Performance of three LED-Based fluorescence microscopy systems for detection of tuberculosis in Uganda

PLoS ONE

Volumn 5, Issue 12, 2010, Pages

  Albert, Heidi ^a   Manabe, Yukari ^b   Lukyamuzi, George ^a   Ademun, Patrick ^a   Mukkada, Sheena ^a   Nyesiga, Barnabas ^a   Joloba, Moses ^c   Paramasivan, C N ^d   Perkins, Mark D ^d  

^a Foundation for Innovative New Diagnostics   (Uganda)

^b MULAGO HOSPITAL   (Uganda)

^c National Tuberculosis Reference Laboratory   (Uganda)

^d FOUNDATION FOR INNOVATIVE NEW DIAGNOSTICS   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM DETECTION; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; FLUORESCENCE MICROSCOPY; HUMAN; INTERMETHOD COMPARISON; LIGHT EMITTING DIODE; LUNG TUBERCULOSIS; MYCOBACTERIUM TUBERCULOSIS; QUALITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; SPUTUM EXAMINATION; ANALYTICAL EQUIPMENT; COMPARATIVE STUDY; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; INSTRUMENTATION; LABORATORY DIAGNOSIS; LIGHT; METABOLISM; METHODOLOGY; OBSERVER VARIATION; REPRODUCIBILITY; SPUTUM; UGANDA;

HUMAN IMMUNODEFICIENCY VIRUS; MYCOBACTERIUM TUBERCULOSIS;

FALSE POSITIVE REACTIONS; HIV INFECTIONS; HUMANS; LIGHT; MICROSCOPY, FLUORESCENCE; MYCOBACTERIUM TUBERCULOSIS; OBSERVER VARIATION; REAGENT KITS, DIAGNOSTIC; REPRODUCIBILITY OF RESULTS; SPUTUM; TUBERCULOSIS, PULMONARY; UGANDA;

EID: 78650826151     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0015206     Document Type: Article

References (25)

1. Steingart KR, Henry M, Ng V, Hopewell PC, Ramsay A, et al. (2006) Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review. Lancet Infect Dis 6(9): 570-581.
2. World Health Organisation (2008) Global tuberculosis control: surveillance, planning, financing: WHO Report 2008, Geneva, Switzerland. WHO/HTM/TB/2008.393.
3. Hagemann PKH (1937) Fluoreszenzmikroskopische untersuchungen uber virus und andere microben. Zentralbl Bakteriol 140: 184.
4. (2000) International Union Against Tuberculosis and Lung Disease (IUATLD) technical guide: sputum examination for tuberculosis by direct microscopy in low-income countries.5th Ed. Paris: IUATLD.
5. Cambanis A, Ramsay A, Wirkom V, Tata E, Cuevas LE (2007) Investing time in microscopy: an opportunity to optimize smear-based case detection of tuberculosis. Int J Tuberc Lung Dis 11: 40-45.
6. Anthony RM, Kolk AH, Kuijper S, Klatser PR (2006) Light-emitting diodes for auramine O fluorescence microscopic screening of Mycobacterium tuberculosis. Int J Tuberc Lung Dis 0(9): 1060-1062.
7. Marais BJ, Brittle W, Painczyk K, Hesseling AC, Beyers N, et al. (2008) Use of light-emitting diode fluorescence microscopy to detect acid-fast bacilli in sputum. Clin Infect Dis 47(2): 203-7.
8. Minion J, Sohn H, Pai M (2009) Light-emitting diode technologies for TB diagnosis: what is on the market? Expert Rev Med Devices 6(4): 341-345.
9. Nabeta P, Ha DT, Michael JS, Hofmann H, Krapp F, et al. (2009) LED-based fluorescence microscope for TB detection: evaluation in reference laboratories. Int J Tuberc Lung Dis 2009 (Suppl): S123.
10. Van Deun A, Chonde TM, Gumusboga M, Rienthong S (2009) Performance and acceptability of the FluoLED Easy module for tuberculosis fluorescence microscopy. Int J Tuberc Lung Dis 12(9): 1009-1014.
11. Boehme C, Muniyandi M, Michael JS, Hanif M, Katoch VM, et al. (2009) Implementation of LED-based fluorescence microscope in microscopy centres in India. Int J Tuberc Lung Dis. S124.
12. World Health Organisation (2010) Fluorescent Light Emitting Diode Microscopy for Diagnosis of Tuberculosis. Policy Statement. March 2010. http://www.stoptb.org/wg/gli/assets/documents/WHOPolicyStatementonLEDMicroscopyFINALMarch2010.pdf Accessed 28 July 2010.
13. Carl Zeiss Microimaging GmbH (2009) Primostar iLED. http://www.zeiss.de/c12567be0045acf1/Contents-Frame/8a410946841ce684c125746c006244ba Accessed 30 July 2009.
14. LWScientific http://shop.lwscientific.com/s.nl/it.A/id.1746/.f?sc=9&category=3603 Accessed 30 July 2009.
15. Fraen Corporation (2009) http://www.fraensrl.com/flmicro.html Accessed 30 July 2009.
16. Foundation for Innovative New Diagnostics (2006) MGIT Procedure Manual. Geneva, Switzerland.
17. Tauns Laboratories, Inc. (2010) Capilia TB-Neo. Product insert. http://www.tauns.co.jp/english/product/Capiliatb-Neo_insert.pdf. Accessed 4 October 2010.
18. World Health Organisation (1998) WHO Laboratory Services in Tuberculosis Control. Part II: Microscopy. WHO/TB/98.258. Geneva, Switzerland.
19. Revised National Tuberculosis Control Programme Laboratory Network (2005) Guidelines for Quality Assurance of Smear Microscopy for Diagnosing Tuberculosis.April 2005. New Delhi: Ministry of Health and Family Welfare.
20. Bennedsen J, Larsen SO (1966) Examination for tubercle bacilli by fluorescence microscopy. Scand J Respir Dis 47: 114-120.
21. Affolabi D, Torrea G, Odoun M, Senou N, Ali Ligali M, et al. (2010) Comparison of two LED fluorescence microscopy build-in modules for acid-fast smear microscopy. Int J Tuberc Lung Dis 14(2): 160-164.
22. Miller AR, Davis GL, Oden ZM, Razavi MR, Fateh A, et al. (2010) Portable, Battery-Operated, Low-Cost, Bright Field and Fluorescence Microscope. PLoS ONE: 5(8): e1189990. doi:10.1371/journal.pone.0011890.
23. Breslauer DN, Maamari RN, Switz NA, Lam WA, Fletcher DA (2009) Mobile Phone Based Clinical Microscopy for Global Health Applications. PLoS ONE: 4(7): e6320. doi:10.1371/journal.pone.0006320.
24. Foundation for Innovative New Diagnostics (2010) FIND prices for Primostar iLED and Country List. http://www.finddiagnostics.org/programs/find-negotiated-prices/primo-star-iled.html Accessed 28 October 2010.
25. Bonnet M, Gagnidze L, Githui W, Osana LA, Chege AM, et al. (2009) Concentrated sputum and light emitting diode fluorescence microscopy to diagnose tuberculosis. Int J Tuberc Lung Dis. S117.