Non-surgical intratracheal instillation of mice with analysis of lungs and lung draining lymph nodes by flow cytometry

Journal of Visualized Experiments

Volumn , Issue 51, 2011, Pages

  Rayamajhi, Manira ^a   Redente, Elizabeth F ^b   Condon, Tracy V ^a   Gonzalez Juarrero, Mercedes ^c   Riches, David W H ^a,b   Lenz, Laurel L ^a,b  

^a UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

^b DEPARTMENT OF MEDICINE   (United States)

^c Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

Flow cytometry; Immunology; Intratracheal; Issue 51; Lung draining lymph nodes; Lungs; Mouse

Indexed keywords

EID: 80355149021     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2702     Document Type: Article

References (14)

1. Grayson, M. H. et al., Controls for lung dendritic cell maturation and migration during respiratory viral infection. J Immunol 179 (3), 1438 (2007).
2. GeurtsvanKessel, C. H. and Lambrecht, B. N., Division of labor between dendritic cell subsets of the lung. Mucosal Immunol 1 (6), 442 (2008).
3. Bar-Haim, E. et al., Interrelationship between dendritic cell trafficking and Francisella tularensis dissemination following airway infection. PLoS Pathog 4 (11), e1000211 (2008).
4. Kim, T. S. and Braciale, T. J., Respiratory dendritic cell subsets differ in their capacity to support the induction of virus-specific cytotoxic CD8+ T cell responses. PLoS One 4 (1), e4204 (2009).
5. Bakocevic, N., Worbs, T., Davalos-Misslitz, A., and Forster, R., T cell-dendritic cell interaction dynamics during the induction of respiratory tolerance and immunity. J Immunol 184 (3), 1317 (2010).
6. Thomas, R. J. et al., Influence of particle size on the pathology and efficacy of vaccination in a murine model of inhalational anthrax. J Med Microbiol (2010).
7. Kiyono, H. and Fukuyama, S., NALT- versus Peyer's-patch-mediated mucosal immunity. Nat Rev Immunol 4 (9), 699 (2004).
8. Parungo, C. P. et al., Lymphatic drainage of the peritoneal space: a pattern dependent on bowel lymphatics. Ann Surg Oncol 14 (2), 286 (2007).
9. Jakubzick, C., Helft, J., Kaplan, T. J., and Randolph, G. J., Optimization of methods to study pulmonary dendritic cell migration reveals distinct capacities of DC subsets to acquire soluble versus particulate antigen. J Immunol Methods 337 (2), 121 (2008).
10. Higgins, D. M. et al., Relative levels of M-CSF and GM-CSF influence the specific generation of macrophage populations during infection with Mycobacterium tuberculosis. J Immunol 180 (7), 4892 (2008).
11. Kool, M. et al., Alum adjuvant boosts adaptive immunity by inducing uric acid and activating inflammatory dendritic cells. J Exp Med 205 (4), 869 (2008).
12. Costa, D. L., Lehmann, J. R., Harold, W. M., and Drew, R. T., Transoral tracheal intubation of rodents using a fiberoptic laryngoscope. Lab Anim Sci 36 (3), 256 (1986).
13. Takahashi, S. and Patrick, G., Patterns of lymphatic drainage to individual thoracic and cervical lymph nodes in the rat. Lab Anim 21 (1), 31 (1987).
14. Van den Broeck, W., Derore, A., and Simoens, P., Anatomy and nomenclature of murine lymph nodes: Descriptive study and nomenclatory standardization in BALB/cAnNCrl mice. J Immunol Methods 312 (1-2), 12 (2006).