Generation of a defined and uniform population of CNS progenitors and neurons from mouse embryonic stem cells

Nature Protocols

Volumn 2, Issue 5, 2007, Pages 1034-1043

  Bibel, Miriam ^a   Richter, Jens ^a   Lacroix, Emmanuel ^a   Barde, Yves Alain ^b  

^a NOVARTIS PHARMA AG   (Switzerland)

^b UNIVERSITY OF BASEL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTAMIC ACID; RETINOIC ACID; TRANSCRIPTION FACTOR PAX6;

ANIMAL CELL; ARTICLE; BRAIN NERVE CELL; CELL AGGREGATION; CELL MUTANT; CELL POPULATION; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; GLIA CELL; IN VIVO STUDY; MOUSE; NERVE CELL CULTURE; NERVE CELL DIFFERENTIATION; NEURAL STEM CELL; NONHUMAN; PRIORITY JOURNAL; PYRAMIDAL NERVE CELL; SYNAPTIC TRANSMISSION; WILD TYPE;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CENTRAL NERVOUS SYSTEM; EMBRYONIC STEM CELLS; MICE; NEURONS; TRETINOIN;

EID: 34250192881     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.147     Document Type: Article

References (17)

1. Greene, L.A. & Tischler, A.S. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73, 2424-2428 (1976).
2. Bain, G., Kitchens, D., Yao, M., Huettner, J.E. & Gottlieb, D.I. Embryonic stem cells express neuronal properties in vitro. Dev. Biol. 168, 342-357 (1995).
3. Li, M., Pevny, L., Lovell-Badge, R. & Smith, A. Generation of purified neural precursors from embryonic stem cells by lineage selection. Curr. Biol. 8, 971-974 (1998).
4. Okabe, S., Forsberg-Nilsson, K., Spiro, A.C., Segal, M. & McKay, R.O. Development of neuronal precursor cells and functional postmitotic neurons from embryonic stem cells in vitro. Mech. Dev. 59, 89-102 (1996).
5. Rathjen, J. & Rathjen, P.D. Mouse ES cells: Experimental exploitation of pluripotent differentiation potential. Curr. Opin. Genet. Dev. 11, 587-594 (2001).
6. Smith, A.G. Embryo-derived stem cells: Of mice and men. Annu. Rev. Cell Dev. Biol. 17, 435-462 (2001).
7. Wichterle, H., Lieberam, I., Porter, J.A. & Jessell, T.M. Directed differentiation of embryonic stem cells into motor neurons. Cell 110, 385-397 (2002).
8. Kim, J.Y. et al. Dopaminergic neuronal differentiation from rat embryonic neural precursors by Nurr1 overexpression. J. Neurochem. 85, 1443-1454 (2003).
9. Wobus, A.M. & Boheler, K.R. Embryonic stem cells: Prospects for developmental biology and cell therapy. Physiol. Rev. 85, 635-678 (2005).
10. Conti, L. et al. Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biol. 3, e283 (2005).
11. Keller, G. Embryonic stem cell differentiation: Emergence of a new era in biology and medicine. Genes Dev. 19, 1129-1155 (2005).
12. Bibel, M. et al. Differentiation of mouse embryonic stem cells into a defined neuronal lineage. Nat. Neurosci. 7, 1003-1009 (2004).
13. Malatesta, P. et al. Neuronal or glial progeny: Regional differences in radial glia fate. Neuron 37, 751-764 (2003).
14. Plachta, N., Bibel, M., Tucker, K.L. & Barde, Y.A. Developmental potential of defined neural progenitors derived from mouse embryonic stem cells. Development 131, 5449-5456 (2004).
15. Pera, M.F. & Trounson, A.O. Human embryonic stem cells: Prospects for development. Development 131, 5515-5525 (2004).
16. Pfaffl, M. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29, 2002-2007 (2001).
17. Tucker, K.L., Meyer, M. & Barde, Y.A. Neurotrophins are required for nerve growth during development. Nat. Neurosci. 4, 29-37 (2001).