Arabidopsis thaliana polar glycerolipid profiling by thin layer chromatography (TLC) coupled with gas-liquid chromatography (GLC)

Journal of Visualized Experiments

Volumn , Issue 49, 2011, Pages

  Wang, Zhen ^a   Benning, Christoph ^a  

^a Michigan State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355149176     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2518     Document Type: Article

References (20)

1. I. Ajjawi, et al., Large-scale reverse genetics in Arabidopsis: case studies from the Chloroplast 2010 Project, Plant Physiol 152(2), 529 (2010).
2. M. X. Andersson, J. M. Kjellberg, and A. S. Sandelius, The involvement of cytosolic lipases in converting phosphatidyl choline to substrate for galactolipid synthesis in the chloroplast envelope, Biochim. Biophys. Acta 1684(1-3), 46 (2004).
3. K. Awai, et al., A phosphatidic acid-binding protein of the chloroplast inner envelope membrane involved in lipid trafficking, Proc. Natl. Acad. Sci. U. S. A 103(28), 10817 (2006).
4. C. Benning, Mechanisms of lipid transport involved in organelle biogenesis in plant cells, Annu. Rev. Cell Dev. Biol. 25, 71 (2009).
5. J. Browse, P. J. McCourt, and C. R. Somerville, Fatty acid composition of leaf lipids determined after combined digestion and fatty acid methyl ester formation from fresh tissue, Anal. Biochem. 152(1), 141 (1986).
6. P. Dormann, I. Balbo, and C. Benning, Arabidopsis galactolipid biosynthesis and lipid trafficking mediated by DGD1, Science 284(5423), 2181 (1999).
7. N. Gaude, et al., Phospholipase C5 (NPC5) is involved in galactolipid accumulation during phosphate limitation in leaves of Arabidopsis, Plant J.
8. A. Guskov, et al., Cyanobacterial photosystem II at 2.9-A resolution and the role of quinones, lipids, channels and chloride, Nat. Struct. Mol. Biol. 16(3), 334 (2009).
9. H. Hartel, P. Dormann, and C. Benning, DGD1-independent biosynthesis of extraplastidic galactolipids after phosphate deprivation in Arabidopsis, Proc. Natl. Acad. Sci. U. S. A 97(19), 10649 (2000).
10. A. T. James and A. J. Martin, Gas-liquid partition chromatography; the separation and micro-estimation of volatile fatty acids from formic acid to dodecanoic acid, Biochem. J. 50(5), 679 (1952).
11. P. Jordan, et al., Three-dimensional structure of cyanobacterial photosystem I at 2.5 A resolution, Nature 411(6840), 909 (2001).
12. K. Kobayashi, et al., Type-B monogalactosyldiacylglycerol synthases are involved in phosphate starvation-induced lipid remodeling, and are crucial for low-phosphate adaptation, Plant J. 57(2), 322 (2009).
13. B. Lu, et al., A small ATPase protein of Arabidopsis, TGD3, involved in chloroplast lipid import, J. Biol. Chem. 282(49), 35945 (2007).
14. J. Ohlrogge and J. Browse, Lipid biosynthesis, Plant Cell 7(7), 957 (1995).
15. E. Stahl, [Thin-layer chromatography; methods, influencing factors and an example of its use.], Pharmazie 11(10), 633 (1956).
16. W. Stoffel, W. INSULL, Jr., and E. H. Ahrens, Jr., Gas-liquid chromatography of highly unsaturated fatty acid methyl esters, Proc. Soc. Exp. Biol. Med. 99(1), 238 (1958).
17. R. Welti, X. Wang, and T. D. Williams, Electrospray ionization tandem mass spectrometry scan modes for plant chloroplast lipids, Anal. Biochem. 314(1), 149 (2003).
18. C. Xu, et al., Lipid trafficking between the endoplasmic reticulum and the plastid in Arabidopsis requires the extraplastidic TGD4 protein, Plant Cell 20(8), 2190 (2008).
19. C. Xu, et al., Mutation of the TGD1 chloroplast envelope protein affects phosphatidate metabolism in Arabidopsis, Plant Cell 17(11), 3094 (2005).
20. C. Xu, et al., A permease-like protein involved in ER to thylakoid lipid transfer in Arabidopsis, EMBO J. 22(10), 2370 (2003).