Quantification of proteins using Peptide immunoaffinity enrichment coupled with mass spectrometry

Journal of Visualized Experiments

Volumn , Issue 53, 2011, Pages

  Zhao, Lei ^a   Whiteaker, Jeffrey R ^a   Pope, Matthew E ^b   Kuhn, Eric ^c   Jackson, Angela ^d   Anderson Leigh, N ^e   Pearson, Terry W ^b   Carr, Steven A ^c   Paulovich, Amanda G ^a  

^a Fred Hutchinson Cancer Research Center   (United States)

^b UNIVERSITY OF VICTORIA   (Canada)

^c BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^d UNIVERSITY OF VICTORIA   (Canada)

^e The Plasma Proteome Institute   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355145686     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2812     Document Type: Article

References (16)

1. Hoofnagle, A.N. & Wener, M.H. The fundamental flaws of immunoassays and potential solutions using tandem mass spectrometry. J. Immunol. Methods 347, 3-11 (2009).
2. Anderson, N.L., et al. Mass spectrometric quantitation of peptides and proteins using Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA). J Proteome Res 3, 235-44 (2004).
3. Chace, D.H. & Kalas, T.A. A biochemical perspective on the use of tandem mass spectrometry for newborn screening and clinical testing. Clin Biochem 38, 296-309 (2005).
4. Want, E.J., Cravatt, B.F. & Siuzdak, G. The expanding role of mass spectrometry in metabolite profiling and characterization. Chembiochem 6, 1941-51 (2005).
5. Barr, J.R., et al. Isotope dilution--mass spectrometric quantification of specific proteins: model application with apolipoprotein A-I. Clin Chem 42, 1676-82 (1996).
6. Gerber, S.A., Rush, J., Stemman, O., Kirschner, M.W. & Gygi, S.P. Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS. Proc Natl Acad Sci U S A 100, 6940-5 (2003).
7. Kuhn, E., et al. Quantification of C-reactive protein in the serum of patients with rheumatoid arthritis using multiple reaction monitoring mass spectrometry and 13C-labeled peptide standards. Proteomics 4, 1175-86 (2004).
8. Whiteaker, J.R., et al. Antibody-based enrichment of peptides on magnetic beads for mass-spectrometry-based quantification of serum biomarkers. Anal Biochem 362, 44-54 (2007).
9. Hoofnagle, A.N., Becker, J.O., Wener, M.H. & Heinecke, J.W. Quantification of thyroglobulin, a low-abundance serum protein, by immunoaffinity peptide enrichment and tandem mass spectrometry. Clin Chem 54, 1796-804 (2008).
10. Kuhn, E., et al. Developing Multiplexed Assays for Troponin I and Interleukin-33 in Plasma by Peptide Immunoaffinity Enrichment and Targeted Mass Spectrometry. Clin Chem. 55, 1108-1117 (2009).
11. Whiteaker, J.R., Zhao, L., Anderson, L. & Paulovich, A.G. An automated and multiplexed method for high throughput peptide immunoaffinity enrichment and multiple reaction monitoring mass spectrometry-based quantification of protein biomarkers. Mol. Cell. Proteomics 9, 184-196 (2010).
12. Neubert, H., Gale, J. & Muirhead, D. Online high-flow peptide immunoaffinity enrichment and nanoflow LC-MS/MS: assay development for total salivary pepsin/pepsinogen. Clin. Chem. 56, 1413-1423 (2010).
13. Ackermann, B.L. & Berna, M.J. Coupling immunoaffinity techniques with MS for quantitative analysis of low-abundance protein biomarkers. Expert Rev. Proteomics 4, 175-186 (2007).
14. MacLean, B., et al. Skyline: an open source document editor for creating and analyzing targeted proteomics experiments. Bioinformatics 26, 966-968 (2010).
15. Proc, J.L., et al. A quantitative study of the effects of chaotropic agents, surfactants, and solvents on the digestion efficiency of human plasma proteins by trypsin. J. Proteome Res. 9, 5422-5437 (2010).
16. Berna, M., et al. Online immunoaffinity liquid chromatography/tandem mass spectrometry determination of a type II collagen peptide biomarker in rat urine: Investigation of the impact of collision-induced dissociation fluctuation on peptide quantitation. Anal. Biochem. 356, 235-243 (2006).