Heterologous expression, refolding and functional characterization of two antifreeze proteins from Fragilariopsis cylindrus (Bacillariophyceae)

Cryobiology

Volumn 63, Issue 3, 2011, Pages 220-228

  Uhlig, Christiane ^a   Kabisch, Johannes ^b   Palm, Gottfried J ^b   Valentin, Klaus ^a   Schweder, Thomas ^b   Krell, Andreas ^a  

^a ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESEARCH   (Germany)

^b UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

Antifreeze protein; Diatom; Fragilariopsis cylindrus; Heterologous expression; Recrystallization inhibition

Indexed keywords

ANTIFREEZE PROTEIN; BUFFER; PELB LEADER PEPTIDE; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; CELLULAR DISTRIBUTION; COLD SENSITIVITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLIZATION; DIATOM; ESCHERICHIA COLI; FRAGILARIOPSIS CYLINDRUS; HETEROLOGOUS EXPRESSION; HYSTERESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN REFOLDING; PROTEIN SECRETION; SALINITY;

ANTIFREEZE PROTEINS; CLONING, MOLECULAR; COLD CLIMATE; COLD TEMPERATURE; CRYSTALLIZATION; DIATOMS; ESCHERICHIA COLI; FREEZING; ICE COVER; INCLUSION BODIES; PLASMIDS; PROTEIN ISOFORMS; PROTEIN REFOLDING; RECOMBINANT FUSION PROTEINS; SALINITY; TRANSFORMATION, BACTERIAL;

BACILLARIOPHYCEAE; BACILLARIOPHYTA; ESCHERICHIA COLI; FRAGILARIOPSIS CYLINDRUS;

EID: 82155196420     PISSN: 00112240     EISSN: 10902392     Source Type: Journal    
DOI: 10.1016/j.cryobiol.2011.08.005     Document Type: Article

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