Respirometric oxidative phosphorylation assessment in saponin-permeabilized cardiac fibers

Journal of Visualized Experiments

Volumn , Issue 48, 2010, Pages

  Hughey, Curtis C ^a   Hittel, Dustin S ^a   Johnsen, Virginia L ^a   Shearer, Jane ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

Cardiac fibers; Issue 48; Methodology; Mitochondria; Mouse; Oxygen consumption; Physiology

Indexed keywords

EID: 80355142021     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2431     Document Type: Article

References (20)

1. Saks, V.A., Veksler, V.I., Kuznetsov, A.V., et al. Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol Cell Biochem. 184(1-2):81-100 (1998).
2. Gnaiger, E., Kuznetsov, A.V., Schneeberger, S., et al. Mitochondria in the cold. In: Heldmaier G, Klingenspor M, eds. Life in the Cold. X ed: Springer, Berlin, Heidelberg, New York; 431-442 (2000).
3. Rasmussen, H.N., Rasmussen, U.F. Oxygen solubilities of media used in electrochemical respiration measurements. Anal Biochem. 319(1):105-113 (2003).
4. De Visscher, G., Rooker, S., Jorens, P., et al. Pentobarbital fails to reduce cerebral oxygen consumption early after non-hemorrhagic closed head injury in rats. J Neurotrauma. 22(7):793-806 (2005).
5. Kuznetsov, A.V., Veksler, V., Gellerich, F.N., et al. Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells. Nat Protoc. 3(6):965-976 (2008).
6. Gnaiger, E. Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology. Adv Exp Med Biol. 543:39-55 (2003).
7. Gnaiger, E. Capacity of oxidative phosphorylation in human skeletal muscle: new perspectives of mitochondrial physiology. Int J Biochem Cell Biol. 41(10):1837-1845(2009).
8. Sena, S., Hu, P., Zhang, D., et al. Impaired insulin signaling accelerates cardiac mitochondrial dysfunction after myocardial infarction. J Mol Cell Cardiol. 46(6):910-918 (2009).
9. Boudina, S., Sena, S., O'Neill, B.T., et al. Reduced mitochondrial oxidative capacity and increased mitochondrial uncoupling impair myocardial energetics in obesity. Circulation. 112(17):2686-2695 (2005).
10. Lenaz, G., Genova, M.L. Structure and organization of mitochondrial respiratory complexes: a new understanding of an old subject. Antioxid Redox Signal. 12(8):961-1008.
11. Lemieux, H., Hoppel, C.L. Mitochondria in the human heart. J Bioenerg Biomembr. 41(2):99-106 (2009).
12. Saks, V.A., Vasil'eva, E., Belikova Yu. O., et al. Retarded diffusion of ADP in cardiomyocytes: possible role of mitochondrial outer membrane and creatine kinase in cellular regulation of oxidative phosphorylation. Biochim Biophys Acta. 1144(2):134-148 (1993).
13. Endo, M., Kitazawa, T. E-C coupling studies in skinned cardiac fibers. In: Morad M, ed. Biophysical Aspects of Cardiac Muscle. New York: Academic; 307-327 (1978).
14. Veksler, V.I., Kuznetsov, A.V., Sharov, V.G., et al. Mitochondrial respiratory parameters in cardiac tissue: a novel method of assessment by using saponin-skinned fibers. Biochim Biophys Acta. 892(2):191-196 (1987).
15. Bangham, A.D., Horne, R.W., Glauert, A.M., et al. Action of saponin on biological cell membranes. Nature. 196:952-955 (1962).
16. Daum, G. Lipids of mitochondria. Biochim Biophys Acta. 822(1):1-42 (1985).
17. Milner, D.J., Mavroidis, M., Weisleder, N., et al. Desmin cytoskeleton linked to muscle mitochondrial distribution and respiratory function. J Cell Biol. 150(6):1283-1298 (2000).
18. Skladal, D., Sperl, W., Schranzhofer, R., et al. Preservation of mitochondrial functions in human skeletal muscle during storage in high energy preservation solution (HEPS). In: What is Controlling Life? In: Gnaiger E, Gellerich F, Wyss M, eds. Modern Trends in BioThermoKinetics. Vol 3: Innsbruck Univ. Press; 268-271 (1994).
19. Kuznetsov, A.V., Wiedemann, F.R., Winkler, K., et al. Use of saponin-permeabilized muscle fibers for the diagnosis of mitochondrial diseases. Biofactors. 7(3):221-223 (1998).
20. Gnaiger, E. Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Dykens J, Will Y, eds. Drug-Induced Mitochondrial Dysfunction: John Wiley & Sons, Inc. 327-352 (2008)