Production of N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) fused with secretory signal Igκ in insect cells

Protein Expression and Purification

Volumn 81, Issue 2, 2012, Pages 175-180

  Horynová, Milada ^a,b   Takahashi, Kazuo ^b   Hall, Stacy ^b   Renfrow, Matthew B ^c   Novak, Jan ^b   Raška, Milan ^a,b  

^a PALACKÝ UNIVERSITY   (Czech Republic)

^b University of Alabama at Birmingham   (United States)

^c UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

Baculovirus expression system; Immunoglobulin A1 (IgA1); Immunoglobulin light chain secretory signal; UDP N acetyl d galactosamine:polypeptide N acetylgalactosaminyl transferase 2 (GalNAc T2)

Indexed keywords

HYBRID PROTEIN; IMMUNOGLOBULIN A; IMMUNOGLOBULIN KAPPA CHAIN; N ACETYLGALACTOSAMINYLTRANSFERASE; POLYPEPTIDE N ACETYLGALACTOSAMINYLTRANSFERASE; POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE; SIGNAL PEPTIDE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BACULOVIRUS; BIOSYNTHESIS; CELL STRAIN HEK293; CHEMISTRY; COMPARATIVE STUDY; CULTURE MEDIUM; ENZYME ACTIVATION; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HUMAN; INSECT; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOUSE; PLASMID; PROTEIN STABILITY; SOLUBILITY; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANIMALS; BACULOVIRIDAE; CLONING, MOLECULAR; CULTURE MEDIA; ENZYME ACTIVATION; GENETIC VECTORS; HEK293 CELLS; HUMANS; IMMUNOGLOBULIN A; IMMUNOGLOBULIN KAPPA-CHAINS; INSECTS; MICE; MOLECULAR SEQUENCE DATA; N-ACETYLGALACTOSAMINYLTRANSFERASES; PLASMIDS; PROTEIN SORTING SIGNALS; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TANDEM MASS SPECTROMETRY; TRANSFECTION;

HEXAPODA; MAMMALIA; MURINAE;

EID: 80755184680     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.10.006     Document Type: Article

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