Identification of spermatogenesis with multiphoton microscopy: An evaluation in a rodent model

Journal of Urology

Volumn 186, Issue 6, 2011, Pages 2487-2492

  Ramasamy, Ranjith ^a   Sterling, Joshua ^a   Fisher, Erik S ^a   Li, Philip S ^a   Jain, Manu ^a   Robinson, Brian D ^a   Shevchuck, Maria ^a   Huland, David ^b   Xu, Chris ^b   Mukherjee, Sushmita ^a   Schlegel, Peter N ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

^b Cornell University   (United States)

Author keywords

fluorescence; infertility; male; microscopy; multiphoton; seminiferous tubules; spermatogenesis; testis

Indexed keywords

DNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; AUTOFLUORESCENCE IMAGING; AZOOSPERMIA; BIOLOGICAL PHENOMENA AND FUNCTIONS CONCERNING THE ENTIRE ORGANISM; CLINICAL DECISION MAKING; CONTROLLED STUDY; DNA DAMAGE; DNA FRAGMENTATION; HISTOPATHOLOGY; MALE; MULTIPHOTON MICROSCOPY; NEWBORN; NICK END LABELING; NONHUMAN; PRIORITY JOURNAL; RAT; SEMINIFEROUS TUBULE; SERTOLI CELL; SPECTROSCOPY; SPERMATOGENESIS; TESTICULAR SPERM EXTRACTION; TESTIS BIOPSY;

ANIMALS; MALE; MICROSCOPY; RATS; RATS, SPRAGUE-DAWLEY; SEMINIFEROUS TUBULES; SPERMATOGENESIS;

EID: 80755136617     PISSN: 00225347     EISSN: 15273792     Source Type: Journal    
DOI: 10.1016/j.juro.2011.07.081     Document Type: Article

References (23)

1. A. Tsujimura, K. Matsumiya, and Y. Miyagawa Conventional multiple or microdissection testicular sperm extraction: a comparative study Hum Reprod 17 2002 2924
2. P. Thonneau, S. Marchand, and A. Tallec Incidence and main causes of infertility in a resident population (1,850,000) of three French regions (1988-1989) Hum Reprod 6 1991 811
3. P.N. Schlegel Testicular sperm extraction: microdissection improves sperm yield with minimal tissue excision Hum Reprod 14 1999 131
4. T. Ishikawa, R. Nose, and K. Yamaguchi Learning curves of microdissection testicular sperm extraction for nonobstructive azoospermia Fertil Steril 94 2010 1008
5. R. Ramasamy, N. Yagan, and P.N. Schlegel Structural and functional changes to the testis after conventional versus microdissection testicular sperm extraction Urology 65 2005 1190
6. H. Okada, M. Dobashi, and T. Yamazaki Conventional versus microdissection testicular sperm extraction for nonobstructive azoospermia J Urol 168 2002 1063
7. A.M. Andersson, N. Jorgensen, and L. Frydelund-Larsen Impaired Leydig cell function in infertile men: a study of 357 idiopathic infertile men and 318 proven fertile controls J Clin Endocrinol Metab 89 2004 3161
8. J.H. MacIndoe The challenges of testosterone deficiency Uncovering the problem, evaluating the role of therapy Postgrad Med 114 2003 51
9. T. Ishikawa, K. Yamaguchi, and K. Chiba Serum hormones in patients with nonobstructive azoospermia after microdissection testicular sperm extraction J Urol 182 2009 1495
10. S. Takada, A. Tsujimura, and T. Ueda Androgen decline in patients with nonobstructive azoospermia after microdissection testicular sperm extraction Urology 72 2008 114
11. S. Mukherjee, J.S. Wysock, and C.K. Ng Human bladder cancer diagnosis using Multiphoton microscopy Proc Soc Photo Opt Instrum Eng 7161 2009 nihpa96839
12. J.N. Rogart, J. Nagata, and C.S. Loeser Multiphoton imaging can be used for microscopic examination of intact human gastrointestinal mucosa ex vivo Clin Gastroenterol Hepatol 6 2008 95
13. C. Xu, W. Zipfel, and J.B. Shear Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy Proc Natl Acad Sci U S A 93 1996 10763
14. R. Yadav, S. Mukherjee, and M. Hermen Multiphoton microscopy of prostate and periprostatic neural tissue: a promising imaging technique for improving nerve-sparing prostatectomy J Endourol 23 2009 861
15. W.R. Zipfel, R.M. Williams, and R. Christie Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation Proc Natl Acad Sci U S A 100 2003 7075
16. R. Christie, E. Kimchi, and S. Kajdasz Multiphoton microscopy and amyloid angiopathy Amyloid 8 suppl 2001 48
17. D.A. Dombeck, K.A. Kasischke, and H.D. Vishwasrao Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy Proc Natl Acad Sci U S A 100 2003 7081
18. M.J. Levene, D.A. Dombeck, and K.A. Kasischke In vivo multiphoton microscopy of deep brain tissue J Neurophysiol 91 2004 1908
19. M.J. Koehler, S. Zimmermann, and S. Springer Keratinocyte morphology of human skin evaluated by in vivo multiphoton laser tomography Skin Res Technol 2011 Epub ahead of print
20. E.A. Gibson, O. Masihzadeh, and T.C. Lei Multiphoton microscopy for ophthalmic imaging J Ophthalmol 2011 2011 870879
21. R.G. Nava, W. Li, and A.E. Gelman Two-photon microscopy in pulmonary research Semin Immunopathol 32 2010 297
22. G.P. Young, M. Goldstein, and D.M. Phillips Sertoli cell-only syndrome produced by cold testicular ischemia Endocrinology 122 1988 1074
23. N. Kotaja, S. Kimmins, and S. Brancorsini Preparation, isolation and characterization of stage-specific spermatogenic cells for cellular and molecular analysis Nat Methods 1 2004 249