Arabidopsis galactolipid biosynthesis and lipid trafficking mediated-by DGD1

Science

Volumn 284, Issue 5423, 1999, Pages 2181-2184

  Dörmann, Peter ^a   Balbo, Ilse ^b   Benning, Christoph ^a  

^a Michigan State University   (United States)

^b MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; DIACYLGLYCEROL DERIVATIVE; GALACTOLIPID; MEMBRANE LIPID; UNCLASSIFIED DRUG;

ARABIDOPSIS; ARTICLE; CHLOROPLAST; DNA DETERMINATION; GENE ISOLATION; LIPID TRANSPORT; LIPOGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOTOSYNTHESIS; PRIORITY JOURNAL; THYLAKOID MEMBRANE;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; BASE SEQUENCE; CHLOROPLASTS; CHROMOSOME MAPPING; DNA, COMPLEMENTARY; ENDOPLASMIC RETICULUM; EXONS; GALACTOLIPIDS; GALACTOSYLTRANSFERASES; GENES, PLANT; GLYCOLIPIDS; INTRACELLULAR MEMBRANES; LIPID METABOLISM; MOLECULAR SEQUENCE DATA; MUTATION; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 0242504168     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5423.2181     Document Type: Article

References (34)

1. J. Browse and C. Somerville, Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 467 (1991); J. Joyard et al., Plant Physiol. 118, 715 (1998).
2. J. Browse and C. Somerville, Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 467 (1991); J. Joyard et al., Plant Physiol. 118, 715 (1998).
3. L. Ohlsson, M. Blom, K. Bohlinder, A. Carlsson, A. Nilsson, J. Nutr. 128, 239 (1998); L. Andersson et al., J. Lipid Res. 36, 1392 (1995).
4. L. Ohlsson, M. Blom, K. Bohlinder, A. Carlsson, A. Nilsson, J. Nutr. 128, 239 (1998); L. Andersson et al., J. Lipid Res. 36, 1392 (1995).
5. E. Heinz and P. G. Roughan, Plant Physiol. 72, 273 (1983).
6. S. Mongrand, J.-J. Bessoule, F. Cabantous, C. Cassagne, Phytochemistry 49, 1049 (1998).
7. L. Kunst, J. Browse, C. R. Somerville, Proc. Natl. Acad. Sci. U.S.A. 85, 4143 (1988).
8. J. Browse and C. R. Somerville, in Arabidopsis, E. M. Meyerowitz and C. R. Somerville, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1994), pp. 881-912.
9. P. Dörmann, S. Hoffmann-Benning, I. Balbo, C. Benning, Plant Cell 7, 1801 (1995).
10. H. Härtel, H. Lokstein, P. Dörmann, B. Grimm, C. Benning, Plant Physiol. 115, 1175 (1997); F. Reifarth et al., Biochemistry 36, 11769 (1997); H. Härtel, H. Lokstein, P. Dörmann, R. N. Trethewey, C. Benning, Plant Physiol. Biochem. 36, 407 (1998); L.-J. Chen and H.-M. Li, Plant J. 16, 33 (1998).
11. H. Härtel, H. Lokstein, P. Dörmann, B. Grimm, C. Benning, Plant Physiol. 115, 1175 (1997); F. Reifarth et al., Biochemistry 36, 11769 (1997); H. Härtel, H. Lokstein, P. Dörmann, R. N. Trethewey, C. Benning, Plant Physiol. Biochem. 36, 407 (1998); L.-J. Chen and H.-M. Li, Plant J. 16, 33 (1998).
12. H. Härtel, H. Lokstein, P. Dörmann, B. Grimm, C. Benning, Plant Physiol. 115, 1175 (1997); F. Reifarth et al., Biochemistry 36, 11769 (1997); H. Härtel, H. Lokstein, P. Dörmann, R. N. Trethewey, C. Benning, Plant Physiol. Biochem. 36, 407 (1998); L.-J. Chen and H.-M. Li, Plant J. 16, 33 (1998).
13. H. Härtel, H. Lokstein, P. Dörmann, B. Grimm, C. Benning, Plant Physiol. 115, 1175 (1997); F. Reifarth et al., Biochemistry 36, 11769 (1997); H. Härtel, H. Lokstein, P. Dörmann, R. N. Trethewey, C. Benning, Plant Physiol. Biochem. 36, 407 (1998); L.-J. Chen and H.-M. Li, Plant J. 16, 33 (1998).
14. A. van Besouw and J. F. G. M. Wintermans, Biochim. Biophys. Acta 529, 44 (1978); J. W. M. Heemskerk, T. Storz, R. R. Schmidt, E. Heinz, Plant Physiol. 93, 1286 (1990).
15. A. van Besouw and J. F. G. M. Wintermans, Biochim. Biophys. Acta 529, 44 (1978); J. W. M. Heemskerk, T. Storz, R. R. Schmidt, E. Heinz, Plant Physiol. 93, 1286 (1990).
16. J. Browse, N. Warwick, C. R. Somerville, C. R. Slack, Biochem. J. 235, 25 (1986).
17. 2 plants were homozygous act1,dgd1 double mutants with reduced amounts of 7,10,13-hexadecatrienoic acid as in act1 and reduced amounts of the digalactosyl lipid as in dgd1.
18. M. Shimojima et al., Proc. Natl. Acad. Sci. U.S.A. 94, 333 (1997).
19. M. A. Block, A.-J. Dorne, J. Joyard, R. Douce, J. Biol. Chem. 258, 13281 (1983).
20. K. Cline and K. Keegstra, Plant Physiol. 71, 366 (1983).
21. T. Teucher and E. Heinz, Planta 184, 319 (1991); E. Maréchal, M. A. Block, J. Joyard, R. Douce, J. Biol. Chem. 269, 5788 (1994).
22. T. Teucher and E. Heinz, Planta 184, 319 (1991); E. Maréchal, M. A. Block, J. Joyard, R. Douce, J. Biol. Chem. 269, 5788 (1994).
23. 2 plants from the cross dgd1 × Ler for crossovers between markers nga127 and ATHCHIB. We used this mapping population to score markers g2488a and 31A-H. We obtained the RFLP markers from the Arabidopsis Biological Resource Center (g4523, fad7, g2488a, g4547) or from genomic fragments (31A-H, 5E-5, 18A-1) isolated from cosmids.
24. We used DNA of fad7, g4547, and g2488a to isolate genomic DNA fragments from different libraries (CIC yeast artificial chromosome library [C. Camilleri et al., Plant J. 14, 633 (1998)], IGF bacterial artificial chromosome library [T. Mozo, S. Fischer, H. Shizuya, T. Altmann, Mol. Gen. Genet. 258, 562 (1998)], and Arabidopsis cosmid library [K. Meyer, G. Benning, E. Grill, in Genome Mapping in Plants, A. H. Patterns, Ed. (Academic Press, New York, 1996), pp. 137-154]).
25. We used DNA of fad7, g4547, and g2488a to isolate genomic DNA fragments from different libraries (CIC yeast artificial chromosome library [C. Camilleri et al., Plant J. 14, 633 (1998)], IGF bacterial artificial chromosome library [T. Mozo, S. Fischer, H. Shizuya, T. Altmann, Mol. Gen. Genet. 258, 562 (1998)], and Arabidopsis cosmid library [K. Meyer, G. Benning, E. Grill, in Genome Mapping in Plants, A. H. Patterns, Ed. (Academic Press, New York, 1996), pp. 137-154]).
26. We used DNA of fad7, g4547, and g2488a to isolate genomic DNA fragments from different libraries (CIC yeast artificial chromosome library [C. Camilleri et al., Plant J. 14, 633 (1998)], IGF bacterial artificial chromosome library [T. Mozo, S. Fischer, H. Shizuya, T. Altmann, Mol. Gen. Genet. 258, 562 (1998)], and Arabidopsis cosmid library [K. Meyer, G. Benning, E. Grill, in Genome Mapping in Plants, A. H. Patterns, Ed. (Academic Press, New York, 1996), pp. 137-154]).
27. 2 plants (one to three independent crosses per cosmid) carrying the T-DNA, all were phenotypically wild type.
28. 2 plants (one to three independent crosses per cosmid) carrying the T-DNA, all were phenotypically wild type.
29. U. Uwer, L. Willmitzer, T. Altmann, Plant Cell 10, 1277 (1998).
30. For direct complementation analysis, the DGD1 cDNA released from pBluescriptllSK(+) with Sma I, Xho I was ligated into Sma I, Sal I of pBINAR-Hyg [D. Becker, Nucleic Acids Res. 18, 203 (1990); A. von Schaeven, thesis, Freie Universität Berlin (1989)] in the sense orientation behind the CaMV 35S promoter. This construct was directly transferred into dgd1.
31. For direct complementation analysis, the DGD1 cDNA released from pBluescriptllSK(+) with Sma I, Xho I was ligated into Sma I, Sal I of pBINAR-Hyg [D. Becker, Nucleic Acids Res. 18, 203 (1990); A. von Schaeven, thesis, Freie Universität Berlin (1989)] in the sense orientation behind the CaMV 35S promoter. This construct was directly transferred into dgd1.
32. A 459-bp Xho I, Pvu II fragment including the expression cassette was isolated from pQE31 (Qiagen Inc.) and ligated into the Sal I, Eco RV sites of pACYC184 [A. C. Y. Chang and S. N. Cohen, J. Bacteriol. 134, 1141 (1978)], giving rise to the plasmid pACYC-31. We amplified the open reading frame of the DGD1 cDNA by polymerase chain reaction with the primers (5′-GCG-GATCCGGTAAAGGAAACTCTAATT) and (5′-TTCTG-CAGTCTACCAGCCGAAGATTGG), thereby introducing a Bam HI site at the 5′ terminus and a Pst I site at the 3′ terminus. We ligated this cDNA fragment (Bam HI/Pst I) into pACYC-31. The resulting plasmid pACYC-31/239 was transferred into XL1-Blue cells containing the expression vector pGEX-3X with the cucumber monogalactosyl lipid synthase cDNA (12).
33. A.-J. Dorne, M. A. Block, J. Joyard, R. Douce, FEBS Lett. 145, 30 (1982).
34. Supported in part by grants from the US Department of Energy and from the Bundesministerium für Bildung und Forschung to C.B. and by a Feodor Lynen fellowship from the Alexander von Humboldt-Foundation and a research scholarship from the Deutsche Forschungsgemeinschaft to P.D. We are indebted to Ed Tolbert for stimulating discussions up until his death in December 1998. We thank H. Ohta for providing the monogalactosyl lipid synthase plasmid. We are grateful to B. Hintzmann and A. Rosenthal for sequencing genomic DNA and cDNA clones. The help of J. Huber in screening and analyzing complemented plants is especially acknowledged.