Overexpression and biochemical characterization of DagA from Streptomyces coelicolor A3(2): An endo-type β-agarase producing neoagarotetraose and neoagarohexaose

Applied Microbiology and Biotechnology

Volumn 92, Issue 4, 2011, Pages 749-759

  Temuujin, Uyangaa ^a   Chi, Won Jae ^a   Lee, Soon Youl ^b   Chang, Yong Keun ^c   Hong, Soon Kwang ^a  

^a Myong Ji University   (South Korea)

^b Hankyong National University   (South Korea)

^c Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Agarase; DagA; Neoagarohexaose; Neoagarotetraose; Streptomyces coelicolor

Indexed keywords

AGAROSE; AGAROSE BEADS; BIOCHEMICAL CHARACTERIZATION; DAGA; ENZYMATIC PROPERTIES; EXPRESSION SYSTEM; HYDROLYZED PRODUCTS; MALDI-TOF; MATRIX ASSISTED LASER DESORPTION; NEOAGAROHEXAOSE; NEOAGAROTETRAOSE; OVER-EXPRESSION; SIGNAL PEPTIDE; SOLE CARBON SOURCE; STREPTOMYCES COELICOLOR; STREPTOMYCES COELICOLOR A3(2); STREPTOMYCES LIVIDANS; TRANSFORMANTS;

AMINO ACIDS; COLUMN CHROMATOGRAPHY; DESORPTION; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE; PURIFICATION; THIN LAYER CHROMATOGRAPHY;

BACTERIA;

AGAROSE; BACTERIAL PROTEIN; DAGA PROTEIN; NEOAGAROHEXAOSE; NEOAGAROTETRAOSE; UNCLASSIFIED DRUG;

AGAR; AMINO ACID; BACTERIUM; BIOCHEMICAL COMPOSITION; BIOLOGICAL PRODUCTION; BIOTRANSFORMATION; CARBON; CHROMATOGRAPHY; DESORPTION; ENZYME ACTIVITY; FTIR SPECTROSCOPY; GENE EXPRESSION; IONIZATION; MASS SPECTROMETRY; MATRIX; PEPTIDE; PROTEIN;

ARTICLE; BACTERIAL STRAIN; CARBON SOURCE; COLUMN CHROMATOGRAPHY; ENZYME KINETICS; FOURIER TRANSFORM MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PH; STREPTOMYCES COELICOLOR; STREPTOMYCES LIVIDANS; THIN LAYER CHROMATOGRAPHY;

BACTERIAL PROTEINS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, THIN LAYER; CULTURE MEDIA; GALACTOSIDES; GENE EXPRESSION; GLYCOSIDE HYDROLASES; HYDROGEN-ION CONCENTRATION; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR WEIGHT; OLIGOSACCHARIDES; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STREPTOMYCES COELICOLOR; STREPTOMYCES LIVIDANS; TEMPERATURE;

PTEROGYNE NITENS; STREPTOMYCES; STREPTOMYCES COELICOLOR; STREPTOMYCES LIVIDANS;

EID: 82355186063     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3347-7     Document Type: Article

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