLUTAMATE TRANSPORTER 1;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ASTROCYTE; BRAIN CELL; CELL CULTURE; CLONE; CONTROLLED STUDY; DNA ISOLATION; EXON SKIPPING; GENETIC CODE; GRAY MATTER; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TESTIS CELL; WESTERN BLOTTING;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; ASTROCYTES; BRAIN CHEMISTRY; DNA, COMPLEMENTARY; EXCITATORY AMINO ACID TRANSPORTER 2; EXONS; GLUTAMIC ACID; MALE; MOLECULAR SEQUENCE DATA; NERVE TISSUE PROTEINS; ORGAN SPECIFICITY; PROTEIN ISOFORMS; RATS; RETINA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TESTIS; VISCERA;

EID: 82455167997     PISSN: 03043940     EISSN: 18727972     Source Type: Journal    
DOI: 10.1016/j.neulet.2011.09.029     Document Type: Article

References (24)

1. Beart P.M., O'Shea R.D. Transporters for l-glutamate: an update on their molecular pharmacology and pathological involvement. Br. J. Pharmacol. 2007, 150:5-17.
2. Chen Z. Simple modifications to increase specificity of the 5'-RACE procedure. Trends Genet. 1996, 12:87-88.
3. Chen W., Mahadomrongkul V., Berger U.V., Bassan M., DeSilva T., Tanaka K., Irwin N., Aoki C., Rosenberg P.A. The glutamate transporter GLT1a is expressed in excitatory axon terminals of mature hippocampal neurons. J. Neurosci. 2004, 24:1136-1148.
4. Danbolt N.C. Glutamate uptake. Prog. Neurobiol. 2001, 65:1-105.
5. Dowd L.A., Coyle A.J., Rothstein J.D., Pritchett D.B., Robinson M.B. Comparison of Na+-dependent glutamate transport activity in synaptosomes, C6 glioma, and Xenopus oocytes expressing excitatory amino acid carrier 1 (EAAC1). Mol. Pharmacol. 1996, 49:465-473.
6. Grunewald M., Bendahan M., Kanner B. Biotinylation of single cysteine mutants of the glutamate transporter GLT-1 from rat brain reveals its unusual topology. Neuron 1998, 21:623-632.
7. Holmseth S., Scott H.A., Real K., Lehre K.P., Leergaard T.B., Bjaalie J.G., Danbolt N.C. The concentrations and distributions of three C-terminal variants of the GLT1 (EAAT2; slc1a2) glutamate transporter protein in rat brain tissue suggest differential regulation. Neuroscience 2009, 162:1055-1071.
8. Lee A., Pow D.V. Astrocytes: glutamate transport and alternate splicing of transporters. Int. J. Biochem. Cell Biol. 2010, 42:1901-1916.
9. Lee A., Anderson A.R., Barnett A.C., Chan A., Pow D.V., Anderson A.R., Barnett A.C., Chan A., Pow D.V. Expression of multiple glutamate transporter splice variants in the rodent testis. Asian J. Androl. 2011, 13:254-265.
10. Macnab L.T., Pow D.V. Expression of the exon 9-skipping form of EAAT2 in astrocytes of rats. Neuroscience 2007, 150:705-711.
11. Peacey E., Miller C.C., Dunlop J., Rattray M. The four major N- and C-terminal splice variants of the excitatory amino acid transporter GLT-1 form cell surface homomeric and heteromeric assemblies. Mol. Pharmacol. 2009, 75:1062-1073.
12. Pines G., Danbolt N., Bjørås M., Zhang Y., Bendahan A., Eide L., Koepsell H., Storm-Mathisen J., Seeberg E., Kanner B.I. Cloning and expression of a rat brain l-glutamate transporter. Nature 1992, 360:464-467.
13. Pow D.V. Amino acids and their transporters in the retina. Neurochem. Int. 2001, 38:463-484.
14. Pow D.V., Sullivan R., Scott H. Antibody production and immunocytochemical localization of amino acid transporters. Methods Mol. Biol. 2003, 227:213-244.
15. Pow D.V., Naidoo T., Lingwood B.E., Healy G.N., Williams S.M., Sullivan R.K., O'Driscoll S., Colditz P.B. Loss of glial glutamate transporters and induction of neuronal expression of GLT-1B in the hypoxic neonatal pig brain. Brain Res. Dev. Brain Res. 2004, 153:1-11.
16. Rauen T., Wiessner M., Sullivan R., Lee A., Pow D.V. A new GLT1 splice variant: cloning and immunolocalization of GLT1c in the mammalian retina and brain. Neurochem. Int. 2004, 45:1095-1106.
17. Reye P., Sullivan R., Fletcher E.L., Pow D.V. Distribution of two splice variants of the glutamate transporter GLT-1 in the retinas of humans, monkeys, rabbits, rats, cats, and chickens. J. Comp. Neurol. 2002, 445:1-12.
18. Reye P., Sullivan R., Scott H., Pow D.V. Distribution of two splice variants of the glutamate transporter GLT-1 in rat brain and pituitary. Glia 2002, 38:246-255.
19. Reye P., Sullivan R., Pow D.V. Distribution of two splice variants of the glutamate transporter GLT-1 in the developing rat retina. J. Comp. Neurol. 2002, 447:323-330.
20. Robinson M.B. The family of sodium-dependent glutamate transporters: a focus on the GLT-1/EAAT2 subtype. Neurochem. Int. 1998, 33:479-491.
21. Rothstein J.D., Dykes-Hoberg M., Pardo C.A., Bristol L.A., Jin L., Kuncl R.W., Kanai Y., Hediger M.A., Wang Y., Schielke J.P., Welty D.F. Knockout of glutamate transporters reveals a major role for astroglial transport in excitotoxicity and clearance of glutamate. Neuron 1996, 16:675-686.
22. Tian G., Lai L., Guo H., Lin Y., Butchbach M.E., Chang Y., Lin C.L. Translational control of glial glutamate transporter EAAT2 expression. J. Biol. Chem. 2007, 282:1727-1737.
23. Vallejo-Illarramendi A., Domercq M., Matute C. A novel alternative splicing form of excitatory amino acid transporter 1 is a negative regulator of glutamate uptake. J. Neurochem. 2005, 95:341-348.
24. Williams S.M., Sullivan R.K., Scott H.L., Finkelstein D.I., Colditz P.B., Lingwood B.E., Dodd P.R., Pow D.V. Glial glutamate transporter expression patterns in brains from multiple mammalian species. Glia 2005, 49:520-541.