Using an adherent cell culture of the mouse subependymal zone to study the behavior of adult neural stem cells on a single-cell level

Nature Protocols

Volumn 6, Issue 12, 2011, Pages 1847-1859

  Ortega, Felipe ^a   Costa, Marcos R ^b   Simon Ebert, Tatiana ^a   Schroeder, Timm ^c   Götz, Magdalena ^a,c   Berninger, Benedikt ^a,c  

^a UNIVERSITY OF MUNICH   (Germany)

^b FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE   (Brazil)

^c INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GROWTH FACTOR; MITOGENIC AGENT;

ADHERENT CELL; ADULT ANIMAL; ADULT STEM CELL; ANIMAL CELL; ARTICLE; CELL DENSITY; CELL DIVISION; CELL FUNCTION; CELL ISOLATION; CELL LINE; CELL TRACKING; CONTROLLED STUDY; CULTURE MEDIUM; IMMUNOCYTOCHEMISTRY; MACROGLIA; MICROSCOPY; MOUSE; NERVE CELL CULTURE; NEURAL STEM CELL; NEUROBLAST; NONHUMAN; PRIORITY JOURNAL; PROGENY; SINGLE CELL ANALYSIS; SUBVENTRICULAR ZONE; TIME LAPSE IMAGING; VIDEORECORDING;

ANIMALS; BRAIN; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL DIVISION; CELL LINEAGE; GENES, REPORTER; MICE; MICE, INBRED C57BL; NEURAL STEM CELLS; SOFTWARE; TIME-LAPSE IMAGING; VIDEO RECORDING;

EID: 80955152015     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2011.404     Document Type: Article

References (46)

1. Kriegstein, A. & Alvarez-Buylla, A. The glial nature of embryonic and adult neural stem cells. Annu. Rev. Neurosci. 32, 149-184 (2009).
2. Beckervordersandforth, R. et al. In vivo fate mapping and expression analysis reveals molecular hallmarks of prospectively isolated adult neural stem cells. Cell Stem Cell 7, 744-758 (2010).
3. Doetsch, F., Caille, I., Lim, D.A., Garcia-Verdugo, J.M. & Alvarez-Buylla, A. Subventricular zone astrocytes are neural stem cells in the adult mammalian brain. Cell 97, 703-716 (1999). (Pubitemid 29278045)
4. Doetsch, F., Garcia-Verdugo, J.M. & Alvarez-Buylla, A. Regeneration of a germinal layer in the adult mammalian brain. Proc. Natl. Acad. Sci. USA 96, 11619-11624 (1999). (Pubitemid 29487430)
5. Pastrana, E., Cheng, L.C. & Doetsch, F. Simultaneous prospective purifcation of adult subventricular zone neural stem cells and their progeny. Proc. Natl. Acad. Sci. USA 106, 6387-6392 (2009).
6. Mirzadeh, Z., Merkle, F.T., Soriano-Navarro, M., Garcia-Verdugo, J.M. & Alvarez-Buylla, A. Neural stem cells confer unique pinwheel architecture to the ventricular surface in neurogenic regions of the adult brain. Cell Stem Cell 3, 265-278 (2008).
7. Doetsch, F., Petreanu, L., Caille, I., Garcia-Verdugo, J.M. & Alvarez-Buylla, A. EGF converts transit-amplifying neurogenic precursors in the adult brain into multipotent stem cells. Neuron 36, 1021-1034 (2002). (Pubitemid 36044505)
8. Reynolds, B.A. & Weiss, S. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255, 1707-1710 (1992).
9. Reynolds, B.A. & Rietze, R.L. Neural stem cells and neurospheres-re-evaluating the relationship. Nat. Methods 2, 333-336 (2005). (Pubitemid 41130977)
10. Rietze, R.L. & Reynolds, B.A. Neural stem cell isolation and characterization. Methods Enzymol. 419, 3-23 (2006). (Pubitemid 44820360)
11. Kuhn, H.G., Winkler, J., Kempermann, G., Thal, L.J. & Gage, F.H. Epidermal growth factor and fbroblast growth factor-2 have different effects on neural progenitors in the adult rat brain. J. Neurosci. 17, 5820-5829 (1997). (Pubitemid 27310497)
12. Pastrana, E., Silva-Vargas, V. & Doetsch, F. Eyes wide open: a critical review of sphere-formation as an assay for stem cells. Cell Stem Cell 8, 486-498 (2011).
13. Costa, M.R. et al. Continuous live imaging of adult neural stem cell division and lineage progression in vitro. Development 138, 1057-1068 (2011).
14. Schroeder, T. Long-term single-cell imaging of mammalian stem cells. Nat. Methods 8, S30-S35 (2011).
15. Price, J., Turner, D. & Cepko, C. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84, 156-160 (1987). (Pubitemid 17013593)
16. Williams, B.P., Read, J. & Price, J. The generation of neurons and oligodendrocytes from a common precursor cell. Neuron 7, 685-693 (1991).
17. Lim, D.A. & Alvarez-Buylla, A. Interaction between astrocytes and adult subventricular zone precursors stimulates neurogenesis. Proc. Natl. Acad. Sci. USA 96, 7526-7531 (1999). (Pubitemid 29299695)
18. Laywell, E.D., Rakic, P., Kukekov, V.G., Holland, E.C. & Steindler, D.A. Identifcation of a multipotent astrocytic stem cell in the immature and adult mouse brain. Proc. Natl. Acad. Sci. USA 97, 13883-13888 (2000).
19. Brill, M.S. et al. Adult generation of glutamatergic olfactory bulb interneurons. Nat. Neurosci. 12, 1524-1533 (2009).
20. Brill, M.S. et al. A dlx2-and pax6-dependent transcriptional code for periglomerular neuron specifcation in the adult olfactory bulb. J. Neurosci. 28, 6439-6452 (2008).
21. Qian, X., Goderie, S.K., Shen, Q., Stern, J.H. & Temple, S. Intrinsic programs of patterned cell lineages in isolated vertebrate CNS ventricular zone cells. Development 125, 3143-3152 (1998). (Pubitemid 28434413)
22. Shen, Q. et al. The timing of cortical neurogenesis is encoded within lineages of individual progenitor cells. Nat. Neurosci. 9, 743-751 (2006).
23. Ravin, R. et al. Potency and fate specifcation in CNS stem cell populations in vitro. Cell Stem Cell 3, 670-680 (2008).
24. Rieger, M.A., Hoppe, P.S., Smejkal, B.M., Eitelhuber, A.C. & Schroeder, T. Hematopoietic cytokines can instruct lineage choice. Science 325, 217-218 (2009).
25. Nolte, C. et al. GFAP promoter-controlled EGFP-expressing transgenic mice: a tool to visualize astrocytes and astrogliosis in living brain tissue. Glia 33, 72-86 (2001). (Pubitemid 32102818)
26. Westerlund, U. et al. Endoscopically harvested stem cells: a putative method in future autotransplantation. Neurosurgery 57, 779-784; discussion 779-784 (2005).
27. Moe, M.C. et al. Multipotent progenitor cells from the adult human brain: neurophysiological differentiation to mature neurons. Brain 128, 2189-2199 (2005). (Pubitemid 41253285)
28. Sanai, N. et al. Unique astrocyte ribbon in adult human brain contains neural stem cells but lacks chain migration. Nature 427, 740-744 (2004).
29. Merkle, F.T., Mirzadeh, Z. & Alvarez-Buylla, A. Mosaic organization of neural stem cells in the adult brain. Science 317, 381-384 (2007). (Pubitemid 47106379)
30. Hack, M.A. et al. Neuronal fate determinants of adult olfactory bulb neurogenesis. Nat. Neurosci. 8, 865-872 (2005).
31. Morshead, C.M. et al. Neural stem cells in the adult mammalian forebrain: a relatively quiescent subpopulation of subependymal cells. Neuron 13, 1071-1082 (1994). (Pubitemid 24372126)
32. Zheng, W., Nowakowski, R.S. & Vaccarino, F.M. Fibroblast growth factor 2 is required for maintaining the neural stem cell pool in the mouse brain subventricular zone. Dev. Neurosci. 26, 181-196 (2004). (Pubitemid 40867974)
33. Cohen, A.R., Gomes, F.L., Roysam, B. & Cayouette, M. Computational prediction of neural progenitor cell fates. Nat. Methods 7, 213-218 (2010).
34. Tang, F., Lao, K. & Surani, M.A. Development and applications of single-cell transcriptome analysis. Nat. Methods 8, S6-S11 (2011).
35. Hirrlinger, P.G. et al. Expression of reef coral fuorescent proteins in the central nervous system of transgenic mice. Mol. Cell Neurosci. 30, 291-303 (2005). (Pubitemid 41416549)
36. Couillard-Despres, S. et al. Doublecortin expression levels in adult brain refect neurogenesis. Eur. J. Neurosci. 21, 1-14 (2005). (Pubitemid 40193870)
37. Couillard-Despres, S. et al. Targeted transgene expression in neuronal precursors: watching young neurons in the old brain. Eur. J. Neurosci. 24, 1535-1545 (2006). (Pubitemid 44427522)
38. Eilken, H. et al. Continuous long-term detection of live cell surface markers by 'in culture' antibody staining. Protocol Exchange (2011)doi:10.1038/protex.2011.205.
39. Bibel, M., Richter, J., Lacroix, E. & Barde, Y.A. Generation of a defned and uniform population of CNS progenitors and neurons from mouse embryonic stem cells. Nat. Protoc. 2, 1034-1043 (2007). (Pubitemid 46901284)
40. Chen, Y. et al. NS21: re-defned and modifed supplement B27 for neuronal cultures. J. Neurosci. Methods 171, 239-247 (2008).
41. Ory, D.S., Neugeboren, B.A. & Mulligan, R.C. A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes. Proc. Natl. Acad. Sci. USA 93, 11400-11406 (1996). (Pubitemid 26347133)
42. Heinrich, C. et al. Generation of subtype-specifc neurons from postnatal astroglia of the mouse cerebral cortex. Nat. Protoc. 6, 214-228 (2011).
43. Tashiro, A., Zhao, C. & Gage, F.H. Retrovirus-mediated single-cell gene knockout technique in adult newborn neurons in vivo. Nat. Protoc. 1, 3049-3055 (2006).
44. Billon, N., Jolicoeur, C. & Raff, M. Generation and characterization of oligodendrocytes from lineage-selectable embryonic stem cells in vitro. Methods Mol. Biol. 330, 15-32 (2006).
45. Costa, M.R., Bucholz, O., Schroeder, T. & Gotz, M. Late origin of glia-restricted progenitors in the developing mouse cerebral cortex. Cereb. Cortex 19 (Suppl 1): i135-i143 (2009).
46. Costa, M.R., Wen, G., Lepier, A., Schroeder, T. & Gotz, M. Par-complex proteins promote proliferative progenitor divisions in the developing mouse cerebral cortex. Development 135, 11-22 (2008). (Pubitemid 351201477)