The polypyrimidine tract binding protein regulates desaturase alternative splicing and PUFA composition

Journal of Lipid Research

Volumn 52, Issue 12, 2011, Pages 2279-2286

  Reardon, Holly T ^a   Park, Woo Jung ^a   Zhang, Jimmy ^a   Lawrence, Peter ^a   Kothapalli, Kumar S D ^a   Brenna, J Thomas ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

6 desaturase; Alternative splicing; FADS2; Inflammation; N 3 fatty acids; Polyunsaturated fatty acids; PTB

Indexed keywords

ACYL COENZYME A DESATURASE; ARACHIDONIC ACID; ICOSANOID; ICOSAPENTAENOIC ACID; MESSENGER RNA PRECURSOR; OMEGA 3 FATTY ACID; OMEGA 6 FATTY ACID; POLYPYRIMIDINE TRACT BINDING PROTEIN; POLYUNSATURATED FATTY ACID; RNA;

ALTERNATIVE RNA SPLICING; ANIMAL TISSUE; ARTICLE; BABOON; CELL STRAIN HEPG2; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME REGULATION; EXON; FATTY ACID SYNTHESIS; HUMAN; HUMAN CELL; LIPID COMPOSITION; NEWBORN; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; SIGNAL TRANSDUCTION; SILENCER ELEMENT; TISSUE SPECIFICITY;

ALTERNATIVE SPLICING; ANIMALS; EXONS; FATTY ACID DESATURASES; FATTY ACIDS, OMEGA-3; FATTY ACIDS, OMEGA-6; GENE KNOCKDOWN TECHNIQUES; GENE SILENCING; HEP G2 CELLS; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEINS; HUMANS; PAPIO; POLYPYRIMIDINE TRACT-BINDING PROTEIN; PROTEIN BINDING; RNA SPLICE SITES; RNA, SMALL INTERFERING; UP-REGULATION;

EID: 81855206691     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.M019653     Document Type: Article

References (45)

1. Brenna, J. T., and G. Y. Diau. 2007. The influence of dietary docosahexaenoic acid and arachidonic acid on central nervous system polyunsaturated fatty acid composition. Prostaglandins Leukot. Essent. Fatty Acids. 77: 247-250. (Pubitemid 350198855)
2. Dobbing, J., and J. Sands. 1979. Comparative aspects of the brain growth spurt. Early Hum. Dev. 3: 79-83. (Pubitemid 9141710)
3. Martinez, M. 1992. Tissue levels of polyunsaturated fatty acids during early human development. J. Pediatr. 120: S129-S138.
4. Calder, P. C. 2009. Polyunsaturated fatty acids and inflammatory processes: new twists in an old tale. Biochimie. 91: 791-795.
5. Schmitz, G., and J. Ecker. 2008. The opposing effects of n-3 and n-6 fatty acids. Prog. Lipid Res. 47: 147-155.
6. Aulchenko, Y. S., S. Ripatti, I. Lindqvist, D. Boomsma, I. M. Heid, P. P. Pramstaller, B. W. Penninx, A. C. Janssens, J. F. Wilson, T. Spector, et al. 2009. Loci influencing lipid levels and coronary heart disease risk in 16 European population cohorts. Nat. Genet. 41: 47-55.
7. Martinelli, N., D. Girelli, G. Malerba, P. Guarini, T. Illig, E. Trabetti, M. Sandri, S. Friso, F. Pizzolo, L. Schaeffer, et al. 2008. FADS genotypes and desaturase activity estimated by the ratio of arachidonic acid to linoleic acid are associated with inflammation and coronary artery disease. Am. J. Clin. Nutr. 88: 941-949.
8. Brookes, K. J., W. Chen, X. Xu, E. Taylor, and P. Asherson. 2006. Association of fatty acid desaturase genes with attention-deficit/hyperactivity disorder. Biol. Psychiatry. 60: 1053-1061. (Pubitemid 44647221)
9. Caspi, A., B. Williams, J. Kim-Cohen, I. W. Craig, B. J. Milne, R. Poulton, L. C. Schalkwyk, A. Taylor, H. Werts, and T. E. Moffitt. 2007. Moderation of breastfeeding effects on the IQ by genetic variation in fatty acid metabolism. Proc. Natl. Acad. Sci. USA. 104: 18860-18865. (Pubitemid 350210787)
10. Steer, C. D., G. Davey Smith, P. M. Emmett, J. R. Hibbeln, and J. Golding. 2010. FADS2 polymorphisms modify the effect of breastfeeding on child IQ. PLoS ONE. 5: e11570.
11. Schaeffer, L., H. Gohlke, M. Muller, I. M. Heid, L. J. Palmer, I. Kompauer, H. Demmelmair, T. Illig, B. Koletzko, and J. Heinrich. 2006. Common genetic variants of the FADS1 FADS2 gene cluster and their reconstructed haplotypes are associated with the fatty acid composition in phospholipids. Hum. Mol. Genet. 15: 1745-1756. (Pubitemid 43821765)
12. Park, W. J., H. T. Reardon, C. Tyburczy, K. S. Kothapalli, and J. T. Brenna. 2010. Alternative splicing generates a novel FADS2 alternative transcript in baboons. Mol. Biol. Rep. 37: 2403-2406.
13. Brenna, J. T., K. S. Kothapalli, and W. J. Park. 2010. Alternative transcripts of fatty acid desaturase (FADS) genes. Prostaglandins Leukot. Essent. Fatty Acids. 82: 281-285.
14. Park, W. J., K. S. Kothapalli, H. T. Reardon, L. Y. Kim, and J. T. Brenna. 2009. Novel fatty acid desaturase 3 (FADS3) transcripts generated by alternative splicing. Gene. 446: 28-34.
15. Plaisier, C. L., S. Horvath, A. Huertas-Vazquez, I. Cruz-Bautista, M. F. Herrera, T. Tusie-Luna, C. Aguilar-Salinas, and P. Pajukanta. 2009. A systems genetics approach implicates USF1, FADS3, and other causal candidate genes for familial combined hyperlipidemia. PLoS Genet. 5: e1000642.
16. Hicks, A. A., P. P. Pramstaller, A. Johansson, V. Vitart, I. Rudan, P. Ugocsai, Y. Aulchenko, C. S. Franklin, G. Liebisch, J. Erdmann, et al. 2009. Genetic determinants of circulating sphingolipid concentrations in European populations. PLoS Genet. 5: e1000672.
17. Ma, X. H., S. J. Hu, H. Ni, Y. C. Zhao, Z. Tian, J. L. Liu, G. Ren, X. H. Liang, H. Yu, P. Wan, et al. 2006. Serial analysis of gene expression in mouse uterus at the implantation site. J. Biol. Chem. 281: 9351-9360. (Pubitemid 43864652)
18. House, A. E., and K. W. Lynch. 2008. Regulation of alternative splicing: more than just the ABCs. J. Biol. Chem. 283: 1217-1221.
19. Paradis, C., P. Cloutier, L. Shkreta, J. Toutant, K. Klarskov, and B. Chabot. 2007. hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA. 13: 1287-1300. (Pubitemid 47080474)
20. Sawicka, K., M. Bushell, K. A. Spriggs, and A. E. Willis. 2008. Polypyrimidine-tract-binding protein: a multifunctional RNAbinding protein. Biochem. Soc. Trans. 36: 641-647.
21. He, X., M. Pool, K. M. Darcy, S. B. Lim, N. Auersperg, J. S. Coon, and W. T. Beck. 2007. Knockdown of polypyrimidine tract-binding protein suppresses ovarian tumor cell growth and invasiveness in vitro. Oncogene. 26: 4961-4968. (Pubitemid 47172653)
22. Rustighi, A., M. A. Tessari, F. Vascotto, R. Sgarra, V. Giancotti, and G. Manfioletti. 2002. A polypyrimidine/polypurine tract within the Hmga2 minimal promoter: a common feature of many growthrelated genes. Biochemistry. 41: 1229-1240. (Pubitemid 34083775)
23. Knoch, K. P., H. Bergert, B. Borgonovo, H. D. Saeger, A. Altkruger, P. Verkade, and M. Solimena. 2004. Polypyrimidine tract-binding protein promotes insulin secretory granule biogenesis. Nat. Cell Biol. 6: 207-214. (Pubitemid 38344358)
24. Stamm, S., J. J. Riethoven, V. Le Texier, C. Gopalakrishnan, V. Kumanduri, Y. Tang, N. L. Barbosa-Morais, and T. A. Thanaraj. 2006. ASD: a bioinformatics resource on alternative splicing. Nucleic Acids Res. 34: D46-D55.
25. Jackson, A. L., J. Burchard, D. Leake, A. Reynolds, J. Schelter, J. Guo, J. M. Johnson, L. Lim, J. Karpilow, K. Nichols, et al. 2006. Position-specific chemical modification of siRNAs reduces "off-target"transcript silencing. RNA. 12: 1197-1205. (Pubitemid 43990545)
26. Hsieh, A. T., J. C. Anthony, D. A. Diersen-Schade, S. C. Rumsey, P. Lawrence, C. Li, P. W. Nathanielsz, and J. T. Brenna. 2007. The infl uence of moderate and high dietary long chain polyunsaturated fatty acids (LCPUFA) on baboon neonate tissue fatty acids. Pediatr. Res. 61: 537-545. (Pubitemid 46650004)
27. Pfaffl, M. W. 2001. A new mathematical model for relative quantifi- cation in real-time RT-PCR. Nucleic Acids Res. 29: e45.
28. Garcés, R., and M. Mancha. 1993. One-step lipid extraction and fatty acid methyl esters preparation from fresh plant tissues. Anal. Biochem. 211: 139-143. (Pubitemid 23189341)
29. Diau, G. Y., A. T. Hsieh, E. A. Sarkadi-Nagy, V. Wijendran, P. W. Nathanielsz, and J. T. Brenna. 2005. The influence of long chain polyunsaturate supplementation on docosahexaenoic acid and arachidonic acid in baboon neonate central nervous system. BMC Med. 3: 11.
30. Van Pelt, C. K., and J. T. Brenna. 1999. Acetonitrile chemical ionization tandem mass spectrometry to locate double bonds in polyunsaturated fatty acid methyl esters. Anal. Chem. 71: 1981-1989. (Pubitemid 29233161)
31. Lawrence, P., and J. T. Brenna. 2006. Acetonitrile covalent adduct chemical ionization mass spectrometry for double bond localization in non-methylene-interrupted polyene fatty acid methyl esters. Anal. Chem. 78: 1312-1317. (Pubitemid 43271866)
32. Michaud, A. L., G. Y. Diau, R. Abril, and J. T. Brenna. 2002. Double bond localization in minor homoallylic fatty acid methyl esters using acetonitrile chemical ionization tandem mass spectrometry. Anal. Biochem. 307: 348-360. (Pubitemid 35026211)
33. Spellman, R., M. Llorian, and C. W. Smith. 2007. Crossregulation and functional redundancy between the splicing regulator PTB and its paralogs nPTB and ROD1. Mol. Cell. 27: 420-434. (Pubitemid 47126939)
34. Morais, S., J. Pratoomyot, J. B. Taggart, J. E. Bron, D. R. Guy, J. G. Bell, and D. R. Tocher. 2011. Genotype-specific responses in Atlantic salmon (Salmo salar) subject to dietary fish oil replacement by vegetable oil: a liver transcriptomic analysis. BMC Genomics. 12: 255.
35. Kolditz, C. I., G. Paboeuf, M. Borthaire, D. Esquerre, M. SanCristobal, F. Lefevre, and F. Medale. 2008. Changes induced by dietary energy intake and divergent selection for muscle fat content in rainbow trout (Oncorhynchus mykiss), assessed by transcriptome and proteome analysis of the liver. BMC Genomics. 9: 506.
36. Jordal, A. E., B. E. Torstensen, S. Tsoi, D. R. Tocher, S. P. Lall, and S. E. Douglas. 2005. Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.). J. Nutr. 135: 2355-2361. (Pubitemid 41429590)
37. Panserat, S., G. Hortopan, E. Plagnes-Juan, C. Kolditz, M. Lansard, S. Skiba-Cassy, D. Esquerre, I. Guerden, F. Medale, S. Kaushik, et al. 2009. Differential gene expression after total replacement of dietary fish meal and fish oil by plant products in rainbow trout (Oncorhynchus mykiss) liver. Aquaculture. 294: 123-131.
38. Marzo, I., M. A. Alava, A. Pineiro, and J. Naval. 1996. Biosynthesis of docosahexaenoic acid in human cells: evidence that two different delta 6-desaturase activities may exist. Biochim. Biophys. Acta. 1301: 263-272. (Pubitemid 26195797)
39. Inagaki, K., T. Aki, T. Shiota, S. Kawamoto, S. Shigeta, O. Suzuki, and K. Ono. 2003. Evidence of isozymes for delta6 fatty acid desaturase in rat hepatocytes. Biosci. Biotechnol. Biochem. 67: 451-454. (Pubitemid 39252013)
40. Cho, H. P., M. T. Nakamura, and S. D. Clarke. 1999. Cloning, expression, and nutritional regulation of the mammalian Delta-6 desaturase. J. Biol. Chem. 274: 471-477. (Pubitemid 29035085)
41. Stamm, S., S. Ben-Ari, I. Rafalska, Y. Tang, Z. Zhang, D. Toiber, T. A. Thanaraj, and H. Soreq. 2005. Function of alternative splicing. Gene. 344: 1-20. (Pubitemid 40126788)
42. Niu, S. L., D. C. Mitchell, S. Y. Lim, Z. M. Wen, H. Y. Kim, N. Salem, Jr., and B. J. Litman. 2004. Reduced G protein-coupled signaling efficiency in retinal rod outer segments in response to n-3 fatty acid deficiency. J. Biol. Chem. 279: 31098-31104. (Pubitemid 39037771)
43. Hsieh, A. T., and J. T. Brenna. 2009. Dietary docosahexaenoic acid but not arachidonic acid influences central nervous system fatty acid status in baboon neonates. Prostaglandins Leukot. Essent. Fatty Acids. 81: 105-110.
44. Makheja, A. N. 1992. Atherosclerosis: the eicosanoid connection. Mol. Cell. Biochem. 111: 137-142.
45. D'Andrea, S., H. Guillou, S. Jan, D. Catheline, J. N. Thibault, M. Bouriel, V. Rioux, and P. Legrand. 2002. The same rat Delta6- desaturase not only acts on 18- but also on 24-carbon fatty acids in very-long-chain polyunsaturated fatty acid biosynthesis. Biochem. J. 364: 49-55. (Pubitemid 34538945)