Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

Journal of Infection in Developing Countries

Volumn 5, Issue 11, 2011, Pages 786-791

  Marei, Ayman ^a   Boghdadi, Ghada ^a   Abdel Hamed, Nahla ^a   Hessin, Rasha ^a   Abdoel, Theresia ^b   Smits, Henk ^b   Fathey, Fayza ^a  

^a Zagazig University   (Egypt)

^b ROYAL TROPICAL INSTITUTE   (Netherlands)

Author keywords

Brucellosis; Lateral flow assay; PCR; Treatment

Indexed keywords

CIPROFLOXACIN; DOXYCYCLINE; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M;

ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BLOOD SAMPLING; BRUCELLA; BRUCELLOSIS; CLINICAL ARTICLE; DIAGNOSTIC TEST ACCURACY STUDY; EGYPT; FEMALE; FOLLOW UP; HUMAN; LABORATORY DIAGNOSIS; LATERAL FLOW ASSAY; MALE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROLOGY;

EID: 82055190171     PISSN: 20366590     EISSN: 19722680     Source Type: Journal    
DOI: 10.3855/jidc.1538     Document Type: Article

References (27)

1. Corbel MJ (1997) Brucellosis: an overview. Emerg Infect Dis 3: 213-221.
2. Boschiroli ML, Foulongne V, O'Callaghan D (2001) Brucellosis: a worldwide zoonosis. Curr Opin Microbiol 4: 58-64.
3. Pappas G and Memish ZA (2007) Brucellosis in the Middle East: a persistent medical, socioeconomic and political issue. J Chemother 19: 243-248.
4. Afifi S, Earhart K, Azab MA, Youssef FG, El Sakka H, Wasfy M, Mansour H, El Oun S, Rakha M, Mahoney F (2005) Hospital-based surveillance for acute febrile illness in Egypt: a focus on community-acquired bloodstream infections. Am J Trop Med Hyg 73: 392-399.
5. Samaha H, Mohamed TR, Khoudair RM, Ashour HM (2009) Serodiagnosis of brucellosis in cattle and humans in Egypt. Immunobiology 214: 223-226.
6. Araj GF (1999) Human brucellosis: a classical infectious disease with persistent diagnostic challenges. Clin Lab Sci 12: 207-212.
7. Al Dahouk S, Tomaso H, Nockler K, Neubauer H, Frangoulidis D (2003) Laboratory-based diagnosis of brucellosis--a review of the literature. Part II: serological tests for brucellosis. Clin Lab 49: 577-589.
8. Espinosa BJ, Chacaltana J, Mulder M, Franco MP, Blazes DL, Gilman RH, Smits HL, Hall ER (2009) Comparison of culture techniques at different stages of brucellosis. Am J Trop Med Hyg 80: 625-627.
9. Young EJ (1995) An overview of human brucellosis. Clin Infect Dis 21: 283-289; quiz 290.
10. Franco MP, Mulder M, Gilman RH, Smits HL (2007) Human brucellosis. Lancet Infect Dis 7: 775-786.
11. Ariza J, Bosilkovski M, Cascio A, Colmenero JD, Corbel MJ, Falagas ME, Memish ZA, Roushan MR, Rubinstein E, Sipsas NV, Solera J, Young EJ, Pappas G (2007) Perspectives for the treatment of brucellosis in the 21st century: the Ioannina recommendations. PLoS Med 4, e317.
12. Maas KS, Mendez M, Zavaleta M, Manrique J, Franco MP, Mulder M, Bonifacio N, Castaneda ML, Chacaltana J, Yagui E, Gilman RH, Guillen A, Blazes DL, Espinosa B, Hall E, Abdoel TH, Smits HL (2007) Evaluation of brucellosis by PCR and persistence after treatment in patients returning to the hospital for follow-up. Am J Trop Med Hyg 76: 698-702.
13. Mitka S, Anetakis C, Souliou E, Diza E, Kansouzidou A (2007) Evaluation of different PCR assays for early detection of acute and relapsing brucellosis in humans in comparison with conventional methods. J Clin Microbiol 45: 1211-1218.
14. Navarro E, Segura JC, Castano MJ, Solera J (2006) Use of real-time quantitative polymerase chain reaction to monitor the evolution of Brucella melitensis DNA load during therapy and post-therapy follow-up in patients with brucellosis. Clin Infect Dis 42: 1266-1273.
15. Keramat F, Ranjbar M, Mamani M, Hashemi SH, Zeraati F (2009) A comparative trial of three therapeutic regimens: ciprofloxacin-rifampin, ciprofloxacin-doxycycline and doxycycline-rifampin in the treatment of brucellosis. Trop Doct 39: 207-210.
16. Mantur BG and Mangalgi SS (2004) Evaluation of conventional Castaneda and lysis centrifugation blood culture techniques for diagnosis of human Brucellosis. J Clin Microbiol 42: 4327-4328.
17. Morgan WJ, Mackinnon DJ, Lawson JR, Cullen GA (1969) The rose bengal plate agglutination test in the diagnosis of brucellosis. Vet Rec 85: 636-641.
18. Klein GC and Behan KA (1981) Determination of brucella immunoglobulin G agglutinating antibody titer with dithiothreitol. J Clin Microbiol 14: 24-25.
19. Smits HL, Abdoel TH, Solera J, Clavijo E, Diaz R (2003) Immunochromatographic Brucella-specific immunoglobulin M and G lateral flow assays for rapid serodiagnosis of human brucellosis. Clin Diagn Lab Immunol 10: 1141-1146.
20. Baily GG, Krahn JB, Drasar BS, Stoker NG (1992) Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 95: 271-275.
21. Irmak H, Buzgan T, Evirgen O, Akdeniz H, Demiroz AP, Abdoel TH, Smits HL (2004) Use of the Brucella IgM and IgG flow assays in the serodiagnosis of human brucellosis in an area endemic for brucellosis. Am J Trop Med Hyg 70: 688-694.
22. Zeytinoglu A, Turhan A, Altuglu I, Bilgic A, Abdoel TH, Smits HL (2006) Comparison of Brucella immunoglobulin M and G flow assays with serum agglutination and 2-mercaptoethanol tests in the diagnosis of brucellosis. Clin Chem Lab Med 44: 180-184.
23. Mizanbayeva S, Smits HL, Zhalilova K, Abdoel TH, Kozakov S, Ospanov KS, Elzer PH, Douglas JT (2009) The evaluation of a user-friendly lateral flow assay for the serodiagnosis of human brucellosis in Kazakhstan. Diagn Microbiol Infect Dis 65: 14-20.
24. Cooper CW (1992) Prevalence of antibody to Brucella in asymptomatic well individuals in Saudi Arabia. J Trop Med Hyg 95: 140-142.
25. Morata P, Queipo-Ortuno MI, Reguera JM, Garcia-Ordonez MA, Pichardo C, Colmenero JD (1999) Posttreatment follow-Up of brucellosis by PCR assay. J Clin Microbiol 37: 4163-4166.
26. Bricker BJ (2002) PCR as a diagnostic tool for brucellosis. Vet Microbiol 90: 435-446.
27. Navarro E, Casao MA, Solera J (2004) Diagnosis of human brucellosis using PCR. Expert Rev Mol Diagn 4: 115-123.