Detection of vancomycin-resistant enterococci from faecal samples of Iberian wolf and Iberian lynx, including Enterococcus faecium strains of CC17 and the new singleton ST573

Science of the Total Environment

Volumn 410-411, Issue , 2011, Pages 266-268

  Gonçalves, Alexandre ^a,b   Igrejas, Gilberto ^a   Radhouani, Hajer ^a,b   López, María ^c   Guerra, Ana ^d   Petrucci Fonseca, Francisco ^d   Alcaide, Eva ^e   Zorrilla, Irene ^e   Serra, Rodrigo ^f   Torres, Carmen ^c   Poeta, Patrícia ^a,b  

^a UNIVERSITY OF TRÁS OS MONTES AND ALTO DOURO   (Portugal)

^b UNIVERSITY OF TRÁS OS MONTES AND ALTO DOURO   (Portugal)

^c UNIVERSITY OF LA RIOJA   (Spain)

^d UNIVERSITY OF LISBON   (Portugal)

^e JUNTA DE ANDALUCÍA   (Spain)

^f National Center for Captive Breeding of the Iberian Lynx   (Portugal)

Author keywords

Antibiotic resistance; Iberian lynx; Iberian wolf; VRE

Indexed keywords

ANTIBIOTIC RESISTANCE; CLONAL COMPLEX; ENTEROCOCCUS FAECIUM STRAINS; ERYTHROMYCIN RESISTANCE; FAECAL SAMPLES; GENES ENCODING; IBERIAN LYNX; IBERIAN WOLF; INTRINSIC RESISTANCE; KANAMYCINS; MOLECULAR CHARACTERIZATION; MULTILOCUS SEQUENCE TYPING; RESISTANCE GENES; VANCOMYCIN; VANCOMYCIN-RESISTANT ENTEROCOCCI; VIRULENCE FACTORS; VIRULENCE GENE; VRE;

ANTIBIOTICS; BACILLI; RECOVERY;

GENES;

AMPICILLIN; ERYTHROMYCIN; KANAMYCIN; TETRACYCLINE;

ANTIBIOTIC RESISTANCE; BACTERIUM; CANID; DETECTION METHOD; FECES; FELID; GENE; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; VIRULENCE;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CONTROLLED STUDY; ENTEROCOCCUS CASSELIFLAVUS; ENTEROCOCCUS FAECIUM; ENTEROCOCCUS GALLINARUM; FECES ANALYSIS; FECES MICROFLORA; GENE IDENTIFICATION; HYL GENE; LYNX; MULTIDRUG RESISTANCE; MULTILOCUS SEQUENCE TYPING; NONHUMAN; PRIORITY JOURNAL; VANA GENE; VANC1 GENE; VANCOMYCIN RESISTANT ENTEROCOCCUS; WOLF;

ANIMALS; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; ENTEROCOCCUS; ENTEROCOCCUS FAECIUM; FECES; LYNX; MULTILOCUS SEQUENCE TYPING; PORTUGAL; SPAIN; VANCOMYCIN; VANCOMYCIN RESISTANCE; VIRULENCE FACTORS; WOLVES;

ENTEROCOCCUS FAECIUM; LYNX;

EID: 80655133063     PISSN: 00489697     EISSN: 18791026     Source Type: Journal    
DOI: 10.1016/j.scitotenv.2011.09.074     Document Type: Article

References (18)

1. Aarestrup F.M., Agerso Y., Gerner-Smidt P., Madsen M., Jensen L.B. Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark. Diagn Microbiol Infect Dis 2000, 37:127-137.
2. Agerso Y., Pedersen A.G., Aarestrup F.M. Identification of Tn5397-like and Tn916-like transposons and diversity of the tetracycline resistance gene tet(M) in enterococci from humans, pigs and poultry. J Antimicrob Chemother 2006, 57:832-839.
3. Biavasco F., Foglia G., Paoletti C., Zandri G., Magi G., Guaglianone E., et al. VanA-type enterococci from humans, animals, and food: species distribution, population structure, Tn1546 typing and location, and virulence determinants. Appl Environ Microbiol 2007, 73:3307-3319.
4. CLSI Performance standards for antimicrobial susceptibility testing. Informational supplement. CLSI document M100-S20 2010, Clinical and Laboratory Standards Institute, Wayne, PA, USA. Twentieth edition.
5. Creti R., Imperi M., Bertuccini L., Fabretti F., Orefici G., Di Rosa R., et al. Survey for virulence determinants among Enterococcus faecalis isolated from different sources. J Med Microbiol 2004, 53:13-20.
6. De Leener E., Martel A., Decostere A., Haesebrouck F. Distribution of the erm (B) gene, tetracycline resistance genes, and Tn1545-like transposons in macrolide- and lincosamide-resistant enterococci from pigs and humans. Microb Drug Resist 2004, 10:341-345.
7. Eaton T.J., Gasson M.J. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl Environ Microbiol 2001, 67:1628-1635.
8. Figueiredo N., Radhouani H., Goncalves A., Rodrigues J., Carvalho C., Igrejas G., et al. Genetic characterization of vancomycin-resistant enterococci isolates from wild rabbits. J Basic Microbiol 2009, 49:491-494.
9. Homan W.L., Tribe D., Poznanski S., Li M., Hogg G., Spalburg E., et al. Multilocus sequence typing scheme for Enterococcus faecium. J Clin Microbiol 2002, 40:1963-1971.
10. Klare I., Konstabel C., Mueller-Bertling S., Werner G., Strommenger B., Kettlitz C., et al. Spread of ampicillin/vancomycin-resistant Enterococcus faecium of the epidemic-virulent clonal complex-17 carrying the genes esp and hyl in German hospitals. Eur J Clin Microbiol Infect Dis 2005, 24:815-825.
11. Lopez M., Saenz Y., Rojo-Bezares B., Martinez S., del Campo R., Ruiz-Larrea F., et al. Detection of vanA and vanB2-containing enterococci from food samples in Spain, including Enterococcus faecium strains of CC17 and the new singleton ST425. Int J Food Microbiol 2009, 133:172-178.
12. Pillai S.K., Sakoulas G., Gold H.S., Wennersten C., Eliopoulos G.M., Moellering R.C., et al. Prevalence of the fsr locus in Enterococcus faecalis infections. J Clin Microbiol 2002, 40:2651-2652.
13. Poeta P., Costa D., Saenz Y., Klibi N., Ruiz-Larrea F., Rodrigues J., et al. Characterization of antibiotic resistance genes and virulence factors in faecal enterococci of wild animals in Portugal. J Vet Med B Infect Dis Vet Public Health 2005, 52:396-402.
14. Poeta P., Costa D., Igrejas G., Rojo-Bezares B., Saenz Y., Zarazaga M., et al. Characterization of vanA-containing Enterococcus faecium isolates carrying Tn5397-like and Tn916/Tn1545-like transposons in wild boars (Sus scrofa). Microb Drug Resist 2007, 13:151-156.
15. Radhouani H., Poeta P., Pinto L., Miranda J., Coelho C., Carvalho C., et al. Proteomic characterization of vanA-containing Enterococcus recovered from Seagulls at the Berlengas Natural Reserve, W Portugal. Proteome Sci 2010, 8:48.
16. Sutcliffe J., Grebe T., Tait-Kamradt A., Wondrack L. Detection of erythromycin-resistant determinants by PCR. Antimicrob Agents Chemother 1996, 40:2562-2566.
17. Torres C., Tenorio C., Portillo A., Garcia M., Martinez C., Del Campo R., et al. Intestinal colonization by vanA- or vanB2-containing enterococcal isolates of healthy animals in Spain. Microb Drug Resist 2003, 9(Suppl. 1):S47-S52.
18. Woodford N., Adebiyi A.M., Palepou M.F., Cookson B.D. Diversity of VanA glycopeptide resistance elements in enterococci from humans and nonhuman sources. Antimicrob Agents Chemother 1998, 42:502-508.