Construct optimization for studying protein complexes: Obtaining diffraction-quality crystals of the pseudosymmetric PSPC1-NONO heterodimer

Acta Crystallographica Section D: Biological Crystallography

Volumn 67, Issue 11, 2011, Pages 981-987

  Lee, Mihwa ^a   Passon, Daniel M ^a   Hennig, Sven ^a   Fox, Archa H ^a   Bond, Charles S ^a  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

coiled coils; construct design; paraspeckles; pseudosymmetry; PSPC1 NONO heterodimer; RRM

Indexed keywords

MULTIPROTEIN COMPLEX; NONO PROTEIN, HUMAN; NUCLEAR MATRIX PROTEIN; NUCLEAR PROTEIN; OCTAMER TRANSCRIPTION FACTOR; PSPC1 PROTEIN, HUMAN; RNA BINDING PROTEIN;

ARTICLE; BIOLOGY; CELL NUCLEUS INCLUSION BODY; CHEMISTRY; CRYSTALLIZATION; GENE EXPRESSION REGULATION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; PROTEIN MULTIMERIZATION; SOLUBILITY; STEREOISOMERISM; X RAY CRYSTALLOGRAPHY;

COMPUTATIONAL BIOLOGY; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; GENE EXPRESSION REGULATION; HUMANS; INTRANUCLEAR INCLUSION BODIES; MULTIPROTEIN COMPLEXES; NUCLEAR MATRIX-ASSOCIATED PROTEINS; NUCLEAR PROTEINS; OCTAMER TRANSCRIPTION FACTORS; PROTEIN MULTIMERIZATION; RNA-BINDING PROTEINS; SOLUBILITY; STEREOISOMERISM;

EID: 80955123973     PISSN: 09074449     EISSN: 13990047     Source Type: Journal    
DOI: 10.1107/S0907444911039606     Document Type: Article

References (26)

1. Berger, B., Wilson, D. B., Wolf, E., Tonchev, T., Milla, M. & Kim, P. S. (1995). Proc. Natl Acad. Sci. USA, 92, 8259-8263.
2. Bond, C. S. & Fox, A. H. (2009). J. Cell Biol. 186, 637-644.
3. Bond, C. S. & Schüttelkopf, A. W. (2009). Acta Cryst. D65, 510-512.
4. Bryson, K., McGuffin, L. J., Marsden, R. L., Ward, J. J., Sodhi, J. S. & Jones, D. T. (2005). Nucleic Acids Res. 33, W36-W38.
5. Chen, L. L. & Carmichael, G. G. (2009). Mol. Cell, 35, 467-478.
6. Chen, L. L., DeCerbo, J. N. & Carmichael, G. G. (2008). EMBO J. 27, 1694-1705.
7. Clemson, C. M., Hutchinson, J. N., Sara, S. A., Ensminger, A. W., Fox, A. H., Chess, A. & Lawrence, J. B. (2009). Mol. Cell, 33, 717-726.
8. Dosztányi, Z., Csizmok, V., Tompa, P. & Simon, I. (2005). Bioinformatics, 21, 3433-3434.
9. Evans, P. (2006). Acta Cryst. D62, 72-82.
10. Finn, R. D., Mistry, J., Tate, J., Coggill, P., Heger, A., Pollington, J. E., Gavin, O. L., Gunasekaran, P., Ceric, G., Forslund, K., Holm, L., Sonnhammer, E. L., Eddy, S. R. & Bateman, A. (2010). Nucleic Acids Res. 38, D211-D222.
11. Fox, A. H., Bond, C. S. & Lamond, A. I. (2005). Mol. Biol. Cell, 16, 5304-5315.
12. Fox, A. H., Lam, Y. W., Leung, A. K., Lyon, C. E., Andersen, J., Mann, M. & Lamond, A. I. (2002). Curr. Biol. 12, 13-25.
13. Fox, A. H. & Lamond, A. I. (2010). Cold Spring Harb. Perspect. Biol. 2, a000687.
14. Kabsch, W. (2010). Acta Cryst. D66, 125-132.
15. Kiesler, E., Miralles, F., Ostlund Farrants, A. K. & Visa, N. (2003). J. Cell Sci. 116, 3949-3956.
16. Lupas, A., Van Dyke, M. & Stock, J. (1991). Science, 252, 1162-1164.
17. Mao, Y. S., Sunwoo, H., Zhang, B. & Spector, D. L. (2011). Nature Cell Biol. 13, 95-101.
18. Maris, C., Dominguez, C. & Allain, F. H. (2005). FEBS J. 272, 2118-2131.
19. McCoy, A. J., Grosse-Kunstleve, R. W., Adams, P. D., Winn, M. D., Storoni, L. C. & Read, R. J. (2007). J. Appl. Cryst. 40, 658-674.
20. Myojin, R., Kuwahara, S., Yasaki, T., Matsunaga, T., Sakurai, T., Kimura, M., Uesugi, S. & Kurihara, Y. (2004). Biol. Reprod. 71, 926-932.
21. Passon, D. M., Lee, M., Fox, A. H. & Bond, C. S. (2011). Acta Cryst. F67, 1231-1234.
22. Peng, R., Dye, B. T., Pérez, I., Barnard, D. C., Thompson, A. B. & Patton, J. G. (2002). RNA, 8, 1334-1347.
23. Prasanth, K. V., Prasanth, S. G., Xuan, Z., Hearn, S., Freier, S. M., Bennett, C. F., Zhang, M. Q. & Spector, D. L. (2005). Cell, 123, 249-263.
24. Sasaki, Y. T. & Hirose, T. (2009). Genome Biol. 10, 227.
25. Winn, M. D. et al. (2011). Acta Cryst. D67, 235-242.
26. Zhang, Z. & Carmichael, G. G. (2001). Cell, 106, 465-475.