Preparation, isolation and characterization of stage-specific spermatogenic cells for cellular and molecular analysis

Nature Methods

Volumn 1, Issue 3, 2004, Pages 249-254

  Kotaja, Noora ^a   Kimmins, Sarah ^a   Brancorsini, Stefano ^a   Hentsch, Didier ^a   Vonesch, Jean Luc ^a   Davidson, Irwin ^a   Parvinen, Martti ^b   Sassone Corsi, Paolo ^a  

^a INSTITUT DE GÉNÉTIQUE ET DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

^b UNIVERSITY OF TURKU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; ARTICLE; CELL SEPARATION; CYTOLOGY; GENETICS; MALE; METHODOLOGY; MOUSE; PHYSIOLOGY; SEMINIFEROUS TUBULE; SPERMATID; SPERMATOCYTE; SPERMATOGENESIS; SPERMATOGONIUM; SPERMATOZOON;

ANIMALS; CELL SEPARATION; MALE; MICE; SEMINIFEROUS TUBULES; SPERMATIDS; SPERMATOCYTES; SPERMATOGENESIS; SPERMATOGONIA; SPERMATOZOA;

EID: 11944258521     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth1204-249     Document Type: Article

References (20)

1. Sassone-Corsi, P. Unique chromatin remodeling and transcriptional regulation in spermatogenesis. Science 296, 2176–2178 (2002).
2. Russell, L.D., Ettlin, R.A., SinhaHikim, A.P. & Clegg, E.D. Mammalian Spermatogenesis in Histological and Histopathological Evaluation of the Testis pp. 1– 40 (Cache River Press, St. Louis, 1990).
3. Perey, B., Clermont, Y. & Leblond, C.P. The wave of the seminiferous epithelium in the rat. Am. J. Anat. 108, 47–77 (1961).
4. Parvinen, M. & Vanha-Perttula, T. Identification and enzyme quantitation of the stages of the seminiferous epithelial wave in the rat. Anat. Rec. 174, 435–450 (1972).
5. Toppari, J., Eerola, E. & Parvinen, M. Flow cytometric DNA analysis of defined stages of rat seminiferous epithelial cycle during in vitro differentiation. J. Androl. 6, 325–333 (1985).
6. Toppari, J. & Parvinen, M. In vitro differentiation of rat seminiferous tubular segments from defined stages of the epithelial cycle morphologic and immunolocalization analysis. J. Androl. 6, 334–343 (1985).
7. Leblond, C.P. & Clermont, Y. Definition of the stages of the cycle of the seminiferous epithelium in the rat. Ann. NY Acad. Sci. 55, 548–573 (1952).
8. Parvinen, M. Regulation of the seminiferous epithelium. Endocr. Rev. 3, 404–417 (1982).
9. Toppari, J., Bishop, P.C., Parker, J.W. & diZerega, G.S. DNA flow cytometric analysis of mouse seminiferous epithelium. Cytometry 9, 456–462 (1988).
10. Kangasniemi, M. et al. Modulation of basal and FSHdependent cyclic AMP production in rat seminiferous tubules staged by an improved transillumination technique. Anat. Rec. 227, 62–76 (1990).
11. Parvinen, M., Toppari, J. & Lähdetie J. Transilluminationphase contrast microscopic techniques for evaluation of male germ cell toxicity and mutagenicity. in Methods in Reproductive Toxicology (R.E. Chapin & J. Heindel, eds.) 142–165 (Academic Press, Orlando, Florida, USA, 1993).
12. Parvinen, M. & Hecht, N.B. Identification of living spermatogenic cells of the mouse by transillumination-phase contrast microscopic technique for ‘in situ’ analyses of DNA polymerase activities. Histochemistry 71, 567–579 (1981).
13. Peters, A.H., Plug, A.W., van Vugt, M.J. & de Boer, P. A drying-down technique for the spreading of mammalian meiocytes from the male and female germline. Chromosome Res. 5, 66–68 (1997).
14. Henriksen, K. & Parvinen, M. Stage-specific apoptosis of male germ cells in the rat: Mechanisms of cell death studied by supravital squash preparations. Tissue Cell 30, 692–701 (1998).
15. Ventelä, S., Toppari, J. & Parvinen, M. Intercellular organelle traffic through cytoplasmic bridges in early spermatids of the rat: Mechanisms of haploid gene product sharing. Mol. Biol. Cell 14, 2768–2780 (2003).
16. Macho, B. et al. CREM-dependent transcription in male germ cells controlled by a kinesin. Science 298, 2388–2390 (2002).
17. De Cesare, D., Fimia, G.M., Brancorsini, S., Parvinen, M. & Sassone-Corsi, P. Transcriptional control in male germ cells: General factor TFIIA participates in CREM-dependent gene activation. Mol. Endocrinol. 17, 2554–2565 (2003).
18. Meng, X. et al. Regulation of cell fate decision of undifferentiated spermatogonia by GDNF. Science 287, 1489–1493 (2000).
19. Martianov, I. et al. Late arrest of spermiogenesis and germ cell apoptosis in mice lacking the TBP-like TLF/TRF2 gene. Mol. Cell 7, 509–515 (2001).
20. Martianov, I. et al. Distinct functions of TBP and TLF/TRF2 during spermatogenesis: Requirement of TLF for heterochromatic chromocenter formation in haploid round spermatids. Development 129, 945–955 (2002).