The effects of fibroblast growth factor-21 knockdown and over-expression on its signaling pathway and glucose-lipid metabolism in vitro

Molecular and Cellular Endocrinology

Volumn 348, Issue 1, 2012, Pages 21-26

  Li, Ke ^a   Li, Ling ^a   Yang, Mengliu ^a   Liu, Hua ^b   Boden, Guenther ^c   Yang, Gangyi ^a  

^a CHONGQING MEDICAL UNIVERSITY   (China)

^b UNIVERSITY OF MISSISSIPPI SCHOOL OF MEDICINE   (United States)

^c TEMPLE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Fibroblast growth factor 21; Metabolism; RNA interference; Transcription factors

Indexed keywords

FIBROBLAST GROWTH FACTOR 21; MESSENGER RNA; SHORT HAIRPIN RNA; TRANSCRIPTION FACTOR; TRIACYLGLYCEROL;

ADIPOCYTE; ANIMAL CELL; AP2 GENE; ARTICLE; ATGL GENE; BETA KLOTHO GENE; CONTROLLED STUDY; FGFR1 GENE; FGFR4 GENE; FIBROBLAST GROWTH FACTOR 21 GENE; GENE; GENE OVEREXPRESSION; GENE SILENCING; GENE VECTOR; GLUCOSE METABOLISM; GLUCOSE TRANSPORT; GLUT 1 GENE; HMGR GENE; HSL GENE; IRS 1 GENE; LDLR GENE; LIPID METABOLISM; LIVER CELL; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPCK GENE; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; UPREGULATION;

3T3-L1 CELLS; ADIPOCYTES; ANIMALS; CHOLESTEROL; FIBROBLAST GROWTH FACTORS; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE KNOCKDOWN TECHNIQUES; GLUCONEOGENESIS; GLUCOSE; HEPATOCYTES; LIPID MOBILIZATION; MICE; SIGNAL TRANSDUCTION; TRANSCRIPTION FACTORS; TRIGLYCERIDES;

EID: 80755189417     PISSN: 03037207     EISSN: 18728057     Source Type: Journal    
DOI: 10.1016/j.mce.2011.07.026     Document Type: Article

References (21)

1. Blagoev B., Kratchmarova I., Nielsen M.M., Fernandez M.M., Voldby J., Andersen J.S. Inhibition of adipocyte differentiation by resistin-like molecule alpha. Biochemical characterization of its oligomeric nature. J. Biol. Chem. 2002, 277:42011-42016.
2. Coskun T., Bina H.A., Schneider M.A., Dunbar J.D., Hu C.C., Chen Y., Moller D.E., Kharitonenkov A. Fibroblast growth factor 21 corrects obesity in mice. Endocrinology 2008, 149:6018-6027.
3. Dushay J., Chui P.C., Gopalakrishnan G.S., Varela-Rey M., Crawley M., Fisher F.M., Badman M.K., Martinez-Chantar M.L., Maratos-Flier E. Increased fibroblast growth factor 21 in obesity and nonalcoholic fatty liver disease. Gastroenterology 2010, 139:456-463.
4. Fisher F.M., Chui P.C., Antonellis P.J., Bina H.A., Kharitonenkov A., Flier J.S., Maratos-Flier E. Obesity is a fibroblast growth factor 21 (FGF21)-resistant state. Diabetes 2010, 59:2781-2789.
5. Hotta Y., Nakamura H., Konishi M., Murata Y., Takagi H., Matsumura S., Inoue K., Fushiki T., Itoh N. Fibroblast growth factor 21 regulates lipolysis in white adipose tissue but is not required for ketogenesis and triglyceride clearance in liver. Endocrinology 2009, 150:4625-4633.
6. Ito S., Kinoshita S., Shiraishi N., Nakagawa S., Sekine S., Fujimori T., Nabeshima Y.I. Molecular cloning and expression analyses of mouse βklotho, which encodes a novel Klotho family protein. Mech. Dev. 2000, 98:115-119.
7. Kharitonenkov A., Shiyanova T.L., Koester A., Ford A.M., Micanovic R., Galbreath E.J., Sandusky G.E., Hammond L.J., Moyers J.S., Owens R.A., Gromada J., Brozinick J.T., Hawkins E.D., Wroblewski V.J., Li D.S., Mehrbod F., Jaskunas S.R., Shanafelt A.B. FGF-21 as a novel metabolic regulator. J. Clin. Invest. 2005, 115:1627-1635.
8. Kharitonenkov A., Wroblewski V.J., Koester A., Chen Y.F., Clutinger C.K., Tigno X.T., Hansen B.C., Shanafelt A.B., Etgen G.J. The metabolic state of diabetic monkeys is regulated by fibroblast growth factor-21. Endocrinology 2007, 148:774-781.
9. Kurosu H., Choi M., Ogawa Y., Dickson A.S., Goetz R., Eliseenkova A.V., Mohammadi M., Rosenblatt K.P., Kliewer S.A., Kuro-o M. Tissue-specific expression of βKlotho and fibroblast growth factor (FGF) receptor isoforms determines metabolic activity of FGF19 and FGF21. J. Biol. Chem. 2007, 282:26687-26695.
10. Langin D. Adipose tissue lipolysis as a metabolic pathway to define pharmacological strategies against obesity and the metabolic syndrome. Pharmacol. Res. 2006, 53:482-491.
11. Li K., Li L., Yang M., Zong H., Liu H., Yang G. Effects of rosiglitazone on fasting plasma fibroblast growth factor-21 levels in patients with type 2 diabetes mellitus. Eur. J. Endocrinol. 2009, 161:391-395.
12. Li L., Yang G., Heu N., Yang M., Liu H., Chen W. Plasma FGF-21 levels in type 2 diabetic patients with ketosis. Diabetes Res. Clin. Pract. 2008, 82:209-213.
13. Nishimura T., Nakatake Y., Konishi M., Itoh N. Identification of a novel FGF, FGF-21, preferentially expressed in the liver. Biochim. Biophys. Acta 2000, 1492:203-206.
14. Ogawa Y., Kurosu H., Yamamoto M., Nandi A., Rosenblatt K.P., Goetz R., Eliseenkova A.V., Mohammadi M., Kuro-o M. βKlotho is required for metabolic activity of fibroblast growth factor 21. Proc. Natl. Acad. Sci. USA 2007, 104:7432-7437.
15. Suzuki M., Uehara Y., Motomura-Matsuzaka K., Oki J., Koyama Y., Kimura M., Asada M., Komi-Kuramochi A., Oka S., Imamura T. betaKlotho is required for fibroblast growth factor (FGF) 21 signaling through FGF receptor (FGFR) 1c and FGFR3c. Mol. Endocrinol. 2008, 22:1006-1014.
16. Watt M.J. Triglyceride lipases alter fuel metabolism and mitochondrial gene expression. Appl. Physiol. Nutr. Metab. 2009, 34:340-347.
17. Wente W., Efanov A.M., Brenner M., Kharitonenkov A., Köster A., Sandusky G.E., Sewing S., Treinies I., Zitzer H., Gromada J. Fibroblast growth factor-21 improves pancreatic beta-cell function and survival by activation of extracellular signal-regulated kinase 1/2 and Akt signaling pathways. Diabetes 2006, 55:2470-2478.
18. Xu J., Lloyd D.J., Hale C., Stanislaus S., Chen M., Sivits G., Vonderfecht S., Hecht R., Li Y.S., Lindberg R.A., Chen J.L., Jung D.Y., Zhang Z., Ko H.J., Kim J.K., Véniant M.M. Fibroblast growth factor 21 reverses hepatic steatosis, increases energy expenditure, and improves insulin sensitivity in diet-induced obese mice. Diabetes 2009, 58:250-259.
19. Yilmaz Y., Eren F., Yonal O., Kurt R., Aktas B., Celikel C.A., Ozdogan O., Imeryuz N., Kalayci C., Avsar E. Increased serum FGF21 levels in patients with nonalcoholic fatty liver disease. Eur. J. Clin. Invest. 2010, 40:887-892.
20. Zhang X., Ibrahimi O.A., Olsen S.K., Umemori H., Mohammadi M., Ornitz D.M. Receptor specificity of the fibroblast growth factor family. The complete mammalian FGF family. J. Biol. Chem. 2006, 281:15694-15700.
21. Zimmermann R., Strauss J.G., Haemmerle G., Schoiswohl G., Birner-Gruenberger R., Riederer M., Lass A., Neuberger G., Eisenhaber F., Hermetter A., Zechner R. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase. Science 2004, 306:1383-1386.