A simple method for Alexa Fluor dye labelling of dengue virus

Journal of Virological Methods

Volumn 167, Issue 2, 2010, Pages 172-177

  Zhang, Summer Li Xin ^a,b   Tan, Hwee Cheng ^b   Hanson, Brendon J ^a   Ooi, Eng Eong ^a,b  

^a DSO NATIONAL LABORATORIES   (Singapore)

^b DUKE NUS MEDICAL SCHOOL   (Singapore)

Author keywords

Alexa Fluor; Dengue virus; Fluorescence; Fluorescent virus; Labelling

Indexed keywords

ALEXA FLUOR SUCCINIMIDYL ESTER DYE; BICARBONATE; DYE; FLUORESCEIN; RHODAMINE; UNCLASSIFIED DRUG; VIRUS ANTIBODY;

ANIMAL CELL; ANTIBODY SPECIFICITY; ARTICLE; CHEMICAL LABELING; CONTROLLED STUDY; DENGUE VIRUS; DISSOLUTION; ENDOSOME; INTRACELLULAR TRANSPORT; NONHUMAN; PH; PRIORITY JOURNAL; VIROLOGY; VIRUS CELL INTERACTION;

ANIMALS; CELL LINE; CERCOPITHECUS AETHIOPS; CRICETINAE; DENGUE VIRUS; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; LIGHT; MICROBIAL VIABILITY; ORGANIC CHEMICALS; STAINING AND LABELING; VIROLOGY;

ALEXA; DENGUE VIRUS;

EID: 77953538590     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.04.001     Document Type: Article

References (12)

1. Aguilar J.S., Roy D., Ghazal P., Wagner E.K. Dimethyl sulfoxide blocks herpes simplex virus-1 productive infection in vitro acting at different stages with positive cooperativity. Application of micro-array analysis. BMC Infect. Dis. 2002, 2:9.
2. Freistadt M.S., Eberle K.E. Fluorescent poliovirus for flow cytometric cell surface binding studies. J. Virol. Methods 2006, 134:1-7.
3. Gubler D.J. Dengue and dengue hemorrhagic fever. Clin. Microbiol. Rev. 1998, 11:480-496.
4. Huang S., Wang H., Carroll C.A., Hayes S.J., Weintraub S.T., Serwer P. Analysis of proteins stained by Alexa dyes. Electrophoresis 2004, 25:779-784.
5. Kuhn R.J., Zhang W., Rossmann M.G., Pletnev S.V., Corver J., Lenches E., Jones C.T., Mukhopadhyay S., Chipman P.R., Strauss E.G., Baker T.S., Strauss J.H. Structure of dengue virus: implications for flavivirus organization, maturation, and fusion. Cell 2002, 108:717-725.
6. Lai Y.L., Chung Y.K., Tan H.C., Yap H.F., Yap G., Ooi E.E., Ng L.C. Cost-effective real-time reverse transcriptase PCR (RT-PCR) to screen for Dengue virus followed by rapid single-tube multiplex RT-PCR for serotyping of the virus. J. Clin. Microbiol. 2007, 45:935-941.
7. Martin N.C., Pardo J., Simmons M., Tjaden J.A., Widjaja S., Marovich M.A., Sun W., Porter K.R., Burgess T.H. An immunocytometric assay based on dengue infection via DC-SIGN permits rapid measurement of anti-dengue neutralizing antibodies. J. Virol. Methods 2006, 134:74-85.
8. Tassaneetrithep B., Burgess T.H., Granelli-Piperno A., Trumpfheller C., Finke J., Sun W., Eller M.A., Pattanapanyasat K., Sarasombath S., Birx D.L., Steinman R.M., Schlesinger S., Marovich M.A. DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells. J. Exp. Med. 2003, 197:823-829.
9. van der Schaar H.M., Rust M.J., Chen C., van der Ende-Metselaar H., Wilschut J., Zhuang X., Smit J.M. Dissecting the cell entry pathway of dengue virus by single-particle tracking in living cells. PLoS Pathog. 2008, 4:e1000244.
10. van der Schaar H.M., Rust M.J., Waarts B.L., van der Ende-Metselaar H., Kuhn R.J., Wilschut J., Zhuang X., Smit J.M. Characterization of the early events in dengue virus cell entry by biochemical assays and single-virus tracking. J. Virol. 2007, 81:12019-12028.
11. WHO, 2007. Report of Dengue Scientific Working Group. TDR/SWG/08, Geneva.
12. Wu L., Martin T.D., Carrington M., KewalRamani V.N. Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission. Virology 2004, 318:17-23.