Intracellular expression of Vitreoscilla hemoglobin improves production of Yarrowia lipolytica lipase LIP2 in a recombinant Pichia pastoris

Enzyme and Microbial Technology

Volumn 50, Issue 1, 2012, Pages 22-28

  Wang, Xiaofeng ^a   Sun, Yongchuan ^a   Shen, Xuguang ^a   Ke, Feng ^a   Zhao, Heyun ^a   Liu, Yun ^a   Xu, Li ^a   Yan, Yunjun ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Expression; Lipase; Pichia pastoris; Vitreoscilla hemoglobin; Yarrowia lipolytica

Indexed keywords

CELL VIABILITY; CONTROL STRAIN; EXPRESSION; EXPRESSION LEVELS; FERMENTORS; INDUSTRIAL ENZYMES; LIMITING FACTORS; OXYGEN LIMITATION; P. PASTORIS; PICHIA PASTORIS; RECOMBINANT STRAINS; SHAKE-FLASK CULTURES; SPECIFIC OXYGEN UPTAKE RATE; VGB GENES; VITREOSCILLA HEMOGLOBIN; VITREOSCILLA HEMOGLOBIN GENE; YARROWIA LIPOLYTICA;

CELL CULTURE; CELLS; FERMENTERS; GENE EXPRESSION; HEMOGLOBIN; LIPASES; STRAIN; YEAST;

DISSOLVED OXYGEN;

DISSOLVED OXYGEN; HEMOGLOBIN; LIPASE 2; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ARTICLE; BIOREACTOR; CELL DENSITY; CELL GROWTH; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SYNTHESIS; FED BATCH FERMENTATION; FUNGAL BIOMASS; GENETIC ENGINEERING; MICROBIAL RESPIRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OXYGEN CONSUMPTION; PICHIA PASTORIS; PROMOTER REGION; PROTEIN EXPRESSION; VITREOSCILLA; YARROWIA LIPOLYTICA;

BACTERIAL PROTEINS; BASE SEQUENCE; BIOREACTORS; DNA PRIMERS; FERMENTATION; FUNGAL PROTEINS; GENE EXPRESSION; GENES, BACTERIAL; GENES, FUNGAL; LIPASE; PICHIA; RECOMBINANT PROTEINS; TRUNCATED HEMOGLOBINS; VITREOSCILLA; YARROWIA;

PICHIA PASTORIS; VITREOSCILLA; YARROWIA LIPOLYTICA;

EID: 82455167789     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2011.09.003     Document Type: Article

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