Expression, purification and preliminary X-ray diffraction analysis of the catalytic module of a β - AAgarase from the flavobacterium Zobellia galactanivorans

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 66, Issue 4, 2010, Pages 413-417

  Hehemann, Jan Hendrik ^a,b   Michel, Gurvan ^a,b   Barbeyron, Tristan ^a,b   Czjzek, Mirjam ^a,b  

^a SORBONNE UNIVERSITÉ   (France)

^b CNRS   (France)

Author keywords

agarases; Glycoside hydrolases; Heterologous expression; Zobellia galactanivorans

Indexed keywords

AGARASE; GLYCOSIDASE;

AMINO ACID SEQUENCE; ARTICLE; BIOCATALYSIS; CHEMISTRY; ENZYMOLOGY; FLAVOBACTERIACEAE; GENE EXPRESSION; MOLECULAR GENETICS; SEQUENCE ALIGNMENT; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BIOCATALYSIS; CRYSTALLOGRAPHY, X-RAY; FLAVOBACTERIACEAE; GENE EXPRESSION; GLYCOSIDE HYDROLASES; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT;

ALGAE; BACTERIA (MICROORGANISMS); FLAVOBACTERIUM; ZOBELLIA GALACTANIVORANS;

EID: 77950836075     PISSN: 17443091     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S174430911000429X     Document Type: Article

References (23)

1. Allouch, J., Helbert, W., Henrissat, B. & Czjzek, M. (2004). Structure, 12, 623-632.
2. Allouch, J., Jam, M., Helbert, W., Barbeyron, T., Kloareg, B., Henrissat, B. & Czjzek, M. (2003). J. Biol. Chem. 278, 47171-47180.
3. Bergfors, T. (2003). J. Struct. Biol. 142, 66-76.
4. Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760-763.
5. Collins, B. K., Tomanicek, S. J., Lyamicheva, N., Kaiser, M. W. & Mueser, T. C. (2004). Acta Cryst. D60, 1674-1678.
6. Corpet, F. (1988). Nucleic Acids Res. 16, 10881-10890.
7. Craigie, J. S. (1990). Biology of the Red Algae, edited by K. Cole & R. Sheath, pp. 221-257. Cambridge University Press.
8. Craigie, J. S. & Leigh, C. (1978). Handbook of Phycological Methods, edited by J. A. Hellebust & J. S. Craigie, pp. 109-133. Cambridge University Press.
9. Ekborg, N. A., Gonzalez, J. M., Howard, M. B., Taylor, L. E., Hutcheson, S. W. & Weiner, R. M. (2005). Int. J. Syst. Evol. Microbiol. 55, 1545-1549.
10. Ekborg, N. A., Taylor, L. E., Longmire, A. G., Henrissat, B., Weiner, R. M. & Hutcheson, S. W. (2006). Appl. Environ. Microbiol. 72, 3396-3405.
11. Gouet, P., Robert, X. & Courcelle, E. (2003). Nucleic Acids Res. 31, 3320-3323.
12. Henrissat, B. & Davies, G. (1997). Curr. Opin. Struct. Biol. 7, 637-644.
13. Jam, M., Flament, D., Allouch, J., Potin, P., Thion, L., Kloareg, B., Czjzek, M., Helbert, W., Michel, G. & Barbeyron, T. (2005). Biochem. J. 385, 703-713.
14. Knutsen, S., Myslabodski, D., Larsen, B. & Usov, A. (1994). Botanica Marina, 37, 163-169.
15. Lahaye, M. (2001). J. Appl. Phycol. 13, 173-184.
16. Leslie, A.G.W. (1992). Jnt CCP4/ESF-EACBMNewsl. Protein Crystallogr. 26.
17. Michel, G., Nyval-Collen, P., Barbeyron, T., Czjzek, M. & Helbert, W. (2006). Appl. Microbiol. Biotechnol. 71, 23-33.
18. Morrice, L. M., McLean, M.W., Long,W. F. & Williamson, F. B. (1983). Eur. J. Biochem. 133, 673-684.
19. Navaza, J. (2001). Acta Cryst. D57, 1367-1372.
20. Rees, D. A. (1969). Adv. Carbohydr. Chem. Biochem. 24, 267-332.
21. Rees, D. A. & Conway, E. (1962). Biochem. J. 84, 411-416.
22. Rochas, C., Lahaye, M. & Yaphe, W. (1986). Botanica Marina, 29, 335-340.
23. Studier, F. W. (2005). Protein Expr. Purif. 41, 207-234.