High-resolution detection of 13C multiplets from the conscious mouse brain by ex vivo NMR spectroscopy

Journal of Neuroscience Methods

Volumn 203, Issue 1, 2012, Pages 50-55

  Marin Valencia, Isaac ^a   Good, Levi B ^a   Ma, Qian ^a   Jeffrey, F Mark ^a,b   Malloy, Craig R ^a,b   Pascual, Juan M ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

13C; Brain metabolism; Glucose; Glutamate; Glutamine; Mouse; Multiplets; NMR spectroscopy; Stable isotopes

Indexed keywords

4 AMINOBUTYRIC ACID; ACETYL COENZYME A; ASPARTIC ACID; CARBON 13; CITRIC ACID; GLUCOSE; GLUTAMIC ACID; GLUTAMINE; LACTIC ACID; N ACETYLASPARTIC ACID; OXALOACETIC ACID; TAURINE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; BRAIN METABOLISM; CARBON NUCLEAR MAGNETIC RESONANCE; CITRIC ACID CYCLE; CONSCIOUSNESS; CONTROLLED STUDY; EX VIVO STUDY; FEMALE; GLUCOSE INFUSION; GLUCOSE METABOLISM; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; SIGNAL NOISE RATIO; STEADY STATE; WAKEFULNESS;

ANIMALS; BRAIN; CARBON RADIOISOTOPES; CONSCIOUSNESS; FEMALE; GLUCOSE; MAGNETIC RESONANCE SPECTROSCOPY; MICE; MICE, INBRED C57BL;

EID: 81555214148     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2011.09.006     Document Type: Article

References (29)

1. 13C] NMR spectroscopy. Neurochem Int 2005, 47:143-151.
2. Ardenkjaer-Larsen J.H., Fridlund B., Gram A., Hansson G., Hansson L., Lerche M.H., et al. Increase in signal-to-noise ratio of >10,000 times in liquid-state NMR. Proc Natl Acad Sci USA 2003, 100:10158-10163.
3. Ayala J.E., Bracy D.P., McGuinness O.P., Wasserman D.H. Considerations in the design of hyperinsulinemic-euglycemic clamps in the conscious mouse. Diabetes 2006, 55:390-397.
4. Chang J.Y., Martin P., Bernasconi R., Braun D.G. High-sensitivity amino acid analysis: measurement of amino acid neurotransmitter in mouse brain. FEBS Lett 1981, 132:117-120.
5. 13C]glucose in the rat brain in vivo. J Neurochem 2004, 91:778-787.
6. Choi I.Y., Lei H., Gruetter R. Effect of deep pentobarbital anesthesia on neurotransmitter metabolism in vivo: on the correlation of total glucose consumption with glutamatergic action. J Cereb Blood Flow Metab 2002, 22:1343-1351.
7. Dobbins R.L., Malloy C.R. Measuring in-vivo metabolism using nuclear magnetic resonance. Curr Opin Clin Nutr Metab Care 2003, 6:501-509.
8. Escartin C., Valette J., Lebon V., Bonvento G. Neuron-astrocyte interactions in the regulation of brain energy metabolism: a focus on NMR spectroscopy. J Neurochem 2006, 99:393-401.
9. Hassel B., Bachelard H., Jones P., Fonnum F., Sonnewald U. Trafficking of amino acids between neurons and glia in vivo. Effects of inhibition of glial metabolism by fluoroacetate. J Cereb Blood Flow Metab 1997, 17:1230-1238.
10. Hassel B., Westergaard N., Schousboe A., Fonnum F. Metabolic differences between primary cultures of astrocytes and neurons from cerebellum and cerebral cortex. Effects of fluorocitrate. Neurochem Res 1995, 20:413-420.
11. Jeffrey F.M., Reshetov A., Storey C.J., Carvalho R.A., Sherry A.D., Malloy C.R. Use of a single (13)C NMR resonance of glutamate for measuring oxygen consumption in tissue. Am J Physiol 1999, 277:E1111-E1121.
12. Kovacs H., Moskau D., Spraul M. Cryogenically cooled probes-a leap in NMR technology. Prog Nucl Magn Reson Spectrosc 2005, 46:131-155.
13. 13C NMR spectroscopy. FEBS Lett 1987, 212:58-62.
14. 13C NMR under non-steady-state conditions. Biochemistry 1990, 29:6756-6761.
15. 13C MRS to detect glucose metabolism in the mouse brain. Magn Reson Med 2008, 59:626-630.
16. 2 fixation increases with brain activity. J Neurosci 2004, 24:11273-11279.
17. Patel A.B., de Graaf R.A., Mason G.F., Kanamatsu T., Rothman D.L., Shulman R.G., et al. Glutamatergic neurotransmission and neuronal glucose oxidation are coupled during intense neuronal activation. J Cereb Blood Flow Metab 2004, 24:972-985.
18. Peled-Kamar M., Degani H., Bendel P., Margalit R., Groner Y. Altered brain glucose metabolism in transgenic-PFKL mice with elevated l-phosphofructokinase: in vivo NMR studies. Brain Res 1998, 810:138-145.
19. 13C NMR. Magn Reson Med 1993, 30:262-266.
20. Schurr A. Neuronal energy requirements. The neuronal environment: brain homeostasis in health and disease 2002, 25-54. Humana Press, New Jersey. W. Walz (Ed.).
21. 13C isotopomer analysis of glutamate. A NMR method to probe metabolic pathways intersecting in the citric acid cycle. Biological magnetic resonance. In vivo carbon-13 NMR 1998, 59-97. Kluwer Academic /Plenum Publishers, New York. P.-M. Robitaille, L.J. Berliner (Eds.).
22. Sibson N.R., Dhankhar A., Mason G.F., Rothman D.L., Behar K.L., Shulman R.G. Stoichiometric coupling of brain glucose metabolism and glutamatergic neuronal activity. Proc Natl Acad Sci USA 1998, 95:316-321.
23. Sibson N.R., Mason G.F., Shen J., Cline G.W., Herskovits A.Z., Wall J.E., et al. In vivo (13)C NMR measurement of neurotransmitter glutamate cycling, anaplerosis and TCA cycle flux in rat brain during. J Neurochem 2001, 76:975-989.
24. Sonnewald U., Muller T.B., Westergaard N., Unsgard G., Petersen S.B., Schousboe A. NMR spectroscopic study of cell cultures of astrocytes and neurons exposed to hypoxia: compartmentation of astrocyte metabolism. Neurochem Int 1994, 24:473-483.
25. Tansey F.A., Farooq M., Cammer W. Glutamine synthetase in oligodendrocytes and astrocytes: new biochemical and immunocytochemical evidence. J Neurochem 1991, 56:266-272.
26. Walls A.B., Eyjolfsson E.M., Smeland O.B., Nilsen L.H., Schousboe I., Schousboe A., et al. Knockout of GAD65 has major impact on synaptic GABA synthesized from astrocyte-derived glutamine. J Cereb Blood Flow Metab 2011, 31:494-503.
27. 13C NMR spectroscopy. J Physiol 2003, 548:541-548.
28. 13C-glutamate labelling by the tricarboxylic acid cycle in intact rat hearts. Biochim Biophys Acta 1995, 1243:543-548.
29. 13C magnetization transfer spectroscopy. J Cereb Blood Flow Metab 2009, 29:661-669.