Cloning, expression and purification of extracellular serine protease Esp, a biofilm-degrading enzyme, from Staphylococcus epidermidis

Journal of Applied Microbiology

Volumn 111, Issue 6, 2011, Pages 1406-1415

  Sugimoto, S ^a   Iwase, T ^a   Sato, F ^a   Tajima, A ^a   Shinji, H ^a   Mizunoe, Y ^a  

^a JIKEI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Biofilm(s); Biotechnology; Proteinase; Rapid methods; Staphylococci

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; BIOFILMS; BIOTECHNOLOGY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENES; RECOMBINANT PROTEINS;

BIOFILM(S); BIOTECHNOLOGICAL APPLICATIONS; DEGRADING ENZYMES; EXTRACELLULAR; PRODUCTION SYSTEM; PROTEINASE; RAPID METHOD; SERINE PROTEASE; STAPHYLOCOCCUS; STAPHYLOCOCCUS EPIDERMIDIS;

PURIFICATION;

CELL MEMBRANE PROTEIN; HEXAHISTIDINE; SERINE PROTEINASE; SIGNAL PEPTIDE; THERMOLYSIN;

BACTERIUM; BIOFILM; BIOTECHNOLOGY; CHROMATOGRAPHY; CLONE; ENZYME ACTIVITY; GENE EXPRESSION; NICKEL; PROTEIN; PURIFICATION; RECOMBINATION; SECRETION;

ARTICLE; BIOFILM; BIOTECHNOLOGY; BREVIBACILLUS; BREVIBACILLUS CHOSHINENSIS; CLONING; ENZYME PURIFICATION; ESCHERICHIA COLI; NONHUMAN; PROTEIN DEGRADATION; PROTEIN EXPRESSION; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS;

BREVIBACILLUS CHOSHINENSIS; ESCHERICHIA COLI; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS EPIDERMIDIS;

EID: 81255153660     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2011.05167.x     Document Type: Article

References (20)

1. van den Berg, B., Wain, R., Dobson, C.M. and Ellis, R.J. (2000) Macromolecular crowding perturbs protein refolding kinetics: implications for folding inside the cell. EMBO J 19, 3870-3875.
2. Conley, A.J., Joensuu, J.J., Richman, A. and Menassa, R. (2011) Protein body-inducing fusions for high-level production and purification of recombinant proteins in plants. Plant Biotechnol J 9, 419-433.
3. De Jesus, M. and Wurm, F.M. (2011) Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors. Eur J Pharm Biopharm 78, 184-188.
4. Drapeau, G.R. (1978) Role of a metalloprotease in activation of the precursor of staphylococcal protease. J Bacteriol 136, 607-613.
5. Dubin, G., Chmiel, D., Mak, P., Rakwalska, M., Rzychon, M. and Dubin, A. (2001) Molecular cloning and biochemical characterization of proteases from Staphylococcus epidermidis. Biol Chem 382, 1575-1582.
6. Georgiou, G. and Segatori, L. (2005) Preparative expression of secreted proteins in bacteria: status report and future prospects. Curr Opin Biotechnol 16, 538-545.
7. Iwase, T., Uehara, Y., Shinji, H., Tajima, A., Seo, H., Takada, K., Agata, T. and Mizunoe, Y. (2010) Staphylococcus epidermidis Esp inhibits Staphylococcus aureus biofilm formation and nasal colonization. Nature 465, 346-349.
8. Maehashi, K., Matano, M., Saito, M. and Udaka, S. (2010) Extracellular production of riboflavin-binding protein, a potential bitter inhibitor, by Brevibacillus choshinensis. Protein Expr Purif 71, 85-90.
9. Marie-Claire, C., Ruffet, E., Beaumont, A. and Roques, B.P. (1999) The prosequence of thermolysin acts as an intramolecular chaperone when expressed in trans with the mature sequence in Escherichia coli. J Mol Biol 285, 1911-1915.
10. Moon, J.L., Banbula, A., Oleksy, A., Mayo, J.A. and Travis, J. (2001) Isolation and characterization of a highly specific serine endopeptidase from an oral strain of Staphylococcus epidermidis. Biol Chem 382, 1095-1099.
11. Nemoto, T.K., Ohara-Nemoto, Y., Ono, T., Kobayakawa, T., Shimoyama, Y., Kimura, S. and Takagi, T. (2008) Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli. FEBS J 275, 573-587.
12. Ohara-Nemoto, Y., Ikeda, Y., Kobayashi, M., Sasaki, M., Tajika, S. and Kimura, S. (2002) Characterization and molecular cloning of a glutamyl endopeptidase from Staphylococcus epidermidis. Microb Pathog 33, 33-41.
13. Ohara-Nemoto, Y., Ono, T., Shimoyama, Y., Kimura, S. and Nemoto, T.K. (2008) Homologous and heterologous expression and maturation processing of extracellular glutamyl endopeptidase of Staphylococcus epidermidis. Biol Chem 389, 1209-1217.
14. Porro, D., Gasser, B., Fossati, T., Maurer, M., Branduardi, P., Sauer, M. and Mattanovich, D. (2011) Production of recombinant proteins and metabolites in yeasts: when are these systems better than bacterial production systems? Appl Microbiol Biotechnol 89, 939-948.
15. Reutimann, H., Sjöberg, B.M. and Holmgren, A. (1985) Bacteriophage T7 DNA polymerase: cloning and high-level expression. Proc Natl Acad Sci USA 82, 6783-6787.
16. Sørensen, H.P. and Mortensen, K.K. (2005) Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol 115, 113-128.
17. Sugimoto, S., Higashi, C., Saruwatari, K., Nakayama, J. and Sonomoto, K. (2007) A gram-negative characteristic segment in Escherichia coli DnaK is essential for the ATP-dependent cooperative function with the co-chaperones DnaJ and GrpE. FEBS Lett 581, 2993-2999.
18. Teufel, P. and Götz, F. (1993) Characterization of an extracellular metalloprotease with elastase activity from Staphylococcus epidermidis. J Bacteriol 175, 4218-4224.
19. Trowitzsch, S., Bieniossek, C., Nie, Y., Garzoni, F. and Berger, I. (2010) New baculovirus expression tools for recombinant protein complex production. J Struct Biol 172, 45-54.
20. Yashiro, K., Lowenthal, J.W., O'Neil, T.E., Ebisu, S., Takagi, H. and Moore, R.J. (2001) High-level production of recombinant chicken interferon-γ by Brevibacillus choshinensis. Protein Expr Purif 23, 113-120.