High-yield expression, purification, characterization, and structure determination of tag-free Candida utilis uricase

Applied Microbiology and Biotechnology

Volumn 92, Issue 3, 2011, Pages 529-537

  Liu, Xiaojuan ^a   Wen, Mingjie ^a   Li, Jing ^b   Zhai, Fangli ^a   Ruan, Jing ^a   Zhang, Liqing ^a   Li, Shentao ^a  

^a CAPITAL MEDICAL UNIVERSITY   (China)

^b CAPITAL NORMAL UNIVERSITY   (China)

Author keywords

Candida utilis; Characterization; Crystal structure; Prokaryotic expression; Uricase

Indexed keywords

CANDIDA UTILIS; MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME-OF-FLIGHT; PROKARYOTIC EXPRESSION; PROTEIN DATABASE; PROTEIN PURIFICATION; RECOMBINANT PROTEIN; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; SPACE GROUPS; SPECIFIC ACTIVITY; STRUCTURE DETERMINATION; THREE-DIMENSIONAL STRUCTURE; UNIT CELL PARAMETERS; URICASE;

CANDIDA; CATALYST ACTIVITY; CHARACTERIZATION; CRYSTALS; DESORPTION; ELECTROPHORESIS; ESCHERICHIA COLI; GLYCOLS; MASS SPECTROMETERS; POLYETHYLENE GLYCOLS; PROTEINS; PURIFICATION; SODIUM; SODIUM SULFATE; YEAST;

CRYSTAL STRUCTURE;

BACTERIAL ENZYME; URATE OXIDASE;

CATALYSIS; COLIFORM BACTERIUM; CRYSTAL STRUCTURE; DATABASE; DESORPTION; DIFFRACTION; ELECTROKINESIS; GEL; GENE EXPRESSION; HOMOLOGY; IONIZATION; MASS SPECTROMETRY; MATRIX; ORGANIC COMPOUND; PROTEIN; RECOMBINATION; RESOLUTION; YEAST;

ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; CRYSTALLIZATION; DIFFRACTION; ENZYME ACTIVITY; ESCHERICHIA COLI; GEL ELECTROPHORESIS; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA JADINII; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

CANDIDA; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FUNGAL PROTEINS; GENE EXPRESSION; MODELS, MOLECULAR; PROTEIN STRUCTURE, QUATERNARY; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; URATE OXIDASE;

ESCHERICHIA COLI; PICHIA JADINII; PROKARYOTA;

EID: 82355186708     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3244-0     Document Type: Article

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