An evaluation of the performance and acceptability of three LED fluorescent microscopes in Zambia: Lessons learnt for scale-up

PLoS ONE

Volumn 6, Issue 11, 2011, Pages

  Turnbull, Eleanor R ^a,b   Kaunda, Kaunda ^a   Harris, Jennifer B ^a,b   Kapata, Nathan ^c   Muvwimi, Mweemba W ^c   Kruuner, Annika ^a,b   Henostroza, German ^a,b   Reid, Stewart E ^a,b  

^a CENTRE FOR INFECTIOUS DISEASE RESEARCH IN ZAMBIA   (Zambia)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^c MINISTRY OF HEALTH   (Zambia)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM CULTURE; CLINICAL EFFECTIVENESS; COMPARATIVE EFFECTIVENESS; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; HUMAN; INSTRUMENT VALIDATION; INTERMETHOD COMPARISON; INTERRATER RELIABILITY; LIGHT EMITTING DIODE; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SPUTUM SMEAR; TISSUE SECTION; ZAMBIA; EVALUATION; INSTRUMENTATION; LABORATORY DIAGNOSIS; STANDARD;

MICROSCOPY, FLUORESCENCE; SPECIMEN HANDLING; ZAMBIA;

EID: 80455149842     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0027125     Document Type: Article

References (33)

1. Government of the Republic of Zambia, Central Statistical Office, (2011) The Monthly, January 2011.
2. World Health Organization (2010) Global Tuberculosis Control: WHO Report 2010 Geneva, Switzerland. WHO/HTM/TB/2010.7.
3. Harris JB, Hatwiinda SM, Randels KM, Chi BH, Kancheya NG, et al. (2008) Early lessons from the integration of tuberculosis and HIV services in primary care centers in Lusaka, Zambia. Int J Tuberc Lung Dis 12: 773-779.
4. Mukadi YD, Maher D, Harries A, (2001) Tuberculosis case fatality rates in high HIV prevalence populations in sub-Saharan Africa. AIDS 15: 143-152.
5. Ba F, Rieder HL, (1999) A comparison of fluorescence microscopy with the Ziehl-Neelsen technique in the examination of sputum for acid-fast bacilli. Int J Tuberc Lung Dis 3: 1101-1105.
6. Mugusi F, Villamor E, Urassa W, Saathoff E, Bosch RJ, et al. (2006) HIV co-infection, CD4 cell counts and clinical correlates of bacillary density in pulmonary tuberculosis. Int J Tuberc Lung Dis 10: 663-669.
7. World Health Organization (2009): Global Tuberculosis Control- epidemiology, strategy, financing: WHO Report 2009. Geneva, Switzerland. WHO/HTM/TB/2009.411.
8. Steingart KR, Henry M, Ng V, Hopewell PC, Ramsay A, et al. (2006) Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review. Lancet Infect Dis 6: 570-581.
9. Cattamanchi A, Davis JL, Worodria W, den Boon S, Yoo S, et al. (2009) Sensitivity and specificity of fluorescence microscopy for diagnosing pulmonary tuberculosis in a high HIV prevalence setting. Int J Tuberc Lung Dis 13: 1130-1136.
10. Prasanthi K, Kumari AR, (2005) Efficacy of fluorochrome stain in the diagnosis of pulmonary tuberculosis co-infected with HIV. Indian J Med Microbiol 23: 179-181.
11. Toman K, (2004) What are the advantages and disadvantages of fluorescence microscopy? In: Frieden T, editors. Toman's tuberculosis: case detection, treatment, and monitoring-questions and answers. 2nd ed Geneva World Health Organization pp. 31-34.
12. World Health Organization (2010) Fluorescent Light Emitting Diode Microscopy for Diagnosis of Tuberculosis. Policy Statement. March 2010. http://www.stoptb.org/wg/gli/assets/documents/WHO%20Policy%20Statement%20on%20LED%20Microscopy%20FINAL%20March%202010.pdf Accessed 16 February 2011.
13. Trusov A, Bumgarner R, Valijev R, Chestnova R, Talevski S, et al. (2009) Comparison of Lumin LED fluorescent attachment, fluorescent microscopy and Ziehl-Neelsen for AFB diagnosis. Int J Tuberc Lung Dis 13: 836-841.
14. Marais BJ, Brittle W, Painczyk K, Hesseling AC, Beyers N, et al. (2008) Use of light-emitting diode fluorescence microscopy to detect acid-fast bacilli in sputum. Clin Infect Dis 47: 203-207.
15. Cattamanchi A, Huang L, Worodria W, den Boon S, Kalema N, et al. (2011) Integrated strategies to optimize sputum smear microscopy: a prospective observational study. Am J Respir Crit Care Med 183: 547-551.
16. Mizuno K, Chikamatsu K, Aono A, Azuma Y, Yamada H, et al. (2009) [Clinical evaluation of acid-fast smear examination with light emitting diode fluorescent microscopy]. Kekkaku 84: 627-629.
17. Hanscheid T, Ribeiro CM, Shapiro HM, Perlmutter NG, (2007) Fluorescence microscopy for tuberculosis diagnosis. Lancet Infect Dis 7: 236-237.
18. Minion J, Sohn H, Pai M, (2009) Light-emitting diode technologies for TB diagnosis: what is on the market? Expert Rev Med Devices 6: 341-345.
19. Van Deun A, Chonde TM, Gumusboga M, Rienthong S, (2008) Performance and acceptability of the FluoLED Easy module for tuberculosis fluorescence microscopy. Int J Tuberc Lung Dis 12: 1009-1014.
20. Hung NV, Sy DN, Anthony RM, Cobelens FG, van Soolingen D, (2007) Fluorescence microscopy for tuberculosis diagnosis. Lancet Infect Dis 7: 238-239; author reply 239-240.
21. Miller AR, Davis GL, Oden ZM, Razavi MR, Fateh A, et al. (2010) Portable, battery-operated, low-cost, bright field and fluorescence microscope. PLoS One 5.
22. Hanscheid T, (2008) The future looks bright: low-cost fluorescent microscopes for detection of Mycobacterium tuberculosis and Coccidiae. Trans R Soc Trop Med Hyg 102: 520-521.
23. Albert H, Manabe Y, Lukyamuzi G, Ademun P, Mukkada S, et al. (2010) Performance of three LED-based fluorescence microscopy systems for detection of tuberculosis in Uganda. PLoS One 5: e15206.
24. Bonnet M, Gagnidze L, Githui W, Guerin PJ, Bonte L, et al. (2011) Performance of LED-based fluorescence microscopy to diagnose tuberculosis in a peripheral health centre in Nairobi. PLoS One 6: e17214.
25. (2005) Government of the Republic of Zambia, Ministry of Health (2005), National Health Strategic Plan: 2006-2010. Ndeke House, Lusaka, Zambia.
26. Affolabi D, Torrea G, Odoun M, Senou N, Ali Ligali M, et al. (2010) Comparison of two LED fluorescence microscopy build-on modules for acid-fast smear microscopy. Int J Tuberc Lung Dis 14: 160-164.
27. World Health Organization (1998) Laboratory Services in Tuberculosis Control Part II Microscopy. WHO WHO/TB/98.258.
28. Siddiqi SH, R-G S, (2006) Foundation for Innovative New Diagnostics: MGIT Procedure Manual. Geneva, Switzerland.
29. Carl Zeiss Microimaging GmbH (2011) Primostar iLED, http://www.zeiss.de/c12567be0045acf1/Contents-Frame/8a410946841ce684c125746c006244ba. Accessed 26th May 2011.
30. LWScientific (2011) http://shop.lwscientific.com/ Accessed 26th May 2011.
31. Fraen Corporation (2011) http://www.fraensrl.com/flmicro.html. Accessed 26th May 2011.
32. Foundation for Innovative New Diagnostics (2008) Demonstration Project iLED Training Manual, Version 1.0, 1st September 2008.
33. Foundation for Innovative New Diagnostics (2010) FIND prices for Primostar iLED and Country List. http://www.finddiagnostics.org/programs/tb/find-negotiated-prices/primo-star-iled.html. Accessed 16th June 2011.