Two high linolenic mutants of Arabidopsis thaliana contain megabase-scale genome duplications encompassing the FAD3 locus

Plant Journal

Volumn 68, Issue 5, 2011, Pages 912-918

  O'Neill, Carmel M ^a   Baker, David ^b   Bennett, Gawain ^b   Clarke, Jonathan ^a   Bancroft, Ian ^a  

^a JOHN INNES CENTRE   (United Kingdom)

^b EARLHAM INSTITUTE   (United Kingdom)

Author keywords

fast neutron; ife; oil biosynthesis; polyunsaturated fatty acid; rfc4; T DNA

Indexed keywords

ARABIDOPSIS THALIANA; DESATURASE; DESATURATION; FAST NEUTRONS; GENOME DUPLICATION; GENOME STRUCTURES; GENOMIC REGIONS; GERMPLASMS; HUMAN HEALTH; IFE; LINOLENIC; LINOLENIC ACIDS; OIL CROPS; OMEGA-3; ORTHOLOGUES; RFC4; SCREENING METHODS; SEED OIL; SEED STORAGE; T-DNA;

BIOCHEMISTRY; BIOSYNTHESIS; DNA; GENES; INDUSTRIAL APPLICATIONS; INDUSTRIAL RESEARCH; MINERAL OILS; NEUTRONS; OILS AND FATS; PLANTS (BOTANY); POLYUNSATURATED FATTY ACIDS; SEED;

FATTY ACIDS;

ACYL COENZYME A DESATURASE; ARABIDOPSIS PROTEIN; FAD3 PROTEIN, ARABIDOPSIS; LINOLENIC ACID; PLANT DNA; SEED STORAGE PROTEIN; VEGETABLE OIL;

ARABIDOPSIS; ARTICLE; CHROMOSOME DUPLICATION; CHROMOSOME MAP; CROSS BREEDING; DNA SEQUENCE; FAST NEUTRON RADIATION; GENE EXPRESSION REGULATION; GENE INACTIVATION; GENE LOCUS; GENETICS; METABOLISM; MOLECULAR CLONING; PLANT CHROMOSOME; PLANT GENE; PLANT GENOME; PLANT SEED; PROMOTER REGION; TRANSGENIC PLANT;

ALPHA-LINOLENIC ACID; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CHROMOSOME DUPLICATION; CHROMOSOME MAPPING; CHROMOSOMES, PLANT; CLONING, MOLECULAR; CROSSES, GENETIC; DNA, PLANT; FAST NEUTRONS; FATTY ACID DESATURASES; GENE KNOCKOUT TECHNIQUES; GENES, PLANT; GENETIC LOCI; GENOME, PLANT; MUTAGENESIS, INSERTIONAL; PLANT OILS; PLANTS, GENETICALLY MODIFIED; PROMOTER REGIONS, GENETIC; SEED STORAGE PROTEINS; SEEDS; SEQUENCE ANALYSIS, DNA;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 82355190267     PISSN: 09607412     EISSN: 1365313X     Source Type: Journal    
DOI: 10.1111/j.1365-313X.2011.04742.x     Document Type: Article

References (36)

1. Bancroft, I., Jones, J.D.G., and, Dean, C., (1993) Heterologous transposon tagging of the DRL1 locus in Arabidopsis. Plant Cell, 5, 631-638.
2. Bates, P.D., Durrett, T.P., Ohlrogge, J.B., and, Pollard, M., (2009) Analysis of acyl fluxes through multiple pathways of triacylglycerol synthesis in developing soybean embryos. Plant Physiol. 150, 55-72.
3. Bent, E., Johnson, S., and, Bancroft, I., (1998) BAC representation of two low-copy regions of the genome of Arabidopsis thaliana. Plant J. 13, 849-855. (Pubitemid 28178646)
4. Browse, J., McConn, M., James, D., and, Miquel, M., (1993) Mutants of Arabidopsis deficient in the synthesis of alpha-linolenate-biochemical and genetic-characterization of the endoplasmic-reticulum linoleoyl desaturase. J Biol Chem. 268, 16345-16351. (Pubitemid 23229937)
5. Colbert, T., Till, B.J., Tompa, R., Reynolds, S., Steine, M.N., Yeung, A.T., McCallum, C.M., Comai, L., and, Henikoff, S., (2001) High-throughput screening for induced point mutations. Plant Physiol. 126, 480-484. (Pubitemid 32566603)
6. Feldmann, K.A., and, Marks, M.D., (1987) Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana: a non-tissue culture approach. Mol.Gen.Genet. 208, 1-9.
7. Gheysen, G., Villarroel, R., and, Van Montagu, M., (1991) Illegitimate recombination in plants: a model for T-DNA integration. Genes Dev. 5, 287-297. (Pubitemid 21905953)
8. Greene, E.A., Codomo, C.A., Taylor, N.E., et al. (2003) Spectrum of chemically induced mutations from a large-scale Reverse-Genetic screen in Arabidopsis. Genetics, 164, 731-740. (Pubitemid 36773508)
9. Hobbs, D.H., Lu, C., and, Hills, M.J., (1999) Cloning of a cDNA encoding diacylglycerol acyltransferase from Arabidopsis thaliana and its functional expression. FEBS Lett. 452, 145-149. (Pubitemid 29301734)
10. Horiguchi, G., Fuse, T., Kawakami, N., Kodama, H., and, Iba, K., (2000) Temperature-dependent translational regulation of the ER ω-3 fatty acid desaturase gene in wheat root tips. Plant J. 24, 805-813. (Pubitemid 32100478)
11. Horiguchi, G., Kodama, H., and, Iba, K., (2001) Characterization of Arabidopsis mutants that are associated with altered C18 unsaturated fatty acid metabolism. Plant Sci. 161, 1117-1123. (Pubitemid 34003147)
12. James, D.W., and, Dooner, H.K., (1990) Isolation of EMS-induced mutants in Arabidopsis altered in seed fatty acid composition. Theor. Appl. Genet. 80, 241-245.
13. Kachroo, P., Shanklin, J., Shah, J., Whittle, E.J., and, Klessig, D.F., (2001) A fatty acid desaturase modulates the activation of defense signaling pathways in plants. Proc. Natl Acad. Sci. USA, 98, 9448-9453. (Pubitemid 32743937)
14. Koncz, C., Németh, K., Redei, G.P., and, Schell, J., (1992) T-DNA insertional mutagenesis in Arabidopsis. Plant Mol. Biol. 20, 963-976.
15. Koncz, C., Németh, K., Redei, G.P., and, Schell, J., (1994) Homology recognition during T-DNA integration into the plant genome. In Homologous Recombination and Gene Silencing in Plants (, Paszkowski, J., ed). Dordrecht, The Netherlands: Kluwer Academic Publishers, pp. 167-189.
16. Koornneef, M., Dellaert, L.W.M., and, van der Veen, J.H., (1982) EMS- and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutat. Res. 93, 109-123. (Pubitemid 12171330)
17. Lemieux, B., Miquel, M., Somerville, C., and, Browse, J., (1990) Mutants of Arabidopsis with alterations in seed lipid fatty acid composition. Theor. Appl. Genet. 80, 234-240.
18. Li, X., Song, Y., Century, K., Straight, S., Ronald, P., Dong, X., Lassner, M., and, Zhang, Y., (2001) A fast neutron deletion mutagenesis-based reverse genetics system for plants. Plant J. 27, 235-242. (Pubitemid 32778814)
19. Lu, C., Xina, Z., Zhonghai Ren, Z., Miquel, M., and, Browse, J., (2009) An enzyme regulating triacylglycerol composition is encoded by the ROD1 gene of Arabidopsis. Proc. Natl Acad. Sci. USA, 106, 18837-18842.
20. Mayerhofer, R., Koncz-Kalman, Z., Nawrath, C., Bakkeren, G., Crameri, A., Angelis, K., Redei, G.P., Schell, J., Hohn, B., and, Koncz, C., (1991) T-DNA integration: a mode of illegitimate recombination in plants. EMBO J. 10, 697-704. (Pubitemid 21905525)
21. McClintock, B., (1984) The significance of responses of the genome to challenge. Science, 226, 792-801. (Pubitemid 15200674)
22. Miquel, M., and, Browse, J., (1992) Arabidopsis mutants deficient in polyunsaturated fatty-acid synthesis-biochemical and genetic-characterization of a plant oleoyl-phosphatidylcholine desaturase. J. Biol. Chem. 267, 1502-1509.
23. Nacry, P., Camilleri, C., Courtial, B., Caboche, M., and, Bouchez, D., (1998) Major chromosomal rearrangements induced by T-DNA transformation in Arabidopsis. Genetics, 149, 641-650. (Pubitemid 28283148)
24. Ohlrogge, J., and, Browse, J., (1995) Lipid biosynthesis. Plant Cell, 7, 957-970.
25. Okuley, J., Lightner, J., Feldman, K., Yadav, N., Lark, E., and, Browse, J., (1994) Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell, 6, 147-158.
26. O'Neill, C.M., and, Bancroft, I., (2000) Comparative physical mapping of segments of the genome of Brassica oleracea var. alboglabra that are homoeologous to sequenced regions of chromosomes 4 and 5 of Arabidopsis thaliana. Plant J. 23, 233-243. (Pubitemid 30597283)
27. O'Neill, C.M., Gill, S., Hobbs, D., Morgan, C., and, Bancroft, I., (2003) Natural variation for seed oil composition in Arabidopsis thaliana. Phytochemistry, 64, 1077-1090. (Pubitemid 37295330)
28. Oomen, C.M., Ocké, M.C., Feskens, E.J.M., van Erp-Baart, M.A.J., Kok, F.J., and, Kromhout, D., (2001) Association between trans fatty acid intake and 10-year risk of coronary heart disease in the Zutphen Elderly Study: a prospective population-based study. Lancet, 357, 746-751. (Pubitemid 32217681)
29. O'Quin, J.B., Bourassa, L., Zhang, D., Shockey, J.M., Gidda, S.K., Fosnot, S., Chapman, K.D., Mullen, R.T., and, Dyer, J.M., (2010) Temperature-sensitive post-translational regulation of plant omega-3 fatty-acid desaturases is mediated by the endoplasmic reticulum-associated degradation pathway. J. Biol. Chem. 285, 21781-21796.
30. Routaboul, J.M., Benning, C., Bechtold, N., Caboche, M., and, Lepiniec, L., (1999) The TAG1 locus of Arabidopsis encodes for a diacylglycerol acyltransferase. Plant Physiol. Biochem. 37, 831-840. (Pubitemid 30001054)
31. Scheffler, J.A., Sharpe, A.G., Schmidt, H., Sperling, P., Parkin, I.A.P., Lühs, W., Lydiate, D.J., and, Heinz, E., (1997) Desaturase multigene families of Brassica napus arose through genome duplication. Theor. Appl. Genet. 9, 583-591. (Pubitemid 27201450)
32. Shah, S., Xin, Z., and, Browse, J., (1997) Overexpression of the FAD3 desaturase gene in a mutant of Arabidopsis. Plant Physiol. 114, 1533-1539. (Pubitemid 28382315)
33. Shirley, B.W., Hanley, S., and, Goodman, H.M., (1992) Effects of ionizing radiation on a plant genome: analysis of two Arabidopsis transparent testa mutations. Plant Cell, 4, 333-347.
34. Sun, T.P., Goodman, H.M., and, Ausubel, F.M., (1992) Cloning the Arabidopsis GA1 locus by genomic subtraction. Plant Cell, 4, 119-128.
35. Tax, F.E., and, Vernon, D.M., (2001) T-DNA-associated duplication/translocations in Arabidopsis. Implications for mutant analysis and functional genomics. Plant Physiol. 126, 1527-1538. (Pubitemid 32752381)
36. Zambryski, P., Tempé, J., and, Schell, J., (1989) Transfer and function of T-DNA genes from Agrobacterium Ti and Ri plasmids in plants. Cell, 56, 193-201.