Affinity transfer to a human protein by CDR3 grafting of camelid VHH

Protein Science

Volumn 20, Issue 12, 2011, Pages 1971-1981

  Inoue, Hidetoshi ^a,b   IIhara, Akiko ^b,c   Takahashi, Hideo ^c,d   Shimada, Ichio ^c,e   Ishida, Isao ^a   Maeda, Yoshitake ^a,b  

^a KYOWA HAKKO KOGYO CO LTD   (Japan)

^b Japan Biological Informatics Consortium   (Japan)

^c NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^d YOKOHAMA CITY UNIVERSITY   (Japan)

^e UNIVERSITY OF TOKYO   (Japan)

Author keywords

Affinity transfer; CDR grafting; NMR analysis; Protein stability; Scaffold; Thermodynamic parameter; VHH

Indexed keywords

ANTIGEN; CYSTEINE; PROTEIN VHH; SCAFFOLD PROTEIN; UBIQUITIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANTIGEN BINDING; ARTICLE; ARTIODACTYLA; CARBOXY TERMINAL SEQUENCE; DISULFIDE BOND; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STABILITY; PROTEIN STRUCTURE; THERMODYNAMICS;

AMINO ACID SEQUENCE; ANIMALS; CAMELIDS, NEW WORLD; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN HEAVY CHAINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; UBIQUITIN;

CAMELIDAE;

EID: 81755162788     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.734     Document Type: Article

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