Sulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay

Analytical Biochemistry

Volumn 417, Issue 1, 2011, Pages 156-158

  Vashist, Sandeep Kumar ^a   Zhang, Binbin ^a   Zheng, Dan ^a   Al Rubeaan, Khalid ^b   Luong, John H T ^a,c   Sheu, Fwu Shan ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b KING SAUD UNIVERSITY   (Saudi Arabia)

^c NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; MAMMALS; REAGENTS; SULFUR COMPOUNDS;

ABSORBANCE PEAK; ABSORBANCES; BICINCHONINIC ACIDS; BIO-CONJUGATION; BOVINE SERUM ALBUMINS; CUPRIC SULFATE; N-HYDROXYSUCCINIMIDE; PROTEIN ASSAYS;

PROTEINS;

BICINCHONINIC ACID; CINCHONINE; COPPER; COPPER SULFATE; SUCCINIMIDE DERIVATIVE; SULFO N HYDROXYSUCCINIMIDE; UNCLASSIFIED DRUG; 1 (3 DIMETHYLAMINOPROPYL) 3 ETHYLCARBODIIMIDE; BOVINE SERUM ALBUMIN; N HYDROXYSUCCINIMIDE; N-HYDROXYSUCCINIMIDE; QUINOLINE DERIVATIVE;

ABSORPTION; ANALYTICAL PARAMETERS; ARTICLE; CONCENTRATION (PARAMETERS); CONJUGATION; PRIORITY JOURNAL; PROTEIN DETERMINATION; ANIMAL; BIOASSAY; CATTLE; CHEMISTRY; METHODOLOGY;

BOVINAE;

ANIMALS; BIOLOGICAL ASSAY; CATTLE; ETHYLDIMETHYLAMINOPROPYL CARBODIIMIDE; QUINOLINES; SERUM ALBUMIN, BOVINE; SUCCINIMIDES;

EID: 80655148685     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.05.045     Document Type: Article

References (20)

1. P.K. Smith, R.I. Krohn, G.T. Hermanson, A.K. Mallia, F.H. Gartner, M.D. Provenzano, E.K. Fujimoto, N.M. Goeke, B.J. Olson, D.C. Klenk, Measurement of protein using bicinchoninic acid, Anal. Biochem. 150 (1985) 76-85. (Pubitemid 16258399)
2. K.J. Wiechelman, R.D. Braun, J.D. Fitzpatrick, Investigation of the bicinchoninic acid protein assay: identification of the groups responsible for color formation, Anal. Biochem. 175 (1988) 231-237.
3. R.J. Kessler, D.D. Fanestil, Interference by lipids in the determination of protein using bicinchoninic acid, Anal. Biochem. 159 (1986) 138-142. (Pubitemid 17228236)
4. R.E. Brown, K.L. Jarvis, K.J. Hyland, Protein measurement using bicinchoninic acid: elimination of interfering substances, Anal. Biochem. 180 (1989) 136- 139. (Pubitemid 19200575)
5. J. Kang, X.-Y. Zhang, L.-D. Sun, X.-X. Zhang, Bioconjugation of functionalized fluorescent YVO4:Eu nanocrystals with BSA for immunoassay, Talanta 71 (2007) 1186-1191. (Pubitemid 46161642)
6. J. Lee, J. Yang, S.-B. Seo, H.-J. Ko, J.-S. Suh, Y.-M. Huh, S. Haam, Smart nanoprobes for ultrasensitive detection of breast cancer via magnetic resonance imaging, Nanotechnology 19 (2008) 485101.
7. J. Wan, X. Meng, E. Liu, K. Chen, Incorporation of magnetite nanoparticle clusters in fluorescent silica nanoparticles for high-performance brain tumor delineation, Nanotechnology 21 (2010) 235104.
8. Y. Kawai, H. Fujii, M. Okada, Y. Tsuchie, K. Uchida, T. Osaka, Formation of Ne- (succinyl)lysine in vivo: a novel marker for docosahexaenoic acid-derived protein modification, J. Lipid Res. 47 (2006) 1386-1398. (Pubitemid 44079892)
9. K.L. Lynch, R.R.L. Gerona, E.C. Larsen, R.F. Marcia, J.C. Mitchell, T.F.J. Martin, Synaptotagmin C2A Loop 2 mediates Ca2+-dependent SNARE Interactions essential for Ca2+-triggered vesicle exocytosis, Mol. Biol. Cell 18 (2007) 4957- 4968. (Pubitemid 350246696)
10. F.-Y. Chiu, Y.-R. Chen, S.-L. Tu, Electrostatic interaction of phytochromobilin synthase and ferredoxin for biosynthesis of phytochrome chromophore, J. Biol. Chem. 285 (2010) 5056-5065.
11. S. Yamashiro, K.D. Speicher, D.W. Speicher, V.M. Fowler, Mammalian tropomodulins nucleate actin polymerization via their actin monomer binding and filament pointed end-capping activities, J. Biol. Chem. 285 (2010) 33265-33280.
12. I.Y. Churbanova, T.L. Poulos, I.F. Sevrioukova, Production and characterization of a functional putidaredoxin reductase-putidaredoxin covalent complex, Biochemistry 49 (2010) 58-67.
13. M.F. Zambaux, F. Bonneaux, E. Dellacherie, Covalent fixation of soluble derivatized dextrans to model proteins in low-concentration medium: application to Factor IX and protein C, J. Protein Chem. 17 (1998) 279-284. (Pubitemid 28194492)
14. G.T. Hermanson, Bioconjugate Techniques, second ed., Elsevier Academic Press, USA, 2009.
15. J.V. Staros, R.W. Wright, D.M. Swingle, Enhancement by Nhydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions, Anal. Biochem. 156 (1986) 220-222. (Pubitemid 16044423)
16. Z. Grabarek, J. Gergely, Zero-length crosslinking procedure with the use of active esters, Anal. Biochem. 185 (1990) 131-135. (Pubitemid 20082102)
17. C.K. Dixit, S.K. Vashist, F.T. O'Neill, B. O'Reilly, B.D. MacCraith, R. O'Kennedy, Development of a high sensitivity rapid sandwich ELISA procedure and its comparison with the conventional approach, Anal. Chem. 82 (2010) 7049- 7052.
18. C.K. Dixit, S.K. Vashist, B.D. MacCraith, R. O'Kennedy, Multi-substrate compatible ELISA procedures for rapid and high sensitivity immunoassays, Nat. Protoc. 6 (2011) 439-445.
19. T. Cass, F.S. Ligler, Immobilized Biomolecules in Analysis: a Practical Approach, Oxford Univ. Press, USA, 1998.
20. R. Kumar, A.K. Shukla, E. Bagga, S. Kumari, R.P. Bajpai, L.M. Bharadwaj, 1-Ethyl- 3-(3-dimethylaminopropyl) carbodiimide interference with Lowry method, Anal. Biochem. 336 (2005) 132-134. (Pubitemid 39593509)