A laminar-flow chamber assay for measuring bacterial adhesion under shear stress

Methods in Molecular Biology

Volumn 799, Issue , 2012, Pages 185-195

  Soyer, Magali ^a,b   Duménil, Guillaume ^a,b  

^a PARIS CARDIOVASCULAR RESEARCH CENTER   (France)

^b UNIVERSITÉ PARIS DESCARTES   (France)

Author keywords

Bacterial adhesion; Bacterial proliferation; Microcirculation; Neisseria meningitidis; Septicemia; Shear stress

Indexed keywords

ARTICLE; BACTERIUM ADHERENCE; CULTURE TECHNIQUE; ENDOTHELIUM CELL; HUMAN; IN VITRO STUDY; METHODOLOGY; MICROBIOLOGY; MICROCIRCULATION; NEISSERIA MENINGITIDIS; PHYSIOLOGY; SHEAR STRENGTH; VIDEO MICROSCOPY;

BACTERIAL ADHESION; CELL CULTURE TECHNIQUES; ENDOTHELIAL CELLS; HUMANS; MICROCIRCULATION; MICROSCOPY, VIDEO; NEISSERIA MENINGITIDIS; SHEAR STRENGTH;

BACTERIA (MICROORGANISMS); NEISSERIA MENINGITIDIS;

EID: 80555127410     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-346-2_12     Document Type: Chapter

References (13)

1. McEver RP (2002) Selectins: lectins that initiate cell adhesion under flow. Curr Opin Cell Biol 14: 581–586.
2. Lehoux S, Tedgui A (2003) Cellular mechanics and gene expression in blood vessels. J Biomech 36: 631–643.
3. Janmey PA, Schliwa M (2008) Rheology. Curr Biol 18:R639–R641.
4. Valbuena G, Walker DH (2006) The endothelium as a target for infections. Annu Rev Pathol 1: 171–198.
5. Mairey E, Genovesio A, Donnadieu E et al (2006) Cerebral microcirculation shear stress levels determine Neisseria meningitidis attachment sites along the blood-brain barrier. J Exp Med 203: 1939–1950.
6. Mikaty G, Soyer M, Mairey E et al (2009) Extracellular bacterial pathogen induces host cell surface reorganization to resist shear stress. PLoS Pathog 5: e1000314.
7. Thomas WE, Trintchina E, Forero M et al (2002) Bacterial adhesion to target cells enhanced by shear force. Cell 109: 913–923.
8. Tchesnokova V, McVeigh AL, Kidd B et al (2010) Shear-enhanced binding of intestinal colonization factor antigen I of enterotoxigenic Escherichia coli. Mol Microbiol 76: 489–502.
9. Konto-Ghiorghi Y, Mairey E, Mallet A et al (2009) Dual role for pilus in adherence to epithelial cells and biofilm formation in Streptococcus agalactiae. PLoS Pathog 5: e1000422.
10. Kellogg DS, Jr. Cohen IR, Norins LC et al (1968) Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro. Journal of bacteriology 96: 596–605.
11. Soyer M, Duménil G (2011) Introducing shear stress in the study of bacterial adhesion. J Vis Exp. doi: 10.3791/3241. In press.
12. Abramoff MD, Magelhaes PJ, Ram SJ (2004) Image Processing with ImageJ. Biophotonics International 11: 36–42.
13. Solomon JM, Rupper A, Cardelli JA et al (2000) Intracellular growth of Legionella pneu-mophila in Dictyostelium discoideum, a system for genetic analysis of host-pathogen interactions. Infect and Immun 68: 2939–2947.