An Inter-Laboratory validation of a real time PCR assay to measure host excretion of bacterial pathogens, particularly of Mycobacterium bovis

PLoS ONE

Volumn 6, Issue 11, 2011, Pages

  Travis, Emma R ^a   Gaze, William H ^a   Pontiroli, Alessandra ^a   Sweeney, Francis P ^a   Porter, David ^a   Mason, Sam ^a   Keeling, Matthew J C ^a   Jones, Rebecca M ^b   Sawyer, Jason ^b   Aranaz, Alicia ^c   Rizaldos, Elena ^c   Cork, Jennifer ^b   Delahay, Richard J ^d   Wilson, Gavin J ^d   Hewinson, R Glyn ^b   Courtenay, Orin ^a   Wellington, Elizabeth M ^a  

^a UNIVERSITY OF WARWICK   (United Kingdom)

^b VETERINARY LABORATORIES AGENCY   (United Kingdom)

^c UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^d FOOD AND ENVIRONMENT RESEARCH AGENCY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL CELL; BACTERIAL VIRULENCE; BACTERIUM DETECTION; BOVINE TUBERCULOSIS; CATTLE DISEASE; CONTROLLED STUDY; DILUTION; DNA EXTRACTION; EXPERIMENTAL DESIGN; FECES ANALYSIS; HOST; LABORATORY TEST; MYCOBACTERIUM BOVIS; NONHUMAN; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY; ANIMAL; FECES; GENETICS; ISOLATION AND PURIFICATION; LABORATORY; LABORATORY DIAGNOSIS; METHODOLOGY; MICROBIOLOGY; MUSTELIDAE; STANDARD;

BACTERIA (MICROORGANISMS); BOS; BOVINAE; CORYNEBACTERINEAE; MYCOBACTERIUM BOVIS; TAXIDIINAE;

ANIMALS; FALSE NEGATIVE REACTIONS; FECES; LABORATORIES; MUSTELIDAE; MYCOBACTERIUM BOVIS; REAL-TIME POLYMERASE CHAIN REACTION;

EID: 81055145736     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0027369     Document Type: Article

References (27)

1. Dokoupil S, (1964) Survival Of M. tuberculosis in grass, soil, bedding in cow sheds and urine. Vedecke Prace Vyzkumneho Ustavu Veterinarniho Lekarstvi V Brne 3: 49-52.
2. Young Js, Gormley E, Wellington EMH, (2005) Molecular detection of Mycobacterium bovis and Mycobacterium bovis BCG (Pasteur) in soil. Appl Environ Microbiol 71: 1946-1952.
3. Genov I, (1965) The effects of certain physical and chemical agents on Mycobacterium tuberculosis. Veteriární Medicina Nauki Sofia 2: 97-107.
4. Maddock ECG, (1933) Studies on the survival time of the bovine tubercle bacillus in soil, soil and dung, in dung and on grass, with experiments on the preliminary treatment of infected organic matter and the cultivation of the organism. Epidemiology and Infection 33: 103-117.
5. Williams RS, Hoy WA, (1930) The viability Of B. tuberculosis (Bovinus) on pasture land, in stored faeces and In liquid manure. J Hyg 30: 413-419.
6. Sweeney FP, Courtenay O, Ul-Hassan A, Hibberd V, Reilly LA, et al. (2006) Immunomagnetic recovery of Mycobacterium bovis from naturally infected environmental samples. Letters in Applied Microbiology 43: 364-369.
7. Sweeney FP, Courtenay O, Hibberd V, Hewinson RG, Reilly LA, et al. (2007) Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation. Appl Environ Microbiol 73: 7471-7473.
8. De Lisle GW, Bengis RG, Schmitt SM, O'Brien DJ, (2002) Tuberculosis in free-ranging wildlife: detection, diagnosis and management. Revue Scientifique et Technique de l' Office International des Epizooties 21: 317-334.
9. Griffin JM, Williams DH, Kelly GE, Clegg TA, O'Boyle I, et al. (2005) The impact of badger removal on the control of tuberculosis in cattle herds in Ireland. Prev Vet Medicine 67: 237-266.
10. Bourne FJ, Donnelly C, Cox D, Gettinby G, Mcinerney J, et al. (2007) Bovine TB: the scientific evidence, a science base for a sustainable policy to control Tb in cattle, an epidemiological investigation into bovine Tuberculosis. Final report of the independent scientific group on cattle TB. Department for Environment, Food & Rural AffairsEditor London, UK.
11. Defra, (2005) Government strategic framework for the sustainable control of bovine tuberculosis (bTB) in Great Britain. DEFRA, Scottish Executive, Welsh Assembly Government.
12. Defra, (2010) Breakdown of bovine TB Expenditure from the England bTB programme budget (Except compensation for Scotland and Wales): 1998/99-2008/09**.
13. Michel AL, Muller B, Van Helden PD, (2010) Mycobacterium bovis at the animal-human interface: a problem, or not? Vet Microbiol 140: 371-381.
14. WHO, (2010) Statement- 3rd International Conference on neglected zoonotic diseases: community-based interventions for prevention and control of neglected tropical diseases. Geneva.
15. Michel AL, De Klerk L-M, Gey Van Pittius NC, Warren RM, Van Helden PD, (2007) Bovine Tuberculosis in african buffaloes: observations regarding Mycobacterium bovis shedding into water and exposure to environmental Mycobacteria. BMC Vet Res 3: 23.
16. Keet DF, Michel AL, Bengis RG, Becker P, Van Dyk DS, et al. (2010) Intradermal tuberculin testing of wild african lions (Panthera Leo) naturally exposed to infection with Mycobacterium bovis. Vet Microbiol 144: 384-391.
17. Cleaveland S, Mlengeya T, Kazwala RR, Michel A, Kaare MT, et al. (2005) Tuberculosis in Tanzanian wildlife. J Of Wildlife Diseases 41: 446-453.
18. O'Brien DJ, Schmitt SM, Fitzgerald SD, Berry DE, Hickling GJ, (2006) Managing the wildlife reservoir of Mycobacterium Bovis: the Michigan, USA, experience. Vet Microbiol 112: 313-323.
19. Courtenay O, Wellington EMH, (2008) Mycobacterium Bovis in the environment: towards our understanding of its biology. Bcva Cattle Practice 16: 122-126.
20. Courtenay O, Reilly LA, Sweeney FPS, Macdonald DW, Delahay RJ, et al. (2007) Performance of an environmental test to detect Mycobacterium bovis infection in badger social groups. Vet Record 161: 817-818.
21. Courtenay O, Reilly LA, Sweeney FPS, Hibberd V, Bryan S, et al. (2006) Is Mycobacterium Bovis in the environment important for the persistence of bovine tuberculosis? Biol Letters 2: 460-462.
22. Delahay RJ, Langton S, Smith GC, Clifton-Hadley RS, Cheeseman CL, (2000) The Spatio-Temporal distribution of Mycobacterium bovis (Bovine Tuberculosis) Infection in a high-density badger population. J Animal Ecol 69: 428-441.
23. Pontiroli A, Travis ERT, Sweeney FPS, Porter D, Gaze WH, et al. (2011) Pathogen quantitation in complex matrices: a multi-operator comparison of DNA extraction methods with a novel assessment of PCR inhibition. PLoS ONE 6: E17916.
24. Delahay RJ, Brown JA, Mallinson PJ, Spyvee PD, Handoll D, et al. (2000) The use of marked bait in studies of the territorial organization of the European badger (Meles meles). Mammal Review 30: 73-87.
25. Drewe JA, Tomlinson AJ, Walker NJ, Delahay RJ, (2010) Diagnostic accuracy and optimal use of three tests for Tuberculosis in live badgers. PLoS ONE 5 (6).
26. Artz RRE, Avery LM, Jones DL, Killham K, (2006) Potential pitfalls in the quantitative molecular detection of Escherichia coli O157: H7 in environmental matrices. Can J Microbiol 52: 482-488.
27. Cliftonhadley RS, Wilesmith JW, Stuart FA, (1993) Mycobacterium bovis in the European badger (Meles meles)- Epidemiologic findings in tuberculous badgers from a naturally infected population. Epidemiology and Infection 111: 9-19.