Fluorescence measurement of mitochondrial membrane potential changes in cultured cells

Methods in Molecular Biology

Volumn 810, Issue , 2012, Pages 119-133

  Nicholls, David G ^a  

^a BUCK INSTITUTE FOR RESEARCH ON AGING   (United States)

Author keywords

Glutamate; JC 1; Membrane potential; Mitochondria; Neuron; Proton electrochemical potential; Proton motive force; Rhodamine 123; Tetramethylrhodamine methyl ester

Indexed keywords

FLUORESCENT DYE; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; ORGANOMETALLIC COMPOUND; RHODAMINE 123; TETRAMETHYL RHODAMINE ETHYL ESTER;

ANIMAL; ARTICLE; CELL CULTURE; CEREBELLUM; METABOLISM; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; NERVE CELL; PRIMARY CELL CULTURE; RAT;

ANIMALS; CELLS, CULTURED; CEREBELLUM; FLUORESCENT DYES; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA; NEURONS; ORGANOMETALLIC COMPOUNDS; PRIMARY CELL CULTURE; RATS; RECEPTORS, N-METHYL-D-ASPARTATE; RHODAMINE 123;

EID: 82355183880     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-382-0_8     Document Type: Article

References (8)

1. Nicholls DG, Ferguson SJ (2002) Bioenergetics, vol 3. Academic, London
2. Hoek JB, Nicholls DG, Williamson JR (1980) Determination of the mitochondrial protonmotive force in isolated hepatocytes. J Biol Chem 255:1458-1464
3. Ward MW, Rego AC, Frenguelli BG, Nicholls DG (2000) Mitochondrial membrane potential and glutamate excitotoxicity in cultured cerebellar granule cells. J Neurosci 20:7208-7219
4. Nicholls DG (2006) Simultaneous monitoring of ionophore- and inhibitor-mediated plasma and mitochondrial membrane potential changes in cultured neurons. J Biol Chem 281:14864-14874 (Pubitemid 43855191)
5. Ward MW, Huber HJ, Weisova P, Duessmann H, Nicholls DG, Prehn JHM (2007) Mitochondrial and plasma membrane potential of cultured cerebellar neurons during glutamate induced necrosis, apoptosis and tolerance. J Neurosci 27:8238-8249 (Pubitemid 47219629)
6. Lemasters JJ, Ramshesh VK (2007) Imaging of mitochondrial polarization and depolarization with cationic fl uorophores. Methods Cell Biol 80:283-295
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8. Rego AC, Vesce S, Nicholls DG (2001) The mechanism of mitochondrial membrane potential retention following release of cytochrome c in apoptotic GT1-7 neural cells. Cell Death Differ 8:995-1003 (Pubitemid 32994650)