Protein Tyrosine Phosphatase Assays

Current Protocols in Immunology

Volumn , Issue SUPPL.93, 2011, Pages

  Lorenz, Ulrike ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Immunoprecipitation; Malachite green assay; Phosphopeptide; Phosphoprotein; Phosphoproteins; pNPP; Protein tyrosine phosphatase; Protein tyrosine phosphatase assay; Radioactively labeled

Indexed keywords

4 NITROPHENYL PHOSPHATE; MALACHITE GREEN; PHOSPHATE; PHOSPHOPROTEIN; PROTEIN A; PROTEIN G; PROTEIN TYROSINE KINASE; PROTEIN TYROSINE PHOSPHATASE;

ANTIGEN ANTIBODY COMPLEX; ARTICLE; CELL FRACTIONATION; CONFORMATIONAL TRANSITION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; IMMUNE SYSTEM; IMMUNOPRECIPITATION; ISOTOPE LABELING; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; PROTEIN PHOSPHORYLATION;

EID: 82355184243     PISSN: 19343671     EISSN: 1934368X     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Alonso, A., Sasin, J., Bottini, N., Friedberg, I., Osterman, A., Godzik, A., Hunter, T., Dixon, J., and Mustelin, T. 2004. Protein tyrosine phosphatases in the human genome. Cell 117:699-711.
2. Carter, S.G. and Karl, D.W. 1982. Inorganic phosphate assay with malachite green: An improvement and evaluation. J. Biochem. Biophys. Methods 7:7-13.
3. Echan, L.A. and Speicher, D.W. 2002. Protein dettection in gels using fixation. Curr. Protoc. Protein Sci. 29:10.5.1-10.5.18.
4. Gallagher, S. 2001. Immunoblot detection. Curr. Protoc. Protein Sci. 4:10.10.1-10.10.12.
5. Hermiston, M.L., Xu, Z., and Weiss, A. 2003. CD45: A critical regulator of signaling thresholds in immune cells. Annu. Rev. Immunol. 21:107-137.
6. Holmes, N. 2006. CD45: All is not yet crystal clear. Immunology 117:145-155.
7. Huntington, N.D. and Tarlinton, D.M. 2004. CD45: Direct and indirect government of immune regulation. Immunol. Lett. 94:167-174.
8. Kilgore, N.E., Carter, J.D., Lorenz, U., and Evavold, B.D. 2003. Cutting edge: Dependence of TCR antagonism on Src homology 2 domaincontaining protein tyrosine phosphatase activity. J. Immunol. 170:4891-4895.
9. Markova, B.,Gulati, P., Herrlich, P.A., and Bohmer, F.D. 2005. Investigation of protein-tyrosine phosphatases by in-gel assays. Methods 35:22-27.
10. Montalibet, J., Skorey, K.I., and Kennedy, B.P. 2005. Protein tyrosine phosphatase: Enzymatic assays. Methods 35:2-8.
11. Pao, L.I., Badour, K., Siminovitch, K.A., and Neel, B.G. 2007. Nonreceptor protein-tyrosine phosphatases in immune cell signaling. Annu. Rev. Immunol. 25:473-523.
12. Segel, I.H. 1976. Biochemical Calculations:Howto Solve Mathematical Problems in General Biochemistry. John Wiley & Sons, New York.
13. Sozio, M.S., Mathis, M.A., Young, J.A., Walchli, S., Pitcher, L.A., Wrage, P.C., Bartok, B., Campbell, A., Watts, J.D., Aebersold, R., Hooft van Huijsduijnen, R., and van Oers, N.S. 2004. PTPH1 is a predominant protein-tyrosine phosphatase capable of interacting with and dephosphorylating the T cell receptor zeta subunit. J. Biol. Chem. 279:7760-7769.
14. Tiganis,T. andBennett, A.M. 2007. Protein tyrosine phosphatase function: The substrate perspective. Biochem. J. 402:1-15.
15. Van Veldhoven, P.P. and Mannaerts, G.P. 1987. Inorganic and organic phosphate measurements in the nanomolar range. Anal. Biochem. 161:45-48.
16. Zhang, Z.Y. 1998. Protein-tyrosine phosphatases: Biological function, structural characteristics, and mechanism of catalysis. Crit. Rev. Biochem. Mol. Biol. 33:1-52.