Biofilm extracellular-DNA in 55 Staphylococcus epidermidis clinical isolates from implant infections

International Journal of Artificial Organs

Volumn 34, Issue 9, 2011, Pages 840-846

  Ravaioli, Stefano ^a,b   Campoccia, Davide ^a   Visai, Livia ^c,d   Pirini, Valter ^a   Cangini, Ilaria ^a,b   Corazzari, Tolmino ^e   Maso, Alessandra ^a   Poggio, Claudio ^c   Pegreffi, Francesco ^c   Montanaro, Lucio ^a,b   Arciola, Carla Renata ^a,b  

^a RIZZOLI ORTHOPAEDIC INSTITUTE   (Italy)

^b UNIVERSITY OF BOLOGNA   (Italy)

^c UNIVERSITY OF PAVIA   (Italy)

^d International Center For Studies and Research In Biomedicine (ICB)   (Italy)

^e UNIVERSITY OF MODENA AND REGGIO EMILIA   (Italy)

Author keywords

Biofilm; Exopopolysaccaride; Extracellular DNA; Implant orthopedic infections; Staphylococcus epidermidis

Indexed keywords

BACTERIA; BIOFILMS; NONDESTRUCTIVE EXAMINATION;

BACTERIAL BIOFILM; BIOFILM FORMATION; CLINICAL ISOLATES; EPIDERMIDIS; EXOPOPOLYSACCARIDE; EXTRACELLULAR; EXTRACELLULAR DNA; IMPLANT INFECTIONS; IMPLANT ORTHOPEDIC INFECTION; STAPHYLOCOCCUS EPIDERMIDIS;

DNA;

BIOMATERIAL; DNA; EXTRACELLULAR DNA; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM ISOLATE; BIOFILM; FLUOROMETRY; HUMAN; MICROTITER PLATE ASSAY; NONHUMAN; PROSTHESIS INFECTION; STAPHYLOCOCCUS EPIDERMIDIS;

EID: 81355163352     PISSN: 03913988     EISSN: None     Source Type: Journal    
DOI: 10.5301/ijao.5000057     Document Type: Article

References (30)

1. Arciola CR, Campoccia D, Gamberini S, et al. Antibiotic resistance in exopolysaccharide-forming Staphylococcus epidermidis clinical isolates from orthopaedic implant infections. Biomaterials. 2005;26(33):6530-6535.
2. Arciola CR, An YH, Campoccia D, Donati ME, Montanaro L. Etiology of implant orthopedic infections: a survey on 1027 clinical isolates. Int J Artif Organs. 2005;28(11):1091-1100.
3. Costerton JW, Montanaro L, Arciola CR. Biofilm in implant infections: its production and regulation. Int J Artif Organs. 2005;28(11):1062-1068.
4. Heilmann C, Schweitzer O, Gerke C, Vanittanakom N, Mack D, Götz F. Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis. Mol Microbiol. 1996;20(5):1083-1091. doi:10.1111/j.1365-2958.1996.tb02548.x.
5. Bowden MG, Chen W, Singvall J, et al. Identification and preliminary characterization of cell-wall-anchored proteins of Staphylococcus epidermidis. Microbiology. 2005;151(5):1453-1464. doi:10.1099/mic.0.27534-0.
6. Fey PD, Olson ME. Current concepts in biofilm formation of Staphylococcus epidermidis. Future Microbiol. 2010;5(6):917-933. doi:10.2217/fmb.10.56.
7. Vuong C, Otto M. The biofilm exopolysaccharide polysaccharide intercellular adhesion-a molecular and biochemical approach. Methods Mol Biol. 2008;431:97-105. doi:10.1007/978-1-60327-032-8_8.
8. Izano EA, Amarante MA, Kher WB, Kaplan JB. Differential roles of poly-N-acetylglucosamine surface polysaccharide and extracellular DNA in Staphylococcus aureus and Staphylococcus epidermidis biofilms. Appl Environ Microbiol. 2008;74(2):470-476. doi:10.1128/AEM.02073-07.
9. Wingender J, Neu T, Flemming, H-C, eds. Microbial Extracellular Polymeric Substances. Heidelberg: Springer;1999:1-19.
10. Frølund B, Palmgren R, Keiding K, Nielsen P-H. Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Res. 1996;30(8):1749-1758. doi:10.1016/0043-1354(95)00323-1.
11. Thomas VC, Hancock LE. Suicide and fratricide in bacterial biofilms. Int J Artif Organs. 2009;32(9):537-544. Review.
12. Whitchurch CB, Tolker-Nielsen T, Ragas PC, Mattick JS. Extracellular DNA required for bacterial biofilm formation. Science. 2002; 295(5559): 1487.
13. Mulcahy H, Charron-Mazenod L, Lewenza S. Extracellular DNA chelates and induces antibiotic resistance in Pseudomonas aeruginosa biofilms. PLoS Pathog. 2008;4(11):e1000213. doi:10.1371/journal.ppat.1000213.
14. Fey PD. Modality of bacterial growth presents unique targets: how do we treat biofilm-mediated infections? Curr Opin Microbiol. 2010;13(5):610-615. doi:10.1016/j.mib.2010.09.007.
15. Kaplan JB, Jabbouri S, Sadovskaya I. Extracellular DNAdependent biofilm formation by Staphylococcus epidermidis RP62A in response to subminimal inhibitory concentrations of antibiotics. Res Microbiol. 2011;162(5):535-541. doi:10.1016/j.resmic.2011.03.008.
16. Campoccia D, Montanaro L, von Eiff C, et al. Cluster analysis of ribotyping profiles of Staphylococcus epidermidis isolates recovered from foreign body-associated orthopedic infections. J Biomed Mater Res A. 2009;88A(3):664-672. doi:10.1002/jbm.a.32090.
17. Campoccia D, Montanaro L, Visai L, et al. Characterization of 26 Staphylococcus warneri isolates from orthopedic infections. Int J Artif Organs 2010; 33(9): 575-581.
18. Arciola CR, Baldassarri L, Montanaro L. In catheter in fections by Staphylococcus epidermidis the intercellular adhesion (ica) locus is a molecular marker of the virulent slime-producing strains. J Biomed Mater Res. 2002; 59(3): 557-562.
19. Rohde H, Kalitzky M, Kröger N, et al. Detection of virulenceassociated genes not useful for discriminating between invasive and commensal Staphylococcus epidermidis strains from a bone marrow transplant unit. J Clin Microbiol. 2004;42(12):5614-5619. doi:10.1128/JCM.42.12.5614-5619.2004.
20. Campoccia D, Montanaro L, Ravaioli S, Pirini V, Cangini I, Arciola CR. Exopolysaccharide production by Staphylococcus epidermidis and its relationship with biofilm extracellular- DNA. Int J Artif Organs. 2011;34: (9):832-839.
21. Qin Z, Ou Y, Yang L, et al. Role of autolysin-mediated DNA release in biofilm formation of Staphylococcus epidermidis. Microbiology. 2007;153(7):2083-2092. doi:10.1099/ mic.0.2007/006031-0.
22. Lou Q, Zhu T, Hu J, et al. Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation. BMC Microbiol. 2011;11(1):146. doi:10.1186/1471-2180-11-146.
23. Arciola CR, Baldassarri L, Campoccia D, et al. Strong biofilm production, antibiotic multi-resistance and high gelE expression in epidemic clones of Enterococcus faecalis from orthopaedic implant infections. Biomaterials. 2008;29(5):580-586. doi:10.1016/j.biomaterials. 2007.10.008.
24. Costerton WJ, Montanaro L, Balaban N, Arciola CR. Prospecting gene therapy of implant infections. Int J Artif Organs. 2009;32(9):689-695. Review.
25. Groll J, Fiedler J, Bruellhoff K, Moeller M, Brenner RE. Novel surface coatings modulating eukaryotic cell adhesion and preventing implant infection. Int J Artif Organs. 2009;32(9):655-662. Review.
26. Bruellhoff K, Fiedler J, Möller M, Groll J, Brenner RE. Surface coating strategies to prevent biofilm formation on implant surfaces. Int J Artif Organs. 2010;33(9):646-653. Review.
27. Arciola CR. New concepts and new weapons in implant infections. Int J Artif Organs. 2009;32(9):533-536.
28. JB. Therapeutic potential of biofilm-dispersing enzymes. Int J Artif Organs. 2009;32(9):545-554. Review
29. Jäger S, Jonas B, Pfanzelt D, et al. Regulation of biofilm formation by sigma B is a common mechanism in Staphylococcus epidermidis and is not mediated by transcriptional regulation of sarA. Int J Artif Organs. 2009;32(9):584-591.
30. Høiby N, Ciofu O, Johansen HK, et al. The clinical impact of bacterial biofilms. Int J Oral Sci 2011;3(2):55-65. doi:10.4248/IJOS11026.