Synthesis, cloning, and expression of Mycoplasma suis inorganic pyrophosphatase gene using PCR-based accurate synthesis and overlap-extension PCR, and its immunogenicity analysis

Research in Veterinary Science

Volumn 91, Issue 3, 2011, Pages

  Liu, Jianzhu ^a   Cheng, Ziqiang ^a   Zhou, Dong ^a   Zhang, Li ^a   Yan, Zhengui ^a   Wang, Zhenyong ^a   Yang, Dubao ^a   Liu, Yongxia ^a   Chai, Tongjie ^a  

^a SHANDONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Cloning; Expression; Mycoplasma suis; Ppa; Synthesizing

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE;

ANIMAL CELL; ANTIBODY RESPONSE; ARTICLE; BACTERIAL GENE; CELLULAR IMMUNITY; CODON; CONTROLLED STUDY; ENZYME ACTIVE SITE; ERYTHROCYTE; EXPRESSION VECTOR; GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENE EXPRESSION; GENE SYNTHESIS; HUMORAL IMMUNITY; IMMUNOGENICITY; MOLECULAR CLONING; MYCOPLASMA SUIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PPA GENE; PROTEIN DENATURATION; PROTEIN PURIFICATION; SEQUENCE HOMOLOGY;

CLONING, MOLECULAR; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; INORGANIC PYROPHOSPHATASE; MYCOPLASMA; POLYMERASE CHAIN REACTION;

MYCOPLASMA SUIS; PROKARYOTA; SUIDAE;

EID: 80555150855     PISSN: 00345288     EISSN: 15322661     Source Type: Journal    
DOI: 10.1016/j.rvsc.2011.02.009     Document Type: Article

References (12)

1. Congli Y., Zhibiao Y., Ningyu Z., Congbin Y., Xiaowe I.Y., Jianguo Z., Li C., Xiuguo H. The 18kb DNA fragment formerly confirmed as Mycoplasma suis M. suis specific was originated from the porcine genome. Veterinary Microbiology 2009, 138:197-198.
2. Hoelzle K., Doser S., Ritzmann M., Heinritzi K., Palzer A., Elicker S., Kramer M., Felder KM., Hoelzle L.E. Vaccination with the Mycoplasma suis recombinant adhesion protein MSG1 elicits a strong immune response but fails to induce protection in pigs. Vaccine 2009, 27:5376-5382.
3. Hoelzle L.E. Haemotrophic mycoplasmas: recent advances in Mycoplasma suis. Veterinary Microbiology 2008, 130:215-226.
4. Hoelzle L.E., Adelt D., Hoelzle K., Heinritzi K., Wittenbrink M.M. Development of a diagnostic PCR assay based on novel DNA sequences for the detection of Mycoplasma suis (Eperythrozoon suis) in porcine blood. Veterinary Microbiology 2003, 93:185-196.
5. Hoelzle L.E., Helbling M., Hoelzle K., Ritzmann M., Heinritzi K., Wittenbrink M.M. First LightCycler real-time PCR assay for the quantitative detection of Mycoplasma suis in clinical samples. Journal of Microbiological Methods 2007, 70:346-354.
6. Hoelzle L.E., Hoelzle K., Harder A., Ritzmann M., Aupperle H., Schoon H.A., Heinritzi K., Wittenbrink M.M. First identification and functional characterization of an immunogenic protein in unculturable haemotrophic Mycoplasmas (Mycoplasma suis HspA1). FEMS Immunology and Medical Microbiology 2007, 49:215-223.
7. Hoelzle L.E., Hoelzle K., Helbling M., Aupperle H., Schoon H.A., Ritzmann M., Heinritzi K., Felder K.M., Wittenbrink M.M. MSG1, a surface-localised protein of Mycoplasma suis is involved in the adhesion to erythrocytes. Microbes and Infection 2007, 9:466-474.
8. Hoelzle K., Peter S., Sidler M., Kramer M.M., Wittenbrink M.M., Felder K.M., Hoelzle L.E. Inorganic pyrophosphatase in uncultivable hemotrophic mycoplasmas: identification and properties of the enzyme from Mycoplasma suis. BMC Microbiology 2010, 10:194.
9. Ko K.M., Lee W., Yu J.R., Ahnn J. PYP-1, inorganic pyrophosphatase, is required for larval development and intestinal function in C. Elegans. FEBS letters 2007, 581:5445-5453.
10. Sambrook J., Russell D.W. Molecular Cloning: a Laboratory Manual 2001, Clod Spring Harbor Laboratory Press. third ed.
11. Usui Y., Uematsu T., Uchihashi T., Takahashi M., Takahashi M., Ishizuka M., Doto R., Tanaka H., Komazaki Y., Osawa M., Yamada K., Yamaoka M., Furusawa K. Inorganic polyphosphate induces osteoblastic differentiation. Journal of Dental Research. 2010, 89:504-509.
12. Xiong A.S., Yao Q.H., Peng R.H., Duan H., Li X., Fan H.Q., Cheng Z.M., Li Y. PCR-based accurate synthesis of long DNA sequences. Nature Protocol 2006, 1:791-797.