Purification and characterization of a novel aspartic protease from basidiomycetous yeast Cryptococcus sp. S-2

Journal of Bioscience and Bioengineering

Volumn 112, Issue 5, 2011, Pages 441-446

  Rao, Shengbin ^a,b   Mizutani, Osamu ^b   Hirano, Takuya ^a,b   Masaki, Kazuo ^b   Iefuji, Haruyuki ^a,b  

^a HIROSHIMA UNIVERSITY   (Japan)

^b NATIONAL RESEARCH INSTITUTE OF BREWING   (Japan)

Author keywords

Basidiomycetous; Cloning; Cryptococcus; Extracellular; Protease; Purification

Indexed keywords

AMINO ACID RESIDUES; AMINO ACID SEQUENCE; ASPARTIC PROTEASE; AZOCASEIN; BASIDIOMYCETOUS; CLEAVAGE PRODUCTS; CRYPTOCOCCUS; CRYPTOCOCCUS SP; EXTRACELLULAR; HOMOLOGY SEARCH; HUMAN PEPSIN; MILK-CLOTTING ACTIVITY; OPEN READING FRAME; PEPSIN A; PEPTIDE BONDS; PH RANGE; PROTEASE; PROTEIN SUBSTRATE; PROTEOLYTIC ACTIVITIES; SDS-PAGE; SEQUENCING ANALYSIS; SYNTHETIC SUBSTRATES;

AMINO ACIDS; CASEIN; CLONING; COLUMN CHROMATOGRAPHY; GENE ENCODING; HYDROLYSIS; PROTEINS; PROTEOLYSIS; PURIFICATION; SUBSTRATES; YEAST;

ENZYME ACTIVITY;

ALPHA CASEIN; AMINO ACID; ASPARTIC PROTEINASE; BETA CASEIN; COMPLEMENTARY DNA; HEMOGLOBIN; INSULIN; KAPPA CASEIN; PEPSIN A; PEPSTATIN; PEPTIDE DERIVATIVE;

ACIDITY; AMINO ACID SEQUENCE; ARTICLE; BASIDIOMYCETES; BETA CHAIN; COLUMN CHROMATOGRAPHY; CRYPTOCOCCUS; ENZYME ANALYSIS; ENZYME PURIFICATION; HUMAN; HYDROLYSIS; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; SWINE;

AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ACID PROTEASES; CASEINS; CLONING, MOLECULAR; CRYPTOCOCCUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MOLECULAR SEQUENCE DATA; PEPSIN A; SACCHAROMYCES CEREVISIAE; SUBSTRATE SPECIFICITY; SWINE;

CRYPTOCOCCUS SP. S-2; SUIDAE;

EID: 81255160845     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.07.013     Document Type: Article

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