Preparation of drosophila polytene chromosome squashes for antibody labeling

Journal of Visualized Experiments

Volumn , Issue 36, 2010, Pages

  Cai, Weili ^a   Jin, Ye ^a   Girton, Jack ^a   Johansen, Jorgen ^a   Johansen, Kristen M ^a  

^a IOWA STATE UNIVERSITY   (United States)

Author keywords

Antibody labeling; Cellular Biology; Chromosomes; Drosophila; Issue 36; Polytene squash preparations

Indexed keywords

EID: 80355147706     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1748     Document Type: Article

References (3)

1. Cai, W., Bao, X., Deng, H., Jin, Y., Girton, J., Johansen, J. & Johansen, K.M. RNA polymerase II-mediated transcription at active loci does not require histone H3S10 phosphorylation in Drosophila. Development 135, 2917-2925, (2008).
2. DiMario, P., Rosby, R., & Cui, Z. Direct visualization of GFP-fusion proteins on polytene chromosomes. Dros. Inf. Serv. 89, 115-118, (2006).
3. Johansen, K.M., Cai, W., Deng, H., Bao, X, Zhang, W., Girton, J. & Johansen, J. Methods for studying transcription and epigenetic chromatin modification in Drosophila polytene chromosome squash preparations using antibodies. Methods 48, 387-397, (2009).