Visualizing dengue virus through Alexa Fluor labeling

Journal of Visualized Experiments

Volumn , Issue 53, 2011, Pages

  Zhang, Summer ^a   Tan, Cheng ^b   Ooi, Eong ^b  

^a DSO NATIONAL LABORATORIES   (Singapore)

^b DUKE NUS MEDICAL SCHOOL   (Singapore)

Author keywords

Alexa Fluor; Dengue virus; Fluorescence; Immunology; Issue 53; Labeling

Indexed keywords

EID: 80355145765     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3168     Document Type: Article

References (10)

1. Olenych, S. G., Claxton, N. S., Ottenberg, G. K. & Davidson, M. W. The Fluorescent Protein Color Palette. Vol. 21.5 1-34 (Current Protocols in Cell Biology, 2007).
2. Panchuk-Voloshina, N. et al. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J Histochem Cytochem 47, 1179-1188 (1999).
3. Shaner, N. C., Patterson, G. H. & Davidson, M. W. Advances in fluorescent protein technology. J Cell Sci 120, 4247-4260, doi:120/24/4247 [pii] 10.1242/jcs.005801 (2007).
4. Lippincott-Schwartz, J. & Patterson, G. H. Development and use of fluorescent protein markers in living cells. Science 300, 87-91, doi:10.1126/science.1082520 300/5616/87 [pii] (2003).
5. Kuhn, R. J. et al. Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. Cell 108, 717-725, doi:S0092867402006608 [pii] (2002).
6. Aguilar, J. S., Roy, D., Ghazal, P. & Wagner, E. K. Dimethyl sulfoxide blocks herpes simplex virus-1 productive infection in vitro acting at different stages with positive cooperativity. Application of micro-array analysis. BMC Infect Dis 2, 9 (2002).
7. Zhang, S. L., Tan, H. C., Hanson, B. J. & Ooi, E. E. A simple method for Alexa Fluor dye labelling of dengue virus. J Virol Methods 167, 172-177, doi:S0166-0934(10)00130-8 [pii] 10.1016/j.jviromet.2010.04.001 (2010).
8. Alexa Fluor Succinimidyl Esters (Molecular Probes), (2009).
9. Huang, S. et al. Analysis of proteins stained by Alexa dyes. Electrophoresis 25, 779-784, doi:10.1002/elps.200305723 (2004).
10. Freistadt, M. S. & Eberle, K. E. Fluorescent poliovirus for flow cytometric cell surface binding studies. J Virol Methods 134, 1-7, doi:S0166-0934(05)00272-7 [pii] 10.1016/j.jviromet.2005.08.011 (2006).