RhoC GTPase activation assay

Journal of Visualized Experiments

Volumn , Issue 42, 2010, Pages

  Lucey, Michelle ^a   Unger, Heather ^a   van Golen, Kenneth L ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

Brain; Issue 42; Labeling; Mouse; Neuroscience; Transplantation

Indexed keywords

EID: 80355142033     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2083     Document Type: Article

References (10)

1. van Golen, K.L., Wu, Z.F., Qiao, X.T., Bao, L.W., & Merajver, S.D., RhoC GTPase, a novel transforming oncogene for human mammary epithelial cells that partially recapitulates the inflammatory breast cancer phenotype. Cancer Res 60 (20), 5832-5838 (2000).
2. van Golen, K.L., Wu, Z.F., Qiao, X.T., Bao, L., & Merajver, S.D., RhoC GTPase overexpression modulates induction of angiogenic factors in breast cells. Neoplasia. 2 (5), 418-425 (2000).
3. van Golen, K.L. et al., Reversion of RhoC GTPase-induced inflammatory breast cancer phenotype by treatment with a farnesyl transferase inhibitor. Mol Cancer Ther 1 (8), 575-583 (2002).
4. van Golen, K.L. et al., Mitogen activated protein kinase pathway is involved in RhoC GTPase induced motility, invasion and angiogenesis in inflammatory breast cancer. Clin Exp Metastasis 19 (4), 301-311 (2002).
5. Yao, H., Dashner, E.J., van Golen, C.M., & van Golen, K.L., RhoC GTPase is required for PC-3 prostate cancer cell invasion but not motility. Oncogene 25 (16), 2285-2296 (2006).
6. Hall, C.L. et al., Type I Collagen receptor (alpha2beta1) signaling promotes prostate cancer cell invasion through RhoC GTPase. Neoplasia. 10 (8), 797-803 (2008).
7. Sequeira, L., Dubyk, C.W., Riesenberger, T.A., Cooper, C.R., & van Golen, K.L., Rho GTPases in PC-3 prostate cancer cell morphology, invasion and tumor cell diapadesis. Clin Exp Metastasis 25 569-579 (2008).
8. van Golen, K.L. et al., CCL2 Induces Prostate Cancer Transendothelial Cell Migration via Activation of the Small GTPase Rac. J Cell Biochem 104, 1587-1597 (2008).
9. Chatterjee, M. & van Golen, K.L., Int J. Cancer, 2010 (submitted).
10. Sander, E.E., ten Klooster, J.P., van Delft, S., van der Kammen, R.A., & Collard, J.G., Rac downregulates Rho activity: reciprocal balance between both GTPases determines cellular morphology and migratory behavior. J Cell Biol 147 (5), 1009-1022 (1999).